ChemInform Abstract: Conversion of Imino‐1,2,3‐dithiazoles into 2‐Cyanobenzothiazoles, Cyanoimidoyl Chlorides and Diatomic Sulfur.

English, Russell F.; Ракитин, Олег А.; Rees, Charles W.; Vlasova, O. G.

doi:10.1002/chin.199724139

Cited by 1 publication

(2 citation statements)

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“…All reagents and solvents were purchased from commercial suppliers and used as received. Appel's salt, 4,5‐dichloro‐1,2,3‐dithiazolium chloride ( 6 ) was either purchased from LabNetwork (CAS #: 75318–43‐3) or prepared according to literature precedent 39–41 . Lugal, d ‐luciferin‐6‐O‐β‐ d ‐galactopyranoside, was purchased from Biosynth Carbosynth® (CAS #: 131474‐38‐9).…”

Section: Methodsmentioning

confidence: 99%

“…Appel's salt, 4,5-dichloro-1,2,3-dithiazolium chloride (6) was either purchased from LabNetwork (CAS #: 75318-43-3) or prepared according to literature precedent. [39][40][41] Lugal, d-luciferin-6-O-βd-galactopyranoside, was purchased from Biosynth Carbosynth® (CAS #: 131474-38-9). Other compounds were prepared according to literature precedent.…”

Section: General Synthetic Informationmentioning

confidence: 99%

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Caged luciferins enable rapid multicomponent bioluminescence imaging

Navarro

Brennan

Love

et al. 2023

Photochem & Photobiology

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Bioluminescence is a sensitive technique for imaging biological features over time. Historically, though, the modality has been challenging to employ for multiplexed tracking due to a lack of resolvable luciferase–luciferin pairs. Recent years have seen the development of numerous orthogonal probes for multi‐parameter imaging. While successful, generating such tools often requires complex syntheses and lengthy enzyme evolution campaigns. This work showcases an alternative strategy for multiplexed bioluminescence that takes advantage of already‐orthogonal caged luciferins and established uncaging enzymes. These probes generate unique bioluminescent signals that can be distinguished via a linear unmixing algorithm. Caged luciferins enabled two‐ and three‐component imaging on the minutes time scale. We further showed that the tools can be used in conjunction with endogenous enzymes for multiplexed studies. Collectively, this approach lowers the barrier to multicomponent bioluminescence imaging and can be readily adopted by the broader community.

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Section: Methodsmentioning

confidence: 99%

Section: General Synthetic Informationmentioning

confidence: 99%