“…Driven by these reasons, more in-depth anticancer activity studies of 2 and 9 were not carried out. Because some anticancer nucleosides may show their activity after a longer treatment time, 73 the MTT experiment was also carried out after 72 h of exposure; however, no changes in the IC 50 values were noticed (data not shown). In summary, the MTT assay showed that nucleosides 4–6 , 11 and 10 are appropriate for imaging in live HeLa cells with luminescence confocal microscopy due to their low cytotoxic activity.…”