Chemiluminometric evaluation of melatonin and selected melatonin precursors’ interaction with reactive oxygen and nitrogen species

Harasimowicz, Joanna; Marques, Karine L.; Silva, Ana Tavares da; Costa, Renata Cb; Prior, João A.V.; Rodrigues, S. Sofia M.; Santos, João L.M.

doi:10.1016/j.ab.2011.09.008

Cited by 16 publications

(8 citation statements)

References 18 publications

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“…The direct and indirect antioxidant properties of melatonin and its metabolites are well documented [18, 21, 23]. It was shown in numerous in vivo and in vitro studies that melatonin scavenges oxygen- and nitrogen-reactive species [21, 23, 68, 69], protects against lipid peroxidation [70, 71], and stimulates mRNAs and activities of antioxidant enzymes [72, 73]. …”

Section: Discussionmentioning

confidence: 99%

Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie‐Restricted Diet

Szewczyk-Golec

Rajewski²,

Gackowski

et al. 2017

Oxidative Medicine and Cellular Longevity

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Obesity is one of the major global health problems. Melatonin deficiency has been demonstrated to correlate with obesity. The aim of the study was to estimate the effect of melatonin on oxidative stress and adipokine levels in obese patients on a calorie-restricted diet. Thirty obese patients were supplemented with a daily dose of 10 mg of melatonin (n = 15) or placebo (n = 15) for 30 days with a calorie-restricted diet. Serum levels of melatonin, 4-hydroxynonenal (HNE), adiponectin, omentin-1, leptin, and resistin, as well as erythrocytic malondialdehyde (MDA) concentration and Zn/Cu-superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities, were measured at baseline and after supplementation. Significant body weight reduction was observed only in the melatonin group. After melatonin supplementation, the adiponectin and omentin-1 levels and GPx activities statistically increased, whereas the MDA concentrations were reduced. In the placebo group, a significant rise in the HNE and a drop in the melatonin concentrations were found. The results show evidence of increased oxidative stress accompanying calorie restriction. Melatonin supplementation facilitated body weight reduction, improved the antioxidant defense, and regulated adipokine secretion. The findings strongly suggest that melatonin should be considered in obesity management. This trial is registered with CTRI/2017/07/009093.

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Section: Discussionmentioning

confidence: 99%

Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie‐Restricted Diet

Szewczyk-Golec

Rajewski²,

Gackowski

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…Melatonin is an efficient direct antioxidant, scavenging effectively free radicals, peroxynitrite and hypochlorite (Reiter et al, ; Harasimowicz et al, ; Maitra et al, ). This effect does not seem to be too relevant physiologically, due to the low physiological concentrations of this hormone.…”

Section: Discussionmentioning

confidence: 99%

Role of melatonin receptor MT₂ and quinone reductase II in the regulation of the redox status of 3T3‐L1 preadipocytes in vitro

Adamczyk-Sowa

Sowa

Żwirska-Korczala

et al. 2013

Cell Biology International

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We have examined the role of melatonin receptor MT2 and quinone reductase II in the regulation of the redox status of preadipocytes (3T3-L1) in vitro. 3T3-L1 cells were treated with melatonin at a physiological concentration (10(-9) mol/L) and a supraphysiological (pharmacological) concentration (10(-3) mol/L) for 24 h. Luzindole (10(-4) mol/L), an antagonist of MT2 receptor, and prazosin (10(-5) mol/L), an inhibitor of quinone reductase II, were added 30 min before subsequent exposure of the cells to melatonin. The level of oxidative stress was determined by the analysis of activities of enzymes neutralising reactive oxygen species, and determination of the malondialdehyde (MDA) content. Melatonin increased activities of manganese and copper-zinc superoxide dismutase (MnSOD, Cu/ZnSOD) and catalase (CAT) at both a physiological concentration (10(-9) mol/L) and a pharmacological concentration (10(-3) mol/L). MDA content was unchanged, whereas activities of glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-Rd) were increased only by the physiological concentration. Both effects were partially inhibited by luzindole, but not prazosin. These observations suggest that melatonin, acting at least partially via MT2 receptors, can increase antioxidant enzymes activities in 3T3-L1 preadipocytes.

