ChemInform Abstract: SYNTHESIS AND PROPERTIES OF THE α‐KETO ACIDS

Cooper, Arthur J. L.; Ginos, James Z.; Meister, Anne C.

doi:10.1002/chin.198412366

Cited by 15 publications

(27 citation statements)

References 1 publication

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“…Reference to my work given by Wu & Thompson in this connection did not involve a measurement of the transamination between methionine and a 2-oxo acid [3]. In fact, I measured transamination between Lphenylalanine and 2-oxo-4-methylthiobutyrate (ameasure of glutamine transaminase K activity) and between Lalbizziin and 2-oxo-4-methylthiobutyrate (a measure of glutamine transaminase L activity).…”

Section: Methionine Transamination In Vivomentioning

confidence: 99%

“…However, we have pointed out on several occasions (e.g. [3][4][5][6]) that glutamine is likely to be the amino acid substrate in vivo. Glutamine is generally present in tissues at a much higher concentration than methionine.…”

Section: Methionine Transamination In Vivomentioning

confidence: 99%

“…In a more recent study, gas-liquid chromatography was used to measure serum and urinary products of the methionine transamination pathway [13]. It was concluded that, in normal individuals, the transamination pathway (although it exists) is not a quantitatively important route for methionine breakdown, even after methionine loading [3].…”

Section: Methionine Transamination In Vivomentioning

confidence: 99%

“…Mitochondrial and cytosolic fractions of skeletal muscles from 60-80 g fed Sprague-Dawley female rats and 115-130 g fed male broiler chicks (Gallus domesticus) were prepared as previously described [8]. Glutamine transaminase K was assayed in the presence of 10 mM-glutamine, 50,aM-phenylpyruvate and 50 mM-sodium borate (pH 8.5) as described by Cooper & Meister [3][4][5], except that phenylalanine was measured by h.p.l.c. [2].…”

Section: Methionine Transamination In Vivomentioning

confidence: 99%

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Methionine transamination in vivo

Cooper¹

1989

Biochemical Journal

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Section: Methionine Transamination In Vivomentioning

confidence: 99%

Section: Methionine Transamination In Vivomentioning

confidence: 99%

Section: Methionine Transamination In Vivomentioning

confidence: 99%

Section: Methionine Transamination In Vivomentioning

confidence: 99%

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Methionine transamination in vivo

Cooper¹

1989

Biochemical Journal

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“…α-Keto acids are used as model substrates of enzymes and in the development of enzyme inhibitors. 3 They have been used to treat some pathological conditions, 4, 5, 6 and are of interest as biosynthetic precursors 7 and intermediates in the syntheses. 8 …”

Section: Introductionmentioning

confidence: 99%

Determination of pKa and Hydration Constants for a Series of α-Keto-Carboxylic Acids Using Nuclear Magnetic Resonance Spectrometry

Lopalco

Douglas

Denora

et al. 2016

Journal of Pharmaceutical Sciences

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The determination of the acid-base dissociation constants, and thus the pKa values, of α-keto acids such as pyruvic acid is complex due to the existence of these acids in their hydrated and non-hydrated or oxo-state. Equilibria involved in the hydration and dehydration of the α-keto group of pyruvic acid and three other α-keto acids, 3-methyl-2-oxobutanoic acid, 4-methyl-2-oxopentanoic acid, and 2-oxo-2-phenylacetic acid, were investigated by proton and carbon nuclear magnetic resonance spectrometry, NMR, at constant ionic strength, 0.15, and 25°C. Dissociation constants for the oxo (pKaoxo) and hydrated (pKahyd) acids of each compound were estimated from the change in the degree of hydration with changes in pH and directly from the changes in chemical shifts of various hydrogen and carbons nuclei with pH. α-Keto acids showed greater hydration in their acidic forms than their carboxylate forms. The degree of hydration was sensitive to steric and electronic/resonance factors. As expected, the oxo forms of the acids were stronger acids compared with their hydrated analogs, and their dissociation constants were also sensitive to steric and electronic factors.

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Biomarkers of Mycoplasma pneumoniae pneumonia in children by urine metabolomics based on Q Exactive liquid chromatography/tandem mass spectrometry

Wei

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale: Mycoplasma pneumoniae has become one of the common pathogens causing pediatric respiratory infections. In clinical diagnosis, throat swabs are very difficult to obtain from children, and there is a possibility of false positive results; hence, there are few clinically available diagnostic methods. Methods: In this study, Q Exactive liquid chromatography/tandem mass spectrometry was used to analyze the metabolites in the urine of healthy children (HC) and M. pneumoniae pneumonia in children (MPPC) patients. A multivariate statistical analysis was performed to screen the differential metabolites. Based on the HMDB and KEGG, the possible metabolic pathways subject to biological alteration were identified. Results: Compared with HC, 73 different metabolites in MPPC patients disrupted nine metabolic pathways through different change trends; after integrating various parameters, 20 significantly different metabolites were identified as MPPC potential biomarkers. Through the above two analysis modes, acetylphosphate and 2,5-dioxopentanoate were both screened out and used as potential biomarkers for the early diagnosis of MPPC for the first time. Conclusions: The characterization of 20 potential biomarkers provides a scientific basis for predicting and diagnosing MPPC. This article further indicates that urine metabolic profiling has great potential in diagnosing MPPC and can effectively prevent the disease from causing further deterioration.

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ChemInform Abstract: SYNTHESIS AND PROPERTIES OF THE α‐KETO ACIDS

Cited by 15 publications

References 1 publication

Methionine transamination in vivo

Methionine transamination in vivo

Determination of pKa and Hydration Constants for a Series of α-Keto-Carboxylic Acids Using Nuclear Magnetic Resonance Spectrometry

Biomarkers of Mycoplasma pneumoniae pneumonia in children by urine metabolomics based on Q Exactive liquid chromatography/tandem mass spectrometry

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