Chemiluminescent simultaneous determination of phosphatidylcholine hydroperoxide and phosphatidylethanolamine hydroperoxide in the liver and brain of the rat.

Miyazawa, Teruo; Suzuki, Toshihide; Fujimoto, Kenshiro; Yasuda, K.

doi:10.1016/s0022-2275(20)41420-8

Cited by 128 publications

(12 citation statements)

References 43 publications

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“…The detection limit of the present HPLC-UV method was 4.5 ng (5 pmol) for Glc-PE, and 5.3 ng (5 pmol) for Lac-PE. Generally, the HPLC detection limit of phospholipid-derived chemical mediators, such as phospholipid hydroperoxides (21,22) and platelet-activating factor (PAF)-like phospholipids (23), is above picomole levels. Hence, the sensitivity of the present labeling method for Amadori-PE is relatively high.…”

Section: Discussionmentioning

confidence: 99%

UV analysis of Amadori-glycated phosphatidylethanolamine in foods and biological samples

Oak

Nakagawa

Miyazawa

2002

Journal of Lipid Research

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Maillard reactions are among the most important of the chemical and oxidative changes occurring in food and biological samples that contribute to food deterioration and to the pathophysiology of human disease. Although the association of lipid glycation with this process has recently been shown, the number of lipid glycation products in food and biological materials has not been clear. In this study, we synthesized the Amadori products derived from the glycation of phosphatidylethanolamine (PE), i.e., Amadori-PEs. Dioleoyl PE was incubated with glucose and lactose for 15 days, and the resultant Amadori-PEs were purified and isolated using solid phase extraction followed by HPLC. With this procedure, essentially pure ( Ͼ 98% purity) Amadori-PEs glycated with glucose (Glc-PE) and with lactose (Lac-PE) were obtained and used as standards in the subsequent studies.To determine the presence of Amadori-PEs in food and biological samples, the carbonyl group of Amadori-PEs was ultraviolet (UV)-labeled with 3-methyl-2-benzothiazolinone hydrazone, and the labeled Amadori-PEs were analyzed with normal phase HPLC-UV (318 nm). The detection limit was 4.5 ng (5 pmol) for Glc-PE and 5.3 ng (5 pmol) for Lac-PE. Among the several food samples examined, infant formula and chocolate contained a high amount of both Glc-PE and Lac-PE over wide concentration ranges, such as 1.5-112 g/g. Testing biological materials showed Amadori-PE (Glc-PE) was detectable in rat plasma. -Oak, J-H., K. Nakagawa, and T. Miyazawa. UV analysis of Amadoriglycated phosphatidylethanolamine in foods and biological samples.

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Section: Discussionmentioning

confidence: 99%

UV analysis of Amadori-glycated phosphatidylethanolamine in foods and biological samples

Oak

Nakagawa

Miyazawa

2002

Journal of Lipid Research

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“…A CL-HPLC method that incorporates a mixture of cytochrome c and luminol as a post-column hydroperoxidespecific luminescent reagent was used. 31) An npropylamine-bound silica column with a mixture of hexane-2-propanol-methanol-water 5 : 7 : 2 : 1 (by vol.) as an eluent was used to determine both PCOOH and PEOOH, which were separated from each other and from other lipids and lipid-soluble antioxidants.…”

Section: Development Of Cl-hplc For Measuring Lipid Hydroperoxides In Human Plasma and Tissue Organs Following Aging And Senescencementioning

confidence: 99%

Lipid hydroperoxides in nutrition, health, and diseases

Miyazawa

2021

Proc. Jpn. Acad., Ser. B

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Research on lipid peroxidation in food degradation, oil and fat nutrition, and age-related diseases has gained significant international attention for the view of improvement of societal health and longevity. In order to promote basic studies on these topics, a chemiluminescence detection-high performance liquid chromatography instrument using a high-sensitivity single photon counter as a detector was developed. This instrument enabled us to selectively detect and quantify lipid hydroperoxides, a primary product of lipid peroxidation reactions, as hydroperoxide groups at the lipid class level. Furthermore, an analytical method using liquid chromatography–tandem mass spectrometry has been established to discriminate the position and stereoisomerization of hydroperoxide groups in lipid hydroperoxides. Using these two methods, the reaction mechanisms of lipid peroxidation in food and in the body have been confirmed.

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“…The prooxidant activity of citrate-stabilised CeO 2 sol was analysed by a chemiluminescence method in a biochemical model containing oxidised phosphatidylcholine (PCOOH) and coumarin 334. Amounts of hydroperoxide groups in the phospholipid substrate were determined according to an established protocol 46 and were found to be 98 ± 10 μmol L −1 . An aqueous solution of deoxyhemoglobin (Fe( ii )) was used as a reference sample.…”

Section: Resultsmentioning

confidence: 99%

“… 45 The content of hydroperoxide in the samples of oxidised phosphatidylcholine was determined by HPLC with chemiluminescence detection. 46 …”

Section: Methodsmentioning

confidence: 99%

“…45 The content of hydroperoxide in the samples of oxidised phosphatidylcholine was determined by HPLC with chemiluminescence detection. 46 Analysis of prooxidant activity of CeO 2 nanoparticles by the chemiluminescent method Coumarin 334 (#393002, Sigma-Aldrich) was used as a chemiluminescent probe (CL probe) sensitive to lipid and phospholipid radicals. To obtain a working solution with a concentration of 5 mM, a weighed portion of coumarin 334 was dissolved in dimethyl sulfoxide ($99.95%, #472301, Sigma-Aldrich).…”

Section: Preparation Of Linoleic Acid and Phosphatidylcholine Hydroperoxidesmentioning

confidence: 99%

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New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles

et al. 2021

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Chemiluminescent simultaneous determination of phosphatidylcholine hydroperoxide and phosphatidylethanolamine hydroperoxide in the liver and brain of the rat.

Cited by 128 publications

References 43 publications

UV analysis of Amadori-glycated phosphatidylethanolamine in foods and biological samples

UV analysis of Amadori-glycated phosphatidylethanolamine in foods and biological samples

Lipid hydroperoxides in nutrition, health, and diseases

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles

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Chemiluminescent simultaneous determination of phosphatidylcholine hydroperoxide and phosphatidylethanolamine hydroperoxide in the liver and brain of the rat.

Cited by 128 publications

References 43 publications

UV analysis of Amadori-glycated phosphatidylethanolamine in foods and biological samples

UV analysis of Amadori-glycated phosphatidylethanolamine in foods and biological samples

Lipid hydroperoxides in nutrition, health, and diseases

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO2 nanoparticles

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Product

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New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles