Chemokine transcription in rainbow trout (Oncorhynchus mykiss) is differently modulated in response to viral hemorrhagic septicaemia virus (VHSV) or infectious pancreatic necrosis virus (IPNV)

Montero, Jana; Chaves-Pozo, Elena; Cuesta, Alberto; Tafalla, Carolina

doi:10.1016/j.fsi.2009.08.003

Cited by 44 publications

(21 citation statements)

References 39 publications

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“…It has been shown previously that recombinant trout CXCL8_L1 enhances migration of neutrophils and macrophages (Montero et al, 2009;Harun et al, 2008), Fig. 2.…”

Section: Cxcl11_l1 Cxcl_f4 and Cxcl_f5 Are Involved In Regulating MImentioning

confidence: 73%

“…Differential responses of CXC chemokines to PRR ligands are reported. For example, the CXCL13 homologue was shown to be activated by LPS but not polyI:C (Kim et al, 2007) and a trout CXCL9-11 homologue was induced during viral infection and by polyI:C, a known ligand of Toll like receptor 3 (Chaves-Pozo et al, 2010; Montero et al, 2009). Some cytokines are also potent inducers of CXC chemokine expression, as seen with trout interferon gamma (IFN-c) and tumor necrosis factor (TNF)-a that induce CXCL9-11 expression, which is clearly an IFN-c inducible protein (cIP) Chen et al, 2010;Zou et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish

Chen

Wang

et al. 2013

Developmental & Comparative Immunology

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“…It has been shown previously that recombinant trout CXCL8_L1 enhances migration of neutrophils and macrophages (Montero et al, 2009;Harun et al, 2008), Fig. 2.…”

Section: Cxcl11_l1 Cxcl_f4 and Cxcl_f5 Are Involved In Regulating MImentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish

Chen

Wang

et al. 2013

Developmental & Comparative Immunology

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“…We have previously established that at these days postinfection, VHSV induces the transcription of several chemokine genes in both gills and head kidney (28,29). In some fish, the number of mem IgD cells was also assessed.…”

Section: Effect Of Vhsv Infection On the Distribution Of Ccr7 + Cellsmentioning

confidence: 99%

CCR7 Is Mainly Expressed in Teleost Gills, Where It Defines an IgD+IgM− B Lymphocyte Subset

Castro

Bromage

Abós

et al. 2014

The Journal of Immunology

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Chemokine receptor CCR7, the receptor for both CCL19 and CCL21 chemokines, regulates the recruitment and clustering of circulating leukocytes to secondary lymphoid tissues, such as lymph nodes and Peyer's patches. Even though teleost fish do not have either of these secondary lymphoid structures, we have recently reported a homolog to CCR7 in rainbow trout (Oncorhynchus mykiss). In the present work, we have studied the distribution of leukocytes bearing extracellular CCR7 in naive adult tissues by flow cytometry, observing that among the different leukocyte populations, the highest numbers of cells with membrane (mem)CCR7 were recorded in the gill (7.5 ± 2% CCR7+ cells). In comparison, head kidney, spleen, thymus, intestine, and peripheral blood possessed <5% CCR7+ cells. When CCR7 was studied at early developmental stages, we detected a progressive increase in gene expression and protein CCR7 levels in the gills throughout development. Surprisingly, the majority of the CCR7+ cells in the gills were not myeloid cells and did not express membrane CD8, IgM, nor IgT, but expressed IgD on the cell surface. In fact, most IgD+ cells in the gills expressed CCR7. Intriguingly, the IgD+CCR7+ population did not coexpress memIgM. Finally, when trout were bath challenged with viral hemorrhagic septicemia virus, the number of CCR7+ cells significantly decreased in the gills while significantly increased in head kidney. These results provide evidence of the presence of a novel memIgD+memIgM− B lymphocyte subset in trout that expresses memCCR7 and responds to viral infections. Similarities with IgD+IgM− subsets in mammals are discussed.

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“…As this first screening revealed an important effect of VHSV on CK12 regulation of transcription, we also included the other rainbow trout chemokines belonging to the CCL19/21/25 group, CK9 and CK10. Out of the 12 genes studies, VHSV was capable of significantly upregulating the levels of expression of only CK10 and CK12 at day 3 postinfection and of only CK10 at day 6 postinfection, revealing a very specific response, as VHSV had been proved to significantly upregulate the transcription of many of the other chemokine genes in other organs such as spleen or head kidney (38). No significant downregulation was observed.…”

Section: Resultsmentioning

confidence: 96%

“…Since the chemokine induction pattern in response to the virus at the fin bases was much more specific than what we had previously observed in immune organs (38), we studied in parallel the chemokine response to another mucosal tissue, the gills. Previous studies had revealed that although some viral particles could be found in the gills upon bath infection, this is not the main site of viral entry for fish rhabdoviruses (17), and viral replication can be detected in only a few specific cells (4).…”

Section: Resultsmentioning

confidence: 99%

Specific Regulation of the Chemokine Response to Viral Hemorrhagic Septicemia Virus at the Entry Site

Montero¹,

Garcia²,

Ordás³

et al. 2011

J Virol

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The fin bases constitute the main portal of rhabdovirus entry into rainbow trout (Oncorhynchus mykiss), and replication in this first site strongly conditions the outcome of the infection. In this context, we studied the chemokine response elicited in this area in response to viral hemorrhagic septicemia virus (VHSV), a rhabdovirus. Among all the rainbow trout chemokine genes studied, only the transcription levels of CK10 and CK12 were significantly upregulated in response to VHSV. As the virus had previously been shown to elicit a much stronger chemokine response in internal organs, we compared the effect of VHSV on the gills, another mucosal site which does not constitute the main site of viral entry or rhabdoviral replication. In this case, a significantly stronger chemokine response was triggered, with CK1, CK3, CK9, and CK11 being upregulated in response to VHSV and CK10 and CK12 being down-modulated by the virus. We then conducted further experiments to understand how these different chemokine responses of mucosal tissues could correlate with their capacity to support VHSV replication. No viral replication was detected in the gills, while at the fin bases, only the skin and the muscle were actively supporting viral replication. Within the skin, viral replication took place in the dermis, while viral replication was blocked within epidermal cells at some point before protein translation. The different susceptibilities of the different skin layers to VHSV correlated with the effect that VHSV has on their capacity to secrete chemotactic factors. Altogether, these results suggest a VHSV interference mechanism on the early chemokine response at its active replication sites within mucosal tissues, a possible key process that may facilitate viral entry.

show abstract

Chemokine transcription in rainbow trout (Oncorhynchus mykiss) is differently modulated in response to viral hemorrhagic septicaemia virus (VHSV) or infectious pancreatic necrosis virus (IPNV)

Cited by 44 publications

References 39 publications

Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish

Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish

CCR7 Is Mainly Expressed in Teleost Gills, Where It Defines an IgD+IgM− B Lymphocyte Subset

Specific Regulation of the Chemokine Response to Viral Hemorrhagic Septicemia Virus at the Entry Site

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