Chemoenzymatic Synthesis of <i>Campylobacter jejuni</i> Lipo-oligosaccharide Core Domains to Examine Guillain–Barré Syndrome Serum Antibody Specificities

Li, Tiehai; Wolfert, Margreet A.; Wei, Na; Huizinga, Ruth; Jacobs, Bart C.; Boons, Geert‐Jan

doi:10.1021/jacs.0c08583

Cited by 37 publications

(27 citation statements)

References 55 publications

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“…Glycan array binding analysis of the rHAs was carried out as described here. 50,51 Briefly, recombinant hexahistidine-tagged HA was precomplexed with a mouse anti-his Alexa 647 antibody (Abcam) and goat - anti-mouse Alexa 647 antibodies. This was done in 50 μL PBS-T (phosphate-buffered saline with 0.1% Tween-20) in a 4:2:1 molar ratio, incubated for 15 minutes on ice, and the applied on the array for 90 minutes in a humidified chamber.…”

Section: Methodsmentioning

confidence: 99%

Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus

Arunkumar

Strohmeier

et al. 2021

Preprint

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A panel of novel influenza-like virus sequences were recently documented in jawless fish, ray-finned fish, and amphibians. Of these, the Wuhan spiny eel influenza virus (WSEIV) was found to phylogenetically cluster with influenza B viruses as a sister clade. Influenza B viruses have been historically documented to circulate only in humans, with certain virus isolates found in harbor seals. It is therefore interesting that a similar virus was potentially found in fish. Here we characterized the functionality and antigenicity of the putative hemagglutinin (HA) and neuraminidase (NA) surface glycoproteins of the WSEIV to better understand this virus and its pandemic potential. Upon functional characterization of NA, we identified that the WSEIV NA-like protein has sialidase activity comparable to B/Malaysia/2506/2004 influenza B virus NA, making it a bona fide neuraminidase that could be inhibited by NA inhibitors. Testing of the functionality of HA was carried out including receptor specificity, stability, and preferential airway protease cleavage and showed very specific binding to monosialic ganglioside 2 (GM2). To probe the degree of conservation of target epitopes, binding of known broadly cross-reactive monoclonal antibodies targeting the influenza B HA and NA, respectively, were assessed through enzyme linked immunosorbent assays against recombinant WSEIV glycoproteins. Human serum samples of patients with antibodies to influenza B viruses were used to determine the cross-reactivity against these novel glycoproteins. Very few monoclonal antibodies - notably including pan NA antibody 1G01 - showed cross-reactivity and reactivity from human sera was basically absent. In summary, we have conducted a functional and antigenic characterization of the glycoproteins of the novel WSEIV to assess if it is indeed a bona fide influenza virus potentially circulating in ray-finned fish.

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Section: Methodsmentioning

confidence: 99%

Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus

Arunkumar

Strohmeier

et al. 2021

Preprint

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“…Some examples that are of special interest for glycan mimicry are gangliosides and poly-LacNAc repeats that were synthesized through automated synthesis in combination with enzymes ( Li et al. 2019 ) or the LOS of C. jejuni that was synthesized through chemoenzymatic synthesis ( Li, Wolfert, et al. 2020 ).…”

Section: Chemical Glycobiology Techniques To Study Glycan Mimicrymentioning

confidence: 99%

Sweet impersonators: Molecular mimicry of host glycans by bacteria

2021

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All bacteria display surface-exposed glycans that can play an important role in their interaction with the host and in select cases mimic the glycans found on host cells, an event called molecular or glycan mimicry. In this review, we highlight the key bacteria that display human glycan mimicry and provide an overview of the involved glycan structures. We also discuss the general trends and outstanding questions associated with human glycan mimicry by bacteria. Finally, we provide an overview of several techniques that have emerged from the discipline of chemical glycobiology, which can aid in the study of the composition, variability, interaction and functional role of these mimicking glycans.

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“…Campylobacter jejuni is a gram-negative organism that is a common cause of bacterial diarrhea in developed and developing countries and is known as an infectious agent before the onset of neurological symptoms of Guillain-Barre syndrome. It is thought that due to the molecular similarity of the membrane antigens of this bacterium with peripheral nerve gangliosides, antibodies made against the bacterial membrane antigen cause damage to the peripheral nerves [12,13]. In addition to causing clinical symptoms, Guillain-Barre syndrome may be mistaken with poliomyelitis and since polio is close to eradication, all cases of acute flaccid paralysis, including Guillain-Barre syndrome, are being carefully examined [14].…”

Section: Introductionmentioning

confidence: 99%

Clinical Features of Guillain-Barre Syndrome in COVID-19 Patients: Aria and Naft Private Hospitals in Ahvaz, Iran

Bahramy¹,

Roozdar-Chaleshtary²,

Abbasi³

et al. 2021

Entomol. Appl. Sci. Lett.

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Given the importance of rapid diagnosis and rejection of multiple possible diagnoses and selection of appropriate treatment for Guillain-Barre syndrome in COVID-19 patients, the present descriptive crosssectional study investigated the clinical features of Guillain-Barre syndrome in COVID-19 patients. The statistical population of the present study was all Guillain-Barre patients with COVID-19 referred to Aria and Naft private hospitals in Ahvaz in 2020 and 2021. The data collection tool was a researcher-made checklist. Paired t-test and Chi-square test were used to determine the relationship between variables. Data was analyzed using SPSS24 software. The mean age of patients was 47 years. A high percentage of patients had paresthesia (87.5%) lower limb weakness and upper limb weakness was 75%. After hospitalization, 62.5% (5 patients) received IVIg, 12.5% (one patient) received plasmapheresis, and 25% (2 patients) received physiotherapy. Spinal fluid protein was in the range of 45-110 and cell count was in the normal range. IVIg treatment was 0.4 gr / kg / d for 5 days. Based on the results, it is recommended that IVIg treatment is considered along with other COVID-19 specific therapies. In addition, during the COVID-19 pandemic, an increase in GBS was observed in Khuzestan province, indicating the role of COVID-19 in GBS. It is necessary to hold educational courses to enhance information about this disease and its relevant symptoms and conduct studies with a larger sample of patients with COVID-19 at the national and provincial level.

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Chemoenzymatic Synthesis of Campylobacter jejuni Lipo-oligosaccharide Core Domains to Examine Guillain–Barré Syndrome Serum Antibody Specificities

Cited by 37 publications

References 55 publications

Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus

Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus

Sweet impersonators: Molecular mimicry of host glycans by bacteria

Clinical Features of Guillain-Barre Syndrome in COVID-19 Patients: Aria and Naft Private Hospitals in Ahvaz, Iran

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