Chemoenzymatic Synthesis of
 N
 -Acetyl-
 <scp>d</scp>
 -Neuraminic Acid from
 N
 -Acetyl-
 <scp>d</scp>
 -Glucosamine by Using the Spore Surface-Displayed
 N
 -Acetyl-
 <scp>d</scp>
 -Neuraminic Acid Aldolase

Gao, Chao; Xu, Xiaoman; Zhang, Xifeng; Che, Bin; Ma, Cuiqing; Qiu, Jianhua; Tao, Fei; Xu, Ping

doi:10.1128/aem.05601-11

Cited by 19 publications

(7 citation statements)

References 25 publications

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“…NeuAc production by the GRAS B. subtilis strain developed in this study should be more favorable for the production of food‐grade NeuAc. Furthermore, the production method developed in this study was more economically competitive compared to the production of NeuAc by B. subtilis spores with NanA displayed on the spore surface requiring GlcNAc or ManNAc as substrates as well as complex cell disruption and spore isolation methods . Our study demonstrated that the whole‐cell biocatalysis by B. subtilis is a promising method for the production of food‐grade NeuAc.…”

Section: Discussionmentioning

confidence: 84%

“…However, the NeuAc produced by engineered E. coli can be potentially contaminated by endotoxins, which may limit its application as a nutraceutical and a diet supplement . Therefore, NeuAc aldolase was displayed on the surface of Bacillus subtilis ( B. subtilis ) spores via a spore surface display system for the bioconversion of GlcNAc or ManNAc in combination with pyruvate to NeuAc . However, using ManNAc as a substrate and spore purification significantly increased the cost and complexity of NeuAc production, which reduced the economic feasibility for large‐scale NeuAc production.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pathway Engineering of Bacillus subtilis for Enhanced N‐Acetylneuraminic Acid Production via Whole‐Cell Biocatalysis

Zhao

Tian

Shen

et al. 2019

Biotechnology Journal

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N‐acetylneuraminic acid (NeuAc) is a common sialic acid that has a wide range of applications in nutraceuticals and pharmaceuticals. However, low production efficiency and high environmental pollution associated with traditional extraction and chemical synthesis methods constrain the supply of NeuAc. Here, a biological approach is developed for food‐grade NeuAc production via whole‐cell biocatalysis by the generally regarded as safe (GRAS) bacterium Bacillus subtilis (B. subtilis). Promoters for controlling N‐acetylglucosamine 2‐epimerase (AGE) and NeuAc adolase (NanA) are optimized, yielding 32.84 g L−1 NeuAc production with a molar conversion rate of 26.55% from N‐acetylglucosamine (GlcNAc). Next, NeuAc production is further enhanced to 46.04 g L−1, which is 40.2% higher than that of the strain with promoter optimization, by expressing NanA from Staphylococcus hominis instead of NanA from Escherichia coli. To enhance the expression level of ShNanA, the N‐terminal coding sequences of genes with high expression levels are fused to the 5′‐end of the ShNanA gene, resulting in 56.82 g L−1 NeuAc production. Finally, formation of the by‐product acetoin from pyruvate is blocked by deleting the alsS and alsD genes, resulting in 68.75 g L−1 NeuAc production with a molar conversion rate of 55.57% from GlcNAc. Overall, a GRAS B. subtilis strain is demonstrated as a whole‐cell biocatalyst for efficient NeuAc production.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Pathway Engineering of Bacillus subtilis for Enhanced N‐Acetylneuraminic Acid Production via Whole‐Cell Biocatalysis

Zhao

Tian

Shen

et al. 2019

Biotechnology Journal

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“… Biocatalyst Strategy Production Applications Ref. Spore based biocatalysts Spore surface display Spore coat protein Target genes N-acetyl- d -neuraminic acid Aldolase CotG nanA 4.9 g/L/h N-acetyl- d -neuraminic acid Pharmaceutical [ 97 ] β-galactosidase CotX bgaB 8.8 g/L lactulose from 200 g/L lactose and 100 g/L of fructose Pharmaceutical and dairy industry [ 98 ] CotG araA 4.3 g/L/h d -tagatose Food industry [ 99 ] CotG lacZ 8.1 g/L octyl- d -galactopyranoside Chemical industry [ 23 , 95 ] d -psicose 3-epimerase CotG Dpe 85 g/L d -allulose from 500 g/L d -fructose Pharmaceutical and food industry [ 100 ] Haloalkane Dehydrgenase CotG dhaA 1.74 ± 0.06 U/mL hydrolyzing activitiy of haloalkane dehalogenase Bioremediation [ ...…”

