Chemokines enhance immunity by guiding naive CD8+ T cells to sites of CD4+ T cell–dendritic cell interaction

Castellino, Flora; Huang, Alex Y.; Altan‐Bonnet, Grégoire; Stoll, Sabine; Scheinecker, Clemens; Germain, Ronald N.

doi:10.1038/nature04651

Cited by 755 publications

(688 citation statements)

References 35 publications

Supporting

Mentioning

631

Contrasting

Unclassified

Order By: Relevance

“…In vivo, a highly developed lymphoid architecture along with defined migration pathways ascertain the encounter of naïve CD4 cells with the proper APC [30][31][32]. For CD8 cells, chemokines have recently been shown to play a critical role in attracting the few antigen-specific naïve cells to the site of DC-CD4 cell interaction in lymph nodes [33]. The impact of the APC type on memory CD4 cell function upon antigen re-encounter is less well defined.…”

Section: Discussionmentioning

confidence: 99%

The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types

Ott¹,

Tary‐Lehmann²,

Lehmann³

2007

Clinical Immunology

View full text Add to dashboard Cite

It is unknown to what extent the heterogeneity of antigen presenting cells (APC) influences the IFN-γ response of CD4 memory cells. We restimulated DO11.10 T cell receptor (TCR)-transgenic cells and wild-type CD4 memory cells with OVA-peptide 323 -339 presented on purified dendritic cells (DC), macrophages, and B cells. Using IFN-γ ELISPOT assays we measured the number of cytokine producing T cells and the amount of cytokine produced by individual T cells at different time points after antigen encounter. The data showed that, when CD4 cells recognized antigen on DC, the induction of cytokine production was accelerated compared to macrophages and B cells. In contrast, the per-cell cytokine productivity was independent of the type of APC by which the T cells were restimulated. Moreover, the peptide concentration required for CD4 cell activation was comparable for the different APC. The data suggest that DC induce cytokine production in memory cells with accelerated activation kinetics, whereas 24 h of antigen stimulation on DC, macrophages, and B cells results in comparable levels of T cell activation. These data have implications for the understanding of T cell memory responses when T cells re-encounter antigen on different APC as well as for the monitoring of memory T cell responses ex vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types

Ott¹,

Tary‐Lehmann²,

Lehmann³

2007

Clinical Immunology

View full text Add to dashboard Cite

show abstract

“…CCR5 up-regulation by CD8 + T cells allows their attraction to sites where the cognate chemokines are produced (Castellino et al 2006). Partial blockade of CCR5 in T. cruziinfected mice decreased myocarditis without hampering the control of parasite growth ).…”

Section: Discussionmentioning

confidence: 99%

TNF/TNFR1 signaling up-regulates CCR5 expression by CD8+ T lymphocytes and promotes heart tissue damage during Trypanosoma cruzi infection: beneficial effects of TNF-α blockade

Kroll-Palhares

Silvério

Silva

et al. 2008

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…This difference might be caused by the levels of some cytokines in tumor tissues and malignant fluids. Although many studies have characterized the changes of immune microenvironment through recruitment of leukocytes induced by chemokines, [35][36][37][38][39] it could be also important to elucidate the relation between expansion of T cell clonotypes and expression of chemokines.…”

Section: Discussionmentioning

confidence: 99%

Characterization of T cell repertoire of blood, tumor, and ascites in ovarian cancer patients using next generation sequencing

et al. 2015

View full text Add to dashboard Cite

Tumor-infiltrating lymphocytes (TILs) play an important role in regulating the host immune response and are one of key factors in defining tumor microenvironment. Some studies have indicated that T cell infiltration in malignant ascites is associated with clinical outcome, but few studies have performed detailed characterization of T cell diversity or clonality in malignant effusions. We have applied a next generation sequencing method to characterize T cell repertoire of a set of primary cancers, ascites, and blood from 12 ovarian cancer patients and also analyzed the T cell subtype populations in malignant fluids from 3 ovarian cancer patients. We observed enrichment of certain T cells in tumors and ascites, but most of the enriched T cell receptor (TCR) sequences in tumors and ascites were not common. Moreover, we analyzed TCR sequences of T cell subtypes (CD4 C , CD8 C , and regulatory T cells) isolated from malignant effusions and also found clonal expansion of certain T cell populations, but the TCR sequences were almost mutually exclusive among the three subgroups. Although functional studies of clonally expanded T cell populations are definitely required, our approach offers a detailed characterization of T cell immune microenvironment in tumors and ascites that might differently affect antitumor immune response.

show abstract

Chemokines enhance immunity by guiding naive CD8+ T cells to sites of CD4+ T cell–dendritic cell interaction

Cited by 755 publications

References 35 publications

The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types

The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types

TNF/TNFR1 signaling up-regulates CCR5 expression by CD8+ T lymphocytes and promotes heart tissue damage during Trypanosoma cruzi infection: beneficial effects of TNF-α blockade

Characterization of T cell repertoire of blood, tumor, and ascites in ovarian cancer patients using next generation sequencing

Contact Info

Product

Resources

About