“…Si gel column chromatography of the crude extract, followed by rechromatography of selected fractions exhibiting antimicrobial activity and/or containing compounds that could be interesting from a pharmacological as well as a phytochemical point of view (e.g., sesquiterpene lactones), afforded the four flavones, santin ( 1 ), centaureidin ( 2 ), apigenin ( 3 ), and its 7- O -β-glucoside ( 4 ) . In addition, nine sesquiterpene lactones, all belonging to a 12,6α-olide group (with 11αH configuration), have been isolated: 8α-hydroxy-1α,4α,5α,11βH-guaia-10(14)-en-12,6α-olide ( 5 , a new guaianolide), the elemanolide temisin ( 6 ), three germacranolides [balchanolide ( 7 ), 1β-hydroperoxy-8α-hydroxygermacra-4,10(14)-dien-6β,7α,11βH-12,6α-olide ( 8 ), shonachalin A ( 9 ) 13 ], and four eudesmanolides [11β,13-dihydroreynosin ( 10 ), 1β-hydroxy-6β,7α,11βH-selin-3-en-6,12-olide ( 11 ), 8α-hydroxy-11β,13-dihydrobalchanin ( 12 ), and artapshin ( 13 ) 15 ]. The lactones and flavones isolated previously from other plants were identified by comparison of their spectral data with the published ones.…”