Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates

Clay, Candice C.; Rodrigues, Denise; Brignolo, Laurie; Spinner, Abbie; Tarara, Ross P.; Plopper, Charles G.; Leutenegger, Christian M.; Esser, Ursula

doi:10.1016/j.jim.2004.06.019

Cited by 18 publications

(22 citation statements)

References 53 publications

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“…Relative transcript levels for homeostatic chemokine CCL19 and proinflammatory chemokine CXCL9 were determined for secondary lymphoid organs and gut tissues in a group of uninfected animals (numbers 30880, 30892, and 30893) and acutely SIV mac251 -infected animals (numbers 30019, 29677, and 32222) at necropsy time point (day 14 p.i. in the SIV-infected group), according to previously established procedures (7). Transcript levels were normalized based on GAPDH transcription; for each chemokine, values were calibrated based on the tissues expressing the lowest levels in the SIV-uninfected group.…”

Section: Resultsmentioning

confidence: 99%

“…Autologous lymphocyte transfer and necropsy tissue collection. Lymphocytes from the acutely SIV-infected macaques were isolated at day 12 p.i., according to previously established procedures (7). In brief, animals were anesthetized and sequentially bled, and lymphocytes were purified by density gradient centrifugation on Ficoll-Hypaque (Amersham Biosciences, Piscataway, NJ (7).…”

Section: Methodsmentioning

confidence: 99%

“…Lymphocytes from the acutely SIV-infected macaques were isolated at day 12 p.i., according to previously established procedures (7). In brief, animals were anesthetized and sequentially bled, and lymphocytes were purified by density gradient centrifugation on Ficoll-Hypaque (Amersham Biosciences, Piscataway, NJ (7). At the time of necropsy, maximal blood volumes were collected, and all six animals were whole-body saline perfused prior to tissue collection into RPMI (Invitrogen, Carlsbad, CA) and formalin.…”

Section: Methodsmentioning

confidence: 99%

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Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV _mac251 -Infected and Uninfected Macaques

Clay

Rodrigues

Harvey

et al. 2005

J Virol

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To define the possible impact of T-lymphocyte trafficking parameters on simian immunodeficiency virus (SIV) pathogenesis, we examined migratory profiles of carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled T lymphocytes in acutely SIV mac251 -infected and uninfected macaques within 48 h after autologous transfer. Despite significant upregulation of homeostatic chemokine CCL19/macrophage inflammatory protein 3␤ and proinflammatory chemokine CXCL9/monokine induced by gamma interferon in secondary lymphoid tissue in SIV infection, no differences in CFSE ؉ T-lymphocyte frequencies or cell compartmentalization in lymph nodes were identified between animal groups. By contrast, a higher frequency of CFSE ؉ T lymphocytes in the small intestine was detected in acute SIV infection. This result correlated with increased numbers of gut CD4 T lymphocytes expressing chemokine receptors CCR9, CCR7, and CXCR3 and high levels of their respective chemokine ligands in the small intestine. The changes in trafficking parameters in SIV-infected macaques occurred concomitantly with acute gut CD4 T-lymphocyte depletion. Here, we present the first in vivo T-lymphocyte trafficking study in SIV infection and a novel approach to delineate T-lymphocyte recruitment into tissues in the nonhuman primate animal model for AIDS. Such studies are likely to provide unique insights into T-lymphocyte sequestration in distinct tissue compartments and possible mechanisms of CD4 T-lymphocyte depletion and immune dysfunction in simian AIDS.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV _mac251 -Infected and Uninfected Macaques

Clay

Rodrigues

Harvey

et al. 2005

J Virol

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“…on May 9, 2018. by guest www.bloodjournal.org From (ie, jejunum for CCL19, skin for CCL20, and lung for both CCL21 and CCL25). 27 …”

Section: Chemokine Quantificationmentioning

confidence: 99%

Injection of glycosylated recombinant simian IL-7 provokes rapid and massive T-cell homing in rhesus macaques

Beq

Rozlan

Gautier

et al. 2009

Blood

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Interleukin-7 (IL-7), the principal cytokine implicated in thymopoiesis and peripheral T-cell homeostasis, is presently under evaluation in human diseases characterized by persistent lymphopenia. Unexpectedly, before the eventual IL-7-driven T-cell expansion, all treated patients showed a profound T-cell depletion 24 hours after injection. The current study uses the rhesus macaque model to investigate the mechanisms involved in this IL-7-induced T-cell depletion. We identify a new critical function of IL-7 that induces massive and rapid T-cell migration from the blood into various organs, including lymph nodes, parts of the intestine, and the skin. This homing process was initiated after the induction of chemokine receptor expression by circulating T cells and the production of corresponding chemokines in target organs. Finally, we demonstrate that the IL-7-induced cell cycling is initiated within these organs before T cells migrate back into the bloodstream, indicating that T-cell homing is required for in vivo IL-7 function. (Blood. 2009;114:816-825)

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“…Here we utilized a novel adoptive transfer approach in primates (11) to track fluorescein dye-labeled blood monocytes from the peripheral circulation into the CNS in SIV-infected rhesus macaques. We observed brain trafficking of fluoresceinlabeled monocytes within a 48-h in vivo migratory period in acute SIV infection, which coincided with the presence of the virus in the CNS.…”

mentioning

confidence: 99%

Neuroinvasion of Fluorescein-Positive Monocytes in Acute Simian Immunodeficiency Virus Infection

Clay

Rodrigues

et al. 2007

J Virol

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Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates

Cited by 18 publications

References 53 publications

Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV _mac251 -Infected and Uninfected Macaques

Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV _mac251 -Infected and Uninfected Macaques

Injection of glycosylated recombinant simian IL-7 provokes rapid and massive T-cell homing in rhesus macaques

Neuroinvasion of Fluorescein-Positive Monocytes in Acute Simian Immunodeficiency Virus Infection

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Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates

Cited by 18 publications

References 53 publications

Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV mac251 -Infected and Uninfected Macaques

Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV mac251 -Infected and Uninfected Macaques

Injection of glycosylated recombinant simian IL-7 provokes rapid and massive T-cell homing in rhesus macaques

Neuroinvasion of Fluorescein-Positive Monocytes in Acute Simian Immunodeficiency Virus Infection

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Product

Resources

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Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV _mac251 -Infected and Uninfected Macaques

Distinct Chemokine Triggers and In Vivo Migratory Paths of Fluorescein Dye-Labeled T Lymphocytes in Acutely Simian Immunodeficiency Virus SIV _mac251 -Infected and Uninfected Macaques