Abstract. Organic waste, fruit waste and vegetable waste are the best substrate to produce biogas. Waste management system for producing biogas can be used as a solution with the waste problem by converting the wastes into biogas. This study is expected to review of the effect of substrate type and substrate composition for the volume of biogas produced. In this study, materials consist of fruit wastes (oranges, apples, papayas, and tomatoes), cow ruminant, urea, cow dung, Na 2 CO 3 buffer, NH 4 HCO 3 buffer, and distilled water with variations of the substrate materials, F/ W, and the buffer types. The addition of cow manure and Na 2 CO 3 buffer with 1:2 of F/W, production of biogas is greater than variable which is used NH 4 HCO 3 buffer and without the addition of cow dung. Variables with addition of cow dung with 1:1 of F/W and using Na 2 CO 3 buffer, the result is greater than the variable using the same buffer but without the addition of cow dung and variables with 1:1 of F/W with the addition of cow dung and Na 2 CO 3 buffer and variables with the same feed and without the addition of cow dung produce more biogases than variable which is the using NH 4 HCO 3 buffer, 1:1 of F/W and without the addition of cow dung.