Chemoenzymatic synthesis of polypeptides in neat 1,1,1,2-tetrafluoroethane solvent

Abstract

Chemoenzymatic polypeptide synthesis offers several advantages over chemical or other biological routes, however, the use of aqueous-based media suffers from reverse hydrolysis reactions that challenge peptide chain propagation.

Facile terminal functionalization of peptides by protease-catalyzed chemoenzymatic polymerization toward synthesis of polymeric architectures consisting of peptides

“…16 So far, the molecular weight obtained by the chemoenzymatic polymerization in aqueous media has reached up to approximately 2000 Da depending on the amino acid residues that characterize the reactivity of monomers and the solubility of resulting polypeptides in water. 29 The use of water-free conditions such as solution polymerization in an organic solvent 35 or bulk polymerization 36 can improve the molecular weight of the obtained polypeptides, although the greenness of the chemoenzymatic synthesis is deteriorated. The papain-catalyzed polymerization in the presence of 1 was also applied to other amino acid esters.…”