ChemInform Abstract: Organophosphate Hydrolase — An Enzyme Catalyzing Degradation of Phosphorus‐Containing Toxins and Pesticides

Ефременко, Елена; Сергеева, В. С.

doi:10.1002/chin.200229286

Cited by 9 publications

(12 citation statements)

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“…В то же время не существует эф фективной и приемлемой для окружающей среды технологии утилизации этих веществ, в том числе в случае аварийных ситуаций (розливов, развалов и т.п.) [5,6].…”

Section: использование биокатализаторов на ос нове ферментов и микрооunclassified

“…В качестве биока тализаторов особый интерес представляют различные ферменты, гидролизующие ФОС. Было установлено, что на сегодняшний день наиболее высокоэффективно действующим ферментом для биодеструкции ФОС является органофосфатгидролаза (ОФГ, EC 3.1.8.1, арил диалкилфосфатаза), катализирующая гидролиз эфирной связи в триэфирах ортофосфорной и фосфоновой кислот [5,6].…”

Section: использование биокатализаторов на ос нове ферментов и микрооunclassified

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Research in the Sphere of Perspective Use of Biochemical and Medical Biocatalytic Technologies in the Interests of Armed Forces

2018

Journal of NBC Protection Corps

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The article is dedicated to the review of the theoretical and experimental research of the Russian and foreign scientists in the enzymes both in native state and in forms of chemically stabilized nano- sized particles, promising for the development of military products of different types. It summarises the results of use of biocatalysts on the basis of enzymes and microorganisms-destructors for the neutralization of eco-toxicants. The nature of enzymes is analyzed. The significant part of the article is dedicated to the data obtained regarding the sphere of biochemical and medical biocatalytic technologies for the development of enzymatic prophylactic and therapeutic means. A special attention is paid to the enzymes, used in protective equipment, to environmental biocatalysts, to biopharmaceuticals on the basis of enzymes and microorganisms-destructors for the utilization of reaction masses, to chemical detoxification of poisonous substances. The authors point out the main trends in the further research in the sphere of biotechnologies: enzymatic pharmaceuticals for prophylaxis and treatment of OP poisoning; enzymes in self-degassing materials and in protective equipment; biocatalysts for soil, water and surface purification; biopharmaceuticals on the basis of enzymes and microorganisms-destructors for the degradation of reaction masses of toxic chemicals.

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Section: использование биокатализаторов на ос нове ферментов и микрооunclassified

Research in the Sphere of Perspective Use of Biochemical and Medical Biocatalytic Technologies in the Interests of Armed Forces

2018

Journal of NBC Protection Corps

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“…), and some chemical warfare agents (sarin, soman, tabun, VX, etc.) act as substrates for the enzyme organophosphorus hydrolase (OPH) [42][43][44]. The enzyme catalyzed hydrolysis of these substances yields p-nitrophenol (PNP).…”

Section: Substrate Based Electrochemical Biosensors For Ops Quantificmentioning

confidence: 99%

Recent Trends in the Development of Electrochemical Biosensors for Organophosphorus Pesticides Determination

Stoytcheva¹,

Zlatev²

2014

Modern Electrochemical Methods in Nano, Surface and Corrosion Science

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“…Non-chromatographic methods have been described in the literature for the determination of several organophosphorus pesticides found in formulations, soils, crops or water. These are based on UV-visible [7] or infrared spectroscopy [8], solid phase extraction [9] or enzymatic techniques [10], sometimes in combination with mass spectrometry (MS) [11] or the flow injection technique [12].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence and Electrochemical Sensing of Pesticides Methomyl, Aldicarb and Prometryne by the Luminescent Europium-3-Carboxycoumarin Probe

et al. 2011

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This work describes the application of time resolved fluorescence in microtiterplates and electrochemical methods on glassy carbon electrode for investigating the interactions of europium-3-carboxycoumarin with pesticides aldicarb, methomyl and prometryne. Stern-volmer studies at different temperatures indicate that static quenching dominates for methomyl, aldicarb and prometryne. By using Lineweaver-Burk equation binding constants were determined at 303 K, 308 K and 313 K. A thermodynamic analysis showed that the reaction is spontaneous with ΔG being negative. The enthalpy ΔH and the entropy ΔS of reactions were all determined. A time-resolved (gated) luminescence-based method for determination of pesticides in microtiterplate format using the long-lived europium-3-carboxycoumarin has been developed. The limit of detection is 4.80, 5.06 and 8.01 μmol L(-1) for methomyl, prometryne and aldicarb, respectively. This is the lowest limit of detection achieved so far for luminescent lanthanide-based probes for pesticides. The interaction of the probe with the pesticides has been investigated using cyclic voltammetry (CV), differential pulse polarography (DPP), square wave voltammetry (SWV) and linear sweep voltammetry (LSV) on a glassy carbon electrode in I = 0.1 mol L(-1) p-toluenesulfonate at 25 °C. The diffusion coefficients of the reduced species are calculated. The main properties of the electrode reaction occurring in a finite diffusion space are the quasireversible maximum and the splitting of the net SWV peak for Eu(III) ions in the ternary complex formed . It was observed that the increase of the cathodic peak currents using LSV is linear with the increase of pesticides concentration in the range 5 × 10(-7) to 1 × 10(-5) mol L(-1). The detection limit (DL) were about 1.01, 2.23 and 1.89 μmol L(-1) for aldicarb, methomyl and prometryne, respectively. In order to assess the analytical applicability of the method, the influence of various potentially interfering species was examined. Influence of interfering species on the recovery of 10 μmol L(-1) pesticides has been investigated.

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ChemInform Abstract: Organophosphate Hydrolase — An Enzyme Catalyzing Degradation of Phosphorus‐Containing Toxins and Pesticides

Cited by 9 publications

References 1 publication

Research in the Sphere of Perspective Use of Biochemical and Medical Biocatalytic Technologies in the Interests of Armed Forces

Research in the Sphere of Perspective Use of Biochemical and Medical Biocatalytic Technologies in the Interests of Armed Forces

Recent Trends in the Development of Electrochemical Biosensors for Organophosphorus Pesticides Determination

Fluorescence and Electrochemical Sensing of Pesticides Methomyl, Aldicarb and Prometryne by the Luminescent Europium-3-Carboxycoumarin Probe

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