Chemistry and biocompatibility of alginate‐PLL capsules for immunoprotection of mammalian cells

Vos, Paul de; Hoogmoed, Chris G. van; Busscher, Henk J.

doi:10.1002/jbm.10060

Cited by 104 publications

(115 citation statements)

References 35 publications

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“…In previous studies we showed that especially variations in poly-L-lysine content, i.e. Ncontent, can be hold responsible for inflammatory responses [34,42]. In the present study we therefore engineered capsules with a similar N-content (Table 1).…”

Section: Surface Properties Before Implantationmentioning

confidence: 95%

“…This decrease in N signal reflects a loss of PLL at the surface of high G capsules and exposure of positive charges. Free positive charges on high-G capsules are considered to be responsible for inflammatory responses and are considered to be causative for inflammatory responses to alginate-PLL capsules [34,47]. To measure possible positive charges on capsules we quantified the zeta-potential of implanted capsules at day 1 after implantation.…”

Section: Surface Properties After Implantationmentioning

confidence: 99%

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The association between in vivo physicochemical changes and inflammatory responses against alginate based microcapsules

et al. 2012

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Citation for published version (APA): de Vos, P., Spasojevic, M., de Haan, B., & Faas, M. M. (2012). The association between in vivo physicochemical changes and inflammatory responses against alginate based microcapsules. Biomaterials, 33(22), 5552-5559. a b s t r a c t Application of alginate-polylysine (PLL) capsules for immunoisolation of living cells are suffering from a varying degree of success and large lab-to-lab variations. In this study we show that these differences in success rates can be attributed to alginate dependent essential physicochemical changes of the properties of capsules in vivo that will render the capsules more susceptible to inflammatory responses. Capsule properties were studied before and after implantation by XPS, by immunocytochemistry, and by measuring zeta potentials. We studied a capsule type which provokes for unknown reasons a strong inflammatory response, i.e. high-guluronic (G) alginate capsules and a capsule type with near identical physicochemical properties but which evokes a minimal inflammatory response, i.e. intermediate-G alginate capsules. The cause of the difference in response was a decrease in nitrogen content on high-G capsules due to detachment of PLL in vivo and an increase of the zeta-potential. Our data illustrate an important overlooked phenomena; the physicochemical properties are not necessarily the properties after exposure to the in vivo microenvironment and might induce undesired inflammatory responses and failure of encapsulated cellular grafts.

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Section: Surface Properties Before Implantationmentioning

confidence: 95%

Section: Surface Properties After Implantationmentioning

confidence: 99%

The association between in vivo physicochemical changes and inflammatory responses against alginate based microcapsules

et al. 2012

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“…Specifically, the challenges used to overcome the functional loss of glucose sensors, restenosis after stent implantation, and calcification induced by implantable devices are discussed. chitosan, [20][21][22] alginate, 23 hyaluronan, 24 and dextran. 25,26 Commonly used synthetic polymers include poly(lactic acid)(PLA) and poly(lactic-co-glycolic acid)(PLGA), [27][28][29][30][31][32][33] poly(ethylene glycol)(PEG), 34-36 2-hydroxy ethyl methacrylate, 33 and poly(vinyl alcohol)(PVA).…”

Section: Symposium Abstractmentioning

confidence: 99%

A Review of the Biocompatibility of Implantable Devices: Current Challenges to Overcome Foreign Body Response

Onuki

Bhardwaj

Papadimitrakopoulos

et al. 2008

J Diabetes Sci Technol

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In recent years, a variety of devices (drug-eluting stents, artificial organs, biosensors, catheters, scaffolds for tissue engineering, heart valves, etc.) have been developed for implantation into patients. However, when such devices are implanted into the body, the body can react to these in a number of different ways. These reactions can result in an unexpected risk for patients. Therefore, it is important to assess and optimize the biocompatibility of implantable devices. To date, numerous strategies have been investigated to overcome body reactions induced by the implantation of devices. This review focuses on the foreign body response and the approaches that have been taken to overcome this. The biological response following device implantation and the methods for biocompatibility evaluation are summarized. Then the risks of implantable devices and the challenges to overcome these problems are introduced. Specifically, the challenges used to overcome the functional loss of glucose sensors, restenosis after stent implantation, and calcification induced by implantable devices are discussed.

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“…However, less cytotoxicity or inflammatory reactions (21). Alginate is the most commonly employed polymer the intraparenchymal administration is characterized by early death of grafted cells (23,29,38,44,48) and in the for cell encapsulation because of its excellent biocompatibility and an in vivo stability (6,40,53). Alginate is case of their survival they potentially differentiate in unsuitable phenotypes.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Alginate Microspheres for Mesenchymal Stem Cell Engraftment on Solid Organ

et al. 2010

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Mesenchymal stem cells (MSCs) may be used as a cell source for cell therapy of solid organs due to their differentiation potential and paracrine effect. Nevertheless, optimization of MSC-based therapy needs to develop alternative strategies to improve cell administration and efficiency. One option is the use of alginate microencapsulation, which presents an excellent biocompatibility and an in vivo stability. As MSCs are hypoimmunogenic, it was conceivable to produce microparticles with [alginate-poly-L-lysine-alginate (APA) microcapsules] or without (alginate microspheres) a surrounding protective membrane. Therefore, the aim of this study was to determine the most suitable microparticles to encapsulate MSCs for engraftment on solid organ. First, we compared the two types of microparticles with 4 × 10 6 MSCs/ml of alginate. Results showed that each microparticle has distinct morphology and mechanical resistance but both remained stable over time. However, as MSCs exhibited a better viability in microspheres than in microcapsules, the study was pursued with microspheres. We demonstrated that viable MSCs were still able to produce the paracrine factor bFGF and did not present any chondrogenic or osteogenic differentiation, processes sometimes reported with the use of polymers. We then proved that microspheres could be implanted under the renal capsule without degradation with time or inducing impairment of renal function. In conclusion, these microspheres behave as an implantable scaffold whose biological and functional properties could be adapted to fit with clinical applications.

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Chemistry and biocompatibility of alginate‐PLL capsules for immunoprotection of mammalian cells

Cited by 104 publications

References 35 publications

The association between in vivo physicochemical changes and inflammatory responses against alginate based microcapsules

The association between in vivo physicochemical changes and inflammatory responses against alginate based microcapsules

A Review of the Biocompatibility of Implantable Devices: Current Challenges to Overcome Foreign Body Response

Evaluation of Alginate Microspheres for Mesenchymal Stem Cell Engraftment on Solid Organ

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