Chemokine Homeostasis in Healthy Volunteers and during Pancreatic and Colorectal Tumor Growth in Murine Models

Svirshchevskaya, E. V.; Konovalova, M. V.; Snezhkov, E. V.; Полтавцева, Р. А.; Akopov, S. B.

doi:10.3390/cimb44100339

Cited by 2 publications

(1 citation statement)

References 65 publications

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“…The effects of chemokines have been found in: the stimulation and migration of various leukocyte populations, anti-infectious immunity, hemo-and lymphopoiesis, embryogenesis, organogenesis, the regulation of apoptosis and angiogenesis. Some of these processes are significantly involved in cancer progression and metastasis, which encouraged our research team to focus on the role of these proteins in the development of colorectal cancer, to investigate how their concentrations change in the course of this disease and to determine whether there are dependencies between their concentrations [12][13][14][15][16][17].…”

Section: Discussionmentioning

confidence: 99%

A Novel Approach to Staging and Detection of Colorectal Cancer in Early Stages

Zajkowska

Mroczko

2023

JCM

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Colorectal cancer (CRC) is a significant problem affecting patients all over the world. Since it is the fourth most common cause of cancer-related deaths, many scientists aim to expand their knowledge on the detection in early stages and treatment of this disease. Chemokines, as protein parameters involved in many processes accompanying the development of cancer, constitute a group of potential biomarkers that could also be useful in the detection of CRC. For this purpose, our research team used the results of thirteen parameters (nine chemokines, one chemokine receptor and three comparative markers, i.e., CEA, CA19-9 and CRP) to calculate one hundred and fifty indexes. Moreover, for the first time, the relationship between these parameters during the ongoing cancer process and in comparison to a control group are presented. As a result of statistical analyses using patients’ clinical data and the obtained indexes, it was established that several of the indexes have a diagnostic utility that is much higher than the tumor marker that is currently the most commonly used (CEA) currently. Furthermore, two of the indexes (CXCL14/CEA and CXCL16/CEA) showed not only extremely high usefulness in the detection of CRC in its early stages, but also the ability to determine whether the stage is low (stage I and II) or high (stage III and IV).

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Section: Discussionmentioning

confidence: 99%

A Novel Approach to Staging and Detection of Colorectal Cancer in Early Stages

Zajkowska

Mroczko

2023

JCM

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Pan02 pancreatic tumor models carrying the GFP marker in mice

Akopov,

Snezhkov,

Konovalova

et al. 2024

Med. immunol.

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Animal tumor models are used for preclinical studies of drugs and cancer therapy. The aim of this work was to analyze the growth of murine pancreatic tumor cells Pan02, carrying GFP marker, injected subcutaneously (s. c.), intraperitoneally (i. p.) or orthotopically into the pancreas (ortho) of C57BL/6 mice. Mice were injected with 2 × 105 cells: s. c. in the right flank; i. p. with a syringe into the abdominal cavity, or ortho surgically under the pancreas capsule. The weight of mice was determined in the dynamics of tumor growth, and blood serum was taken to analyze the antibody response to the GFP reference protein. At the 2nd and 4th weeks of tumor growth, some mice were slaughtered and the expression of GFP by the tumor cells, as well as the composition of the immune cells in the tumor, were analyzed by flow cytometry and confocal microscopy. It was shown that with the different localization, the pancreatic tumors grew at different rates and lethality. When the tumor was injected i. p., mice lost weight with rapid tumor growth. In the ortho model, the mice increased their weight. Mortality in the s. c. and i. p. groups was comparable. In the s. c. model, the tumor grew slowly to a volume of 200-400 mm3 and stopped growing. There was no mortality in this group during the follow-up period (2 months). The same antibody response to GFP was formed with all injection schemes. The subpopulation composition of immune cells varied greatly in the different models of tumor cell administration. Regardless of the type of immune response, Pan02-GFP cells rapidly suppressed GFP gene expression in vivo. The data obtained showed that murine pancreatic tumor Pan02 is immunogenic and causes the formation of an adaptive immune response. Regardless of the presence or absence of an immune response and elimination of GFP+ cells, the tumor continued to grow in the i. p. and ortho models, but not in the s. c. one, and caused the death of mice. When conducting preclinical studies, it is necessary to use several ways of tumor cell injection to obtain a more objective result.

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Chemokine Homeostasis in Healthy Volunteers and during Pancreatic and Colorectal Tumor Growth in Murine Models

Cited by 2 publications

References 65 publications

A Novel Approach to Staging and Detection of Colorectal Cancer in Early Stages

A Novel Approach to Staging and Detection of Colorectal Cancer in Early Stages

Pan02 pancreatic tumor models carrying the GFP marker in mice

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