Chemokine expression in human 3-dimensional cultured epidermis exposed to PM2.5 collected by cyclonic separation

Leaf extracts from Camellia sinensis and Argania spinosa suppress oxidative stress and chemokine release in human 3-dimensional cultured epidermis exposed to PM2.5 collected with cyclonic separation

“…We previously reported that exposure to PM2.5, which was collected by a cyclonic separation method directly from the air, increased the expression of chemokines CXCL1 and IL-8 in human 3-dimensional cultured epidermis and that these chemokines were released from the epidermis and induced neutrophil chemotaxis (Kono et al, 2022). Because chemokine release occurs downstream of oxidative stress induced by PM2.5 exposure (Kono et al, 2022), antioxidants could suppress chemokine release and subsequent neutrophilic inflammation.…”

“…We previously reported that exposure to PM2.5, which was collected by a cyclonic separation method directly from the air, increased the expression of chemokines CXCL1 and IL-8 in human 3-dimensional cultured epidermis and that these chemokines were released from the epidermis and induced neutrophil chemotaxis (Kono et al, 2022). Because chemokine release occurs downstream of oxidative stress induced by PM2.5 exposure (Kono et al, 2022), antioxidants could suppress chemokine release and subsequent neutrophilic inflammation. We screened 42 kinds of plant extracts by DPPH and ABTS radical scavenging activities, and we determined that leaf extracts from Camellia sinensis showed the highest radical scavenging activity and that the Argania spinosa leaf extract showed the second highest radical scavenging activity (Fig.…”

“…We previously reported that chemokine expression and subsequent neutrophil chemotaxis occurred after PM2.5 exposure in a human 3-dimensional cultured epidermis and that oxidative stress can be involved in this chemokine expression (Kono et al, 2022). Therefore, antioxidants could preserve skin health from PM2.5 exposure via suppression of oxidative stress and subsequent neutrophilic inflammation.…”

A simple air–liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation

“…Liao et al reported that the cholesterol levels of a 3D epidermis model increased after treating it with PM2.5 (Liao et al, 2019). It was also shown that PM2.5 induced chemokine release, followed by neutrophil activation, which can cause an unregulated inflammatory reaction, in a cultured human 3D epidermis model (Kono et al, 2023).…”

Efficient V-Shaped Substrate for Surface and Volume Enhanced Raman Spectroscopic Analysis of Bioaerosol: Prevention from Potential Health Risk

“…The profound impact of bioaerosols on public health and safety is a major concern, − as they essentially contain pathogenic active components that are adverse to the health of people all over the world considering that they can migrate and be transported everywhere through the persistent moving air mass in a wide scale. Especially when the aerodynamic diameter is less than 5 μm, the microorganism bioaerosols can readily enter the human body, , which further raises a range of allergic, pathogenic, and reactive diseases such as COVID-19 (coronavirus), varicella (herpes zoster virus), tuberculosis ( Mycobacterium tuberculosis ), etc. Therefore, developing efficient and accurate detection and analysis of bioaerosols is very important for evaluating the potential risk and guaranteeing the safety of human and other organisms’ lives and health. , Considerable numbers of detection techniques for bioaerosols have been developed over many past generations. ,− Traditional detection techniques mainly rely upon culture methods due to their rare abundance, and this type of technique is routinely time-consuming and only 0.1–15% of microorganisms in the air can be cultured. , In light of this reasoning, culture-independent detection techniques receive much interest and have been rapidly developed in recent decades, such as the polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), etc .…”