Chemokine Class Differences in Binding to the Duffy Antigen-Erythrocyte Chemokine Receptor

Szabo, M C; Soo, Kenneth; Zlotnik, Albert; Schall, Thomas J.

doi:10.1074/jbc.270.43.25348

Cited by 137 publications

(92 citation statements)

References 12 publications

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“…Duffy antigen, also called Duffy antigen receptor for chemokines (DARC) is a typical decoy receptor that binds with angiogenic ELR þ (glutamine-leucinearginine motif in N-terminus) CXC chemokines, as well as some CC chemokines (Neote et al, 1993(Neote et al, , 1994, but not with ELR À CXC chemokines and C chemokine (Szabo et al, 1995). DARC ligands with high affinity include CXCL1 (GROa), CXCL2 (GROb), CXCL3 (GROg), CXCL4 (PF4), CXCL5 (ENA-78), CXCL7 (NAP-2), CXCL8 (IL-8), CCL2 (MCP-1, MCAF, JE), CCL5 (RANTES), CCL7 (MCP-3), CCL11 (Eotaxin), CCL13 (MCP-4), CCL14 (HCC-1) and CCL17 (TARC), etc (Comerford and Nibbs, 2005).…”

Section: Introductionmentioning

confidence: 99%

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

et al. 2006

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In addition to the role in regulating leukocyte trafficking, chemokines recently have been shown to be involved in cancer growth and metastasis. Chemokine network in tumor neovascularity may be regulated by decoy receptors. Duffy antigen receptor for chemokines (DARC) is a specific decoy receptor binding with the angiogenic CC and CXC chemokines. To investigate the effects of DARC on the tumorigenesis and the metastasis potential of human breast cancer cells, human DARC cDNA was reintroduced into the MDA-MB-231 and MDA-MB-435HM cells which have a high capability of spontaneous pulmonary metastasis. We demonstrated that DARC overexpression induced inhibition of tumorigenesis and/ or metastasis through interfering with the tumor angiogenesis in vivo. This inhibition is associated with decreasing CCL2 protein levels, and MVD and MMP-9 expression in xenograft tumors. In human breast cancer samples, we also demonstrated that low expression of the DARC protein is significantly associated with estrogen receptor (ER) status, MVD, lymph node metastasis, distant metastasis and poor survival. Our results suggest for the first time that DARC is a negative regulator of growth in breast cancer, mainly by sequestration of angiogenic chemokines and subsequent inhibition of tumor neovascularity.

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Section: Introductionmentioning

confidence: 99%

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

et al. 2006

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“…In the early 1990s it was recognised that this molecule was a seven transmembrane spanning receptor and later that it bound, with high affinity, inflammatory CC and CXC chemokines [24][25][26][27][28]. It is, thus far, the only mammalian chemokine receptor capable of binding ligands from more than one chemokine subfamily (Table 1).…”

Section: The Darcmentioning

confidence: 99%

D6 and the atypical chemokine receptor family: Novel regulators of immune and inflammatory processes

2009

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Chemokines are key regulators of leukocyte migration and play important roles in a number of physiological and pathological immune and inflammatory contexts. In addition to the classical signalling chemokine receptors there has emerged, recently, a new subclass of atypical chemokine receptors. This subfamily is characterised by an apparent lack of signalling and, in some cases, by an ability to internalise and degrade chemokine ligands. This review describes the family of atypical chemokine receptors with particular emphasis on the D6 receptor. The in vitro and in vivo biology of D6 is described, which indicates that D6 is active as a scavenger of inflammatory CC-chemokines and appears to play essential roles in the regulation of inflammatory responses.Key words: Chemokines . Immune regulation . Inflammation IntroductionThe movement of leukocytes during immune and inflammatory responses is a carefully orchestrated process that ensures coordinated cellular migration in response to physiological and pathological cues. Prominent amongst the regulators of leukocyte movement are the members of the chemokine family [1]. Chemokines are small proteins (8-12 kDa) and membership of the chemokine family is defined on the basis of the presence of variations on a conserved cysteine motif in the mature section of the proteins. Chemokines can be assigned to one of four subfamilies according to the specific nature of this motif. The majority of chemokines have four cysteines with 28 chemokines being assigned to the CC family (so-called because of the juxtaposition of the first two cysteine residues) and 16 being assigned to the CXC family (which possess a single variable amino acid between the first two cysteines). The two smaller subfamilies are represented by single members and are the CX3C family (three amino acids between the first two cyteines) and the XC family, which lacks the first and third cyteines seen in the other family members. Much confusion arose in the mid-1990s due to the complex and variable nomenclature used to refer to chemokines. For this reason a systematic nomenclature system has been adopted, which refers to the chemokines as ligands of each of the subfamilies and numbers them according to when their sequences were first deposited in the Genbank databases [2]. Thus, CCL1 is the first CC chemokine to be identified and CCL28 the most recent. Chemokines and their roles in leukocyte migrationFunctionally, chemokines are known to be pleiotropic. However, their main role is in the regulation of leukocyte migration. In this context we can define chemokines as being either homeostatic/ constitutive or inflammatory according to the contexts in which they function [2,3]. Homeostatic chemokinesThe homeostatic chemokines are important for the regulation of basal leukocyte trafficking and regulate processes such as the Mini-Reviewtissue-specific migration of T cells and the homing of DC to draining lymph nodes [4][5][6]. In combination with adhesion molecules, the homeostatic chemokines can form part of a very spec...

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“…These receptors bind to chemokines and remove them from the inflammatory milieu (134,406). DARC has been shown to bind to the members of both CC and CXC chemokine subclasses (668). Interestingly DARC binds to angiogenic (ELR + ) CXC chemokines but not to angiostatic (ELR -) CXC chemokines.…”

Section: Chemokine Receptorsmentioning

confidence: 99%

The role of CCR5 in vaccinia virus pathogenesis

Rahbar

Fish

2009

Cytokine

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Viral appropriation of chemokine receptors is an effective way to prevent a host immune response against the invading virus. Many viruses, including poxviruses, subvert the host immune response by encoding several chemokine receptor homologues, capable of binding to and thereby precluding chemokines from activating their cognate cell surface receptors. All poxviruses employ strategies to modulate chemokine activity, including virus-encoded chemokine-binding proteins, receptor homologues and ligand mimics. The potential for the involvement of certain chemokine receptors in poxviral infection was suggested in studies utilizing the rabbit poxvirus, myxoma. Specifically, CCR5 was implicated in mediating cell target susceptibility to infection. Our data suggest virus-CCR5 interactions may lead to the selective activation of distinct signaling pathways that are advantageous for the virus.VACV, a member of the poxvirus family, produces two structurally distinct forms of virions, the intracellular mature virus (IMV) and the extracellular enveloped virus (EEV), for which the immediate events following cell entry are illiii defined. Using confocal microscopy, we provided evidence that IMV and EEV enter both permissive and non-permissive cells, and that introduction of CCR5 into non-permissive cells -mouse fibroblasts and human PM1 T cells -renders them permissive for VACV replication. We showed that virus activation of CCR5 leads to the selective activation of distinct signaling pathways that are advantageous for the virus. We demonstrated that VACV infection in permissive cells is inhibited by siRNA knockdown of cell surface

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Chemokine Class Differences in Binding to the Duffy Antigen-Erythrocyte Chemokine Receptor

Cited by 137 publications

References 12 publications

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

D6 and the atypical chemokine receptor family: Novel regulators of immune and inflammatory processes

The role of CCR5 in vaccinia virus pathogenesis

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