Chemiluminometric measurement of NO and 77Se-NMR studies about the redox-sensitive behaviour of sodium selenite in the presence of peroxynitrite and H2O2/O2

Albrecht, Steffen; Zimmermann, Thomas; Scheller, D.; Grützmann, Robert; Saeger, H. D.; Distler, W.

doi:10.1002/(sici)1099-0801(199904)13:2<131::aid-bmc886>3.0.co;2-2

Cited by 3 publications

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“…Therefore, it is necessary to find simple, rapid, and sensitive methods for the evaluation of . Various methods for the determination of have been proposed in the recent literature, such as electron spin resonance (ESR) [6], liquid chromatogram (LC) [7], electrochemistry [8,9], and chemiluminescence measurements [10,11,12], most of which require expensive instruments and complicated operations. In comparison with those methods, spectrophotometry is more simple, rapid, facile, and inexpensive.…”

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confidence: 99%

Simple and rapid catalytic spectrophotometric determination of superoxide anion radical and superoxide dismutase activity in natural medical vegetables using phenol as the substrate for horseradish peroxidase

Tang

Wang

2004

Analytical and Bioanalytical Chemistry

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The coupling reaction of 4-aminoantipyrine (4-AAP) with phenol using the superoxide anion radical (O2-*) as oxidizing agent under the catalysis of horseradish peroxidase (HRP) was studied. Based on the reaction, O2-* produced by irradiating vitamin B2 (VB2) was spectrophotometrically determined at 510 nm. Under the optimum experimental conditions, the relationship between A510 and O2-* concentration was linear in the range 9.14x10(-6)-1.2x10(-4) mol L(-1). The detection limit was determined to be 1.37x10(-6) mol L(-1). A possible reaction mechanism was discussed. The effect of interferences and surfactants on the determination of O2-* was also investigated. The proposed method was applied to determine superoxide dismutase activity in garlic, scallion, and onion with satisfactory results.

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Section: Introductionmentioning

confidence: 99%

Simple and rapid catalytic spectrophotometric determination of superoxide anion radical and superoxide dismutase activity in natural medical vegetables using phenol as the substrate for horseradish peroxidase

Tang

Wang

2004

Analytical and Bioanalytical Chemistry

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Measurement of free radicals and NO by chemiluminescence to identify the reperfusion injury in renal transplantation

et al. 2002

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Oxygen free radicals are generated during the reperfusion of ischaemic organs. Several experimental studies have demonstrated that the damage produced by reperfusion can be prevented by a scavenger of free radicals. Furthermore, a significantly improved 5 year graft survival rate after cadaveric renal transplantation has been reported in patients treated with scavengers of free radicals (Land et al., 1993). Therefore, a question remains to be answered: whether a routine monitoring of the radical-mediated reperfusion injury with renal transplantation is useful, and whether there is a necessity for a generalized protective treatment in transplant patients. In a prospective trial, we evaluated a group of eight patients during and after renal cadaveric transplantation (three men, five women), using the chemiluminometric measurement of serum free radicals and NO. The serum quantities of free radicals and NO were significantly increased after reperfusion of the transplant kidney (p < 0.02). The mean time of noticeably increased levels of serum free radicals was 4.8 +/- 1.2 h after reperfusion. The results thus showed an increased liberation of free radicals in the peripheral blood of transplant recipients as possible evidence of free radicals-mediated reperfusion injury in renal transplantation. The generation of free radicals measured by chemiluminescence allow a controlled therapy to decrease the generation of free radicals with antioxidants during the early transplantation period e.g. in older recipients.

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Zum redoxsensitiven Verhalten von Selenit in Gegenwart reaktiver Sauerstoffspezies

et al. 1999

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From extensive research over the last decade it has been known that selenium is essential as necessary component of selenoaminoacids and of specific enzymes. Among others, the redoxpair GSH/GSSG is closely connected with antioxidative processes. Moreover it inhibits and/or activates molecular key reactions with the involvement of various small reactive O- and N-species. We investigated the direct interaction of selenite with components of the respiratory burst of human blood cells, considering the redoxamphoterie of alkali-selenite. Selenite tend to redox-disproportation depending on the pH-value. Whether selenite leads to oxidation or reductation is dependent not only on the pH-value, but also on the redox-potential of the reaction partners. In in-vivo adapted in-vitro conditions (ph = 7.4; mumolar concentrations of reaction partners) we observed the following results: 1. SeO3(2-) is not oxidized by H2O2/NO or triplet-oxygen, when the oxidatives are applied alone; 2. SeO3(2-) is quantitatively oxidized from SeO4(2-) by the combination H2O2/NO2- or O2-/NO; 3. SeO3(2-) is semiquantitatively oxidized by singlett oxygen to SeO4(2-). The composition of reaction products was measured by 77Se-NMR-spectroscopy. The reactive intermediate product for the 2. reaction should be peroxynitrite (HOONO). One cannot rule out the possibility that HOONO reacts on a large scale with H2O2 to singlett oxygen. Subsequently singlett oxygen oxidizes selenite. The pathophysiological impact of singlett oxygen in processes like arteriosclerosis is now being investigated. It has been supposed, that singlett oxygen is participating in processes of lipidperoxidation invivo. Further investigations have to show, to what extent selenite is effective as direct 1O2-scavanger.

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Chemiluminometric measurement of NO and 77Se-NMR studies about the redox-sensitive behaviour of sodium selenite in the presence of peroxynitrite and H2O2/O2

Cited by 3 publications

References 4 publications

Simple and rapid catalytic spectrophotometric determination of superoxide anion radical and superoxide dismutase activity in natural medical vegetables using phenol as the substrate for horseradish peroxidase

Simple and rapid catalytic spectrophotometric determination of superoxide anion radical and superoxide dismutase activity in natural medical vegetables using phenol as the substrate for horseradish peroxidase

Measurement of free radicals and NO by chemiluminescence to identify the reperfusion injury in renal transplantation

Zum redoxsensitiven Verhalten von Selenit in Gegenwart reaktiver Sauerstoffspezies

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