Chemo-profiling of Pandanus odoratissimus L. fragrant inflorescence used in ayurveda, through HPTLC and GC

Abstract

Herbal material used in therapeutics contains a lot of chemical constituents which can be classified primarily as primary and secondary metabolites. Chemical evaluation of herbal material involves different chemical tests, assays, isolation, purification and there by identification of active constituents. Pandanus odoratissimus L. commonly known as Ketaki, the essential oil extracted out of the flowers beneficial in arthritis, antispasmodic and nootropic effect. Materials and Methods: Inflorescences of test drug separated from their colourful bracts shade dried for a day and cut in to small piece. For HPTLC n-hexane extract of this sample was applied on a precoated aluminium plates using Benzene as mobile phase. Developed plates were visualized in UV 254, 366, under white light and then derivatised with vanillin sulphuric acid and scanned under UV 254 and 366 nm. Methylated volatile oil of test sample was introduced into a stream of helium, for Gas Chromatography study. The results were tabulated as per the retention time. Results: HPTLC photo documentation of n-hexane extract of drug has shown 5 peaks at 254nm, whereas 6 peaks at 366nm. After post derivatisation at 620nm drug has shown 9 peaks. GC-MS analysis of volatile oil of test drug showed presence of totally 39 compounds among which 32 were identified by their Mass spectra with MS-Library.