Section: Engineered B Subtilis Biofilms and Spores For Biocatalysismentioning

confidence: 99%

“…The biosurfactants display better performance in biotechnology applications and have less impact on the environment than conventional surfactants, due to their higher biodegradability and lower toxicity [ 21 ]. In addition, the surface display technology has demonstrated that bioactive molecules are present on the spore surface [ 22 ]. These recombinant spores have been used for enzyme immobilization that can occur irrespective of the cytoplasmic membrane permeability barrier [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials

Mohsin

Omer

Huang

et al. 2021

Synthetic and Systems Biotechnology

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Bacillus subtilis is a commonly used commercial specie with broad applications in the fields of bioengineering and biotechnology. B. subtilis is capable of producing both biofilms and spores. Biofilms are matrix-encased multicellular communities that comprise various components including exopolysaccharides, proteins, extracellular DNA, and poly-γ-glutamic acid. These biofilms resist environmental conditions such as oxidative stress and hence have applications in bioremediation technologies. Furthermore, biofilms and spores can be engineered through biotechnological techniques for environmentally-friendly and safe production of bio-products such as enzymes. The ability to withstand with harsh conditions and producing spores makes Bacillus a suitable candidate for surface display technology. In recent years, the spores of such specie are widely used as it is generally regarded as safe to use. Advances in synthetic biology have enabled the reprogramming of biofilms to improve their functions and enhance the production of value-added products. Globally, there is increased interest in the production of engineered biosensors, biocatalysts, and biomaterials. The elastic modulus and gel properties of B. subtilis biofilms have been utilized to develop living materials. This review outlines the formation of B. subtilis biofilms and spores. Biotechnological engineering processes and their increasing application in bioremediation and biocatalysis, as well as the future directions of B. subtilis biofilm engineering, are discussed. Furthermore, the ability of B. subtilis biofilms and spores to fabricate functional living materials with self-regenerating, self-regulating and environmentally responsive characteristics has been summarized. This review aims to resume advances in biological engineering of B. subtilis biofilms and spores and their applications.

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“…Transformation of α-D-glucosamine-1phosphate to N-acetyl-α-D-glucosamine-6-phosphate has been completed, and with phosphatases-catalyst to establish conversion of 6-ester. This reaction's key step is the rate limitation imposed by the chemical cleavage of phosphate and transfer within a molecule [33].…”

Section: The Enzymatic Modification Of N-acetyl-α-d-glucosamine-n-phosphatementioning

confidence: 99%

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

Senan¹

2021

J Food Chem Nanotechnol

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The enzymatic modification of glucosamine (GlcN) and its derivatives have a keen interest for researchers due to the natural abundance of its bio-sourced such as chitin. The recent progress for understanding the role of glucosamine based on various novel approaches for enzymatic synthesis in the field of medicinal chemistry and food supplements. These developments led to improve chemo-selective protection and/or deprotection of amino-sugar during chainenhancement and allow the simultaneous regioselective reaction of functionally relevant acetyl, phosphate, or sulfate groups. In this review, we give an overview of current strategies on the synthesis of therapeutically relevant glucosaminecontaining derivatives.

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Chemoenzymatic Synthesis of N -Acetyl- d -Neuraminic Acid from N -Acetyl- d -Glucosamine by Using the Spore Surface-Displayed N -Acetyl- d -Neuraminic Acid Aldolase

Cited by 19 publications

References 25 publications

Pathway Engineering of Bacillus subtilis for Enhanced N‐Acetylneuraminic Acid Production via Whole‐Cell Biocatalysis

Pathway Engineering of Bacillus subtilis for Enhanced N‐Acetylneuraminic Acid Production via Whole‐Cell Biocatalysis

Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

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