Chemokine Receptors and Phagocyte Biology in Zebrafish

Sommer, Frida; Torraca, Vincenzo; Meijer, Annemarie H.

doi:10.3389/fimmu.2020.00325

Cited by 46 publications

(23 citation statements)

References 109 publications

(305 reference statements)

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“…However, this response generates pathological consequences that lead to inflammatory tissue damage, metaplasia, and a shift in homeostatic set points (Medzhitov, 2008). The zebrafish (Danio rerio), as a model organism, has been widely used in scientific research, including immunology (Rosowski, 2020;Sommer et al, 2020). Zebrafish larvae are suitable for investigating the innate immune response in vertebrates because their adaptive immune system matures in morphology and function 4-6 weeks after fertilization, while only innate immune response exists in larvae.…”

Section: Discussionmentioning

confidence: 99%

Phyllolobium chinense Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway

Fan

Peng

et al. 2020

Front. Pharmacol.

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Background: M1 macrophage plays an important role in inflammatory reaction. In this study, potential anti-inflammatory effect of Phyllolobium chinense Fisch flavonoids (PCFF) was assessed via Zebrafish acute inflammation model in vivo and LPS-induced proinflammatory M1 macrophage model in vitro. Methods:The quality control of P. chinense Fisch flavonoids (PCFF) was analyzed by HPLC. Anti-inflammatory effect of PCFF on the acute injured zebrafish was evaluated by the migration of fluorescence labeled macrophages and neutrophils, and the gene expression of inflammatory factors. In addition, the anti-inflammatory mechanism of PCFF was investigated by the related gene expression and related signaling pathway regulation of pro-inflammatory mediators in LPS-induced pro-inflammatory M1 RAW264.7 macrophage.Results: P. chinense Fisch flavonoids (PCFF) markedly suppressed macrophage and neutrophil migration and iNOS gene expression in acute injured zebrafish with tail-cutting. PCFF significantly inhibited NO overproduction and iNOS gene overexpression in LPSsitimulated pro-inflammatory M1 RAW264.7 macrophages. What's more, PCFF could evidently decrease p65 protein production, but had no effect on the production of P38, JNK and ERK1/2 proteins. Conclusion: P. chinense Fisch flavonoids (PCFF) have a remarkable inhibitory effect on the inflammatory response in acute injured zebrafish and LPS-stimulated M1 RAW264.7 macrophage. The pharmacological mechanism may be related to the regulation of NO overproduction and the inhibition of NF-kB/iNOS signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Phyllolobium chinense Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway

Fan

Peng

et al. 2020

Front. Pharmacol.

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“…The migration of phagocytes is orchestrated by chemokine signaling axes, which are generally well conserved between humans and zebrafish (Sommer et al., 2020b ). The zebrafish homologs of the human CCR2 chemokine receptor and its ligand CCL2 were shown to play an important role in establishing the initial infection.…”

Section: New Insights Into Phagocyte Migration and Dissemination Of M...mentioning

confidence: 99%

A fresh look at mycobacterial pathogenicity with the zebrafish host model

Varela

Meijer

2021

Molecular Microbiology

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Mycobacteria are the causative agents of some of the most serious human infectious diseases, including tuberculosis (TB), leprosy, and various other lung, skin, and disseminated infections (Kilinc et al., 2021). Pathogenic mycobacteria have developed highly effective virulence mechanisms to subvert the immune defenses of their host. In particular, mycobacterial pathogens are able to establish a replication niche inside macrophages and subsequently manipulate these innate immune cells to act as transport vehicles to invade host tissues (Houben et al., 2006). Following tissue invasion, inflammatory lesions, known as tuberculous granulomas, are

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“…The inflammatory exudate and the blood flow are able to produce a local tumour in the affected area, where all mediators previously released trigger the production of immunoglobulins, kinins, acute‐phase reactants and coagulation factors while generating complement activation (Bordés Gonzalez et al., 1994; Figure 3). Mammals and zebrafish neutrophils recruited at the inflammation place shortly secrete chemokines that activate tissue‐resident cells and recruit more leucocytes to the damaged area, amplifying inflammation (Kolaczkowska & Kubes, 2013; Sommer et al., 2020). Several studies in fish, like those developed by our research team in the marine teleost gilthead seabream ( Sparus aurata L.), point to the acidophilic granulocytes as the functional counterparts of the neutrophils of higher vertebrates (Meseguer et al., 1994; Morcillo et al., 2015; Sepulcre et al., 2002).…”

Section: Stages Of Inflammation—the Backgroundmentioning

confidence: 99%

Review of inflammation in fish and value of the zebrafish model

Campos-Sánchez

Esteban

2020

Journal of Fish Diseases

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Inflammation is a crucial step in the development of chronic diseases in humans. Understanding the inflammation environment and its intrinsic mechanisms when it is produced by harmful stimuli may be a key element in the development of human disease diagnosis. In recent decades, zebrafish (Danio rerio) have been widely used in research, due to their exceptional characteristics, as a model of various human diseases. Interestingly, the mediators released during the inflammatory response of both the immune system and nervous system, after its integration in the hypothalamus, could also facilitate the detection of injury through the register of behavioural changes in the fish. Although there are many studies that give well‐defined information separately on such elements as the recruitment of cells, the release of pro‐ and anti‐inflammatory mediators or the type of neurotransmitters released against different triggers, to the best of our knowledge there are no reviews that put all this knowledge together. In the present review, the main available information on inflammation in zebrafish is presented in order to facilitate knowledge about this important process of innate immunity, as well as the stress responses and behavioural changes derived from it.

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Chemokine Receptors and Phagocyte Biology in Zebrafish

Cited by 46 publications

References 109 publications

Phyllolobium chinense Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway

Phyllolobium chinense Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway

A fresh look at mycobacterial pathogenicity with the zebrafish host model

Review of inflammation in fish and value of the zebrafish model

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