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“…80 This discovery was followed shortly by a series of studies, conducted both in vitro 81,82 and in vivo, [83][84][85][86][87] which documented the ability of melatonin to quell oxidative damage to molecules, cells and tissues, including human cells. 88,89 Since then, there have been numerous reports confirming the ability of melatonin to directly scavenge oxygen-centered radicals and toxic reactive oxygen species (ROS) [90][91][92][93][94][95] and to diminish oxidative mutilation to key cellular macromolecules. [96][97][98][99][100][101][102] These direct free radical scavenging actions of melatonin and its metabolites have been summarized in a number of reviews, 103-105 so they will not be discussed in detail here.…”

Section: Preparing For Battle: Melatonin's Physiological Weaponsmentioning

confidence: 99%

Melatonin as an antioxidant: under promises but over delivers

Reíter

Mayo

Tan

et al. 2016

Journal of Pineal Research

1,312

1,040

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Melatonin is uncommonly effective in reducing oxidative stress under a remarkably large number of circumstances. It achieves this action via a variety of means: direct detoxification of reactive oxygen and reactive nitrogen species and indirectly by stimulating antioxidant enzymes while suppressing the activity of pro-oxidant enzymes. In addition to these well-described actions, melatonin also reportedly chelates transition metals, which are involved in the Fenton/Haber-Weiss reactions; in doing so, melatonin reduces the formation of the devastatingly toxic hydroxyl radical resulting in the reduction of oxidative stress. Melatonin's ubiquitous but unequal intracellular distribution, including its high concentrations in mitochondria, likely aid in its capacity to resist oxidative stress and cellular apoptosis. There is credible evidence to suggest that melatonin should be classified as a mitochondria-targeted antioxidant. Melatonin's capacity to prevent oxidative damage and the associated physiological debilitation is well documented in numerous experimental ischemia/ reperfusion (hypoxia/reoxygenation) studies especially in the brain (stroke) and in the heart (heart attack). Melatonin, via its antiradical mechanisms, also reduces the toxicity of noxious prescription drugs and of methamphetamine, a drug of abuse.Experimental findings also indicate that melatonin renders treatment-resistant cancers sensitive to various therapeutic agents and may be useful, due to its multiple antioxidant actions, in especially delaying and perhaps treating a variety of agerelated diseases and dehumanizing conditions. Melatonin has been effectively used to combat oxidative stress, inflammation and cellular apoptosis and to restore tissue function in a number of human trials; its efficacy supports its more extensive use in a wider variety of human studies. The uncommonly high-safety profile of melatonin also bolsters this conclusion. It is the current feeling of the authors that, in view of the widely diverse beneficial functions that have been reported for melatonin, these may be merely epiphenomena of the more fundamental, yet-to-be identified basic action(s) of this ancient molecule. K E Y W O R D Sdiseases of aging, drug toxicity, free radicals, ischemia/reperfusion, mitochondria-targeted antioxidant, organ transplantation, statins

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Chemiluminometric evaluation of melatonin and selected melatonin precursors’ interaction with reactive oxygen and nitrogen species

Cited by 16 publications

References 18 publications

Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie‐Restricted Diet

Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie‐Restricted Diet

Role of melatonin receptor MT₂ and quinone reductase II in the regulation of the redox status of 3T3‐L1 preadipocytes in vitro

Melatonin as an antioxidant: under promises but over delivers

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Chemiluminometric evaluation of melatonin and selected melatonin precursors’ interaction with reactive oxygen and nitrogen species

Cited by 16 publications

References 18 publications

Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie‐Restricted Diet

Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie‐Restricted Diet

Role of melatonin receptor MT2 and quinone reductase II in the regulation of the redox status of 3T3‐L1 preadipocytes in vitro

Melatonin as an antioxidant: under promises but over delivers

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Role of melatonin receptor MT₂ and quinone reductase II in the regulation of the redox status of 3T3‐L1 preadipocytes in vitro