“…Screening of potential antimicrobial compounds from plants by clinical microbiologists is commonly performed with pure substances or crude extracts using broth dilution assay and the disc or agar well diffusion assay. Thus, these plant secondary metabolites have been demonstrated to possess a large spectrum of activity against pathogenic and non pathogenic bacteria (Shigella flexneri, diverse Staphylococcus, Streptococcus and Enterococcus species, Pseudomonas aeruginosa, Salmonella typhimurium, Mycobacterium tuberculosis, Klebsiella pneumonia, Escherichia coli etc) and fungal species as well as viruses like HIV (Saravanakumar et al, 2009) for review; (Liu, 2007;Mahady, 2005) and parasites like Trypanosoma and Plasmodium organisms (for reviews (Athanasiadou and Kyriazakis, 2004;Kokoska and Janovska, 2009). …”