Chemiluminescent Measurement of Hydrogen Peroxide in the Exhaled Breath Condensate of Healthy and Asthmatic Adults

Abstract

Reactive oxygen species are centrally involved in the pathophysiology of airway diseases such as asthma and chronic obstructive pulmonary disease. This study reports the development of a chemiluminescence assay and a device for measuring hydrogen peroxide in the exhaled breath condensate of asthma patients and healthy participants. A stand-alone photon detection device was constructed for use with an optimized chemiluminescence assay. Calibrations using a catalase control to scavenge residual hydrogen peroxide in calibrant solutions provided analytically sensitive and specific measurements. We evaluated exhaled breath condensate hydrogen peroxide in 60 patients (ages 20-83; 30 healthy patients and 30 asthma patients) recruited from the John Peter Smith Hospital Network. The exhaled breath condensate hydrogen peroxide concentrations trended toward higher values in asthma patients compared to healthy participants (mean 142.5 vs 115.5 nM; p = 0.32). Asthma patients who had not used an albuterol rescue inhaler in the past week were compared to those who had and showed a trend toward higher hydrogen peroxide levels (mean 172.8 vs 115.9 nM; p = 0.25), and these patients also trended toward higher hydrogen peroxide than healthy participants (mean 172.8 vs 115.5 nM; p = 0.14). This pilot study demonstrates the ability of the newly developed assay and device to measure exhaled breath condensate hydrogen peroxide in asthma patients and healthy participants. The trends observed in this study are in agreement with previous literature and warrant further investigation of using this system to measure exhaled breath condensate hydrogen peroxide for monitoring oxidative stress in asthma.

Seeking Illumination: The Path to Chemiluminescent 1,2-Dioxetanes for Quantitative Measurements and In Vivo Imaging

“…H 2 O 2 levels in exhaled breath condensate (EBC) samples from human volunteers determined using this platform were found to be in close agreement with the commercial Amplex Red assay. In collaboration with the start-up company BioLum Sciences LLC, we recently evolved our chemiluminescent airway H 2 O 2 quantification platform into the BioSense 2.0 Laboratory module, a stand-alone photon detection device consisting of a photodiode, a 3D-printed adapter for disposable cuvettes, and a Bluetooth 4.0 data transmission module (Figure A, C) . Using this device, we studied the relationship between airway H 2 O 2 and asthma in EBC samples from 60 adults.…”

“…(C) The BioSense 2.0 Laboratory Module was used to measure H 2 O 2 concentrations in asthma patients and healthy volunteers in the John Peter Smith Hospital . Adapted with permission from ref . Copyright 2020 American Chemical Society.…”

“…In collaboration with the start-up company BioLum Sciences LLC, we recently evolved our chemiluminescent airway H 2 O 2 quantification platform into the BioSense 2.0 Laboratory module, a stand-alone photon detection device consisting of a photodiode, a 3D-printed adapter for disposable cuvettes, and a Bluetooth 4.0 data transmission module (Figure 13A, C). 83 Using this device, we studied the relationship between airway H 2 O 2 and asthma in EBC samples from 60 adults. Our results agreed with earlier literature showing trends of higher H 2 O 2 in asthma patients as compared to nonasthmatic patients.…”

Exhaled Breath Condensate—A Non-Invasive Approach for Diagnostic Methods in Asthma

“…H 2 O 2 levels were elevated in asthmatic patients, regardless of age [ 63 , 64 ], and even after pharmacological treatment patients with asthma presented higher levels of this compound in EBC [ 63 ]. H 2 O 2 concentrations in EBC increased in steroid-naive asthma and were influenced by the smoking habit [ 65 ] and disease treatment [ 66 , 67 ]. An in-house assessment of H 2 O 2 levels in EBC could be a promising evaluation method of asthma management and there was a successful attempt of using smartphones for analytic methods [ 68 ].…”

Co3O4 Nanoparticles Uniformly Dispersed in Rational Porous Carbon Nano-Boxes for Significantly Enhanced Electrocatalytic Detection of H2O2 Released from Living Cells

“…The excellent detection of H 2 O 2 mainly depends on the detection method and material two aspects. Among the technique for accurate and reliable detection of cellular H 2 O 2 , such as colorimetry [ 11 , 12 ], fluorescence [ 13 , 14 ], chromatography [ 15 ] and chemiluminescence [ 16 ], electrochemical techniques increasingly attracted attention due to their high sensitivity, good selectivity, low cost, as well as rapid response. For electrochemical detection, natural enzymes are usually the choice of sensing materials due to their remarkable specificity and high sensitivity in catalyzing the decomposition of H 2 O 2 .…”