Chemometric deconvolution of gas chromatographic unresolved conjugated linoleic acid isomers triplet in milk samples

Blaško, Jaroslav; Kubinec, Róbert; Ostrovský, Ivan; Pavlíková, Eva; Krupčík, J.; Soják, Ladislav

doi:10.1016/j.chroma.2008.11.019

Cited by 19 publications

(10 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The average relative standard deviation of the analyzed FAME with the content > 0.5 g/100 g was 1.1%, for the whole analytical procedure and 5 replicate samples. The CLA content in analyzed milk samples represents the total content of gas chromatographic unseparated triplet of cis-9,trans-11 + trans-7,cis-9 + trans-8,cis-10 CLA isomers with prevailing (about 90%) content of cis-9,trans-11 CLA (Blasko et al 2009). …”

Section: Methodsmentioning

confidence: 99%

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Soják¹,

Blaško²,

Kubinec³

et al. 2015

CZECH J ANIM SCI

View full text Add to dashboard Cite

ABSTRACT:The effects of adding sunflower and linseed oils to a standard winter ration with a lower concentrate content on the milk fatty acid composition in lactating ewes were investigated. Eighteen dairy ewes randomly chosen from the ewe flock were divided into three groups: the first group was fed a winter ration, the second one a winter ration supplemented with sunflower oil, and the third a winter ration supplemented with linseed oil for a period of 12 days. In the treatment groups, the concentrate was partially replaced by 3.0 g/100 g sunflower or linseed oils. Milk samples were taken daily from morning milking for the analysis of fatty acid composition to determine their temporal daily variations. The responses to sunflower oil compared with linseed oil addition after the end of experiment were slightly higher for conjugated linoleic acid (CLA) (P < 0.05), linoleic acid (P < 0.001), trans-10 18:1 (P < 0.001), 6:0 to 16:0 (P ≈ 0.05 -P < 0.001), whereas a higher content of α-linolenic acid (ALA) (3-fold) (P < 0.001), oleic acid (P < 0.001), and 18:0 (P < 0.001) was found in milk after linseed oil addition. The responses to both oil additions were relatively sustainable with regard to CLA, trans-11 18:1 (vaccenic acid, VA), and ALA content after the last 6 days of supplementation. The winter ration supplementation with sunflower or linseed oil led to a 3-fold increase in CLA milk fat content (0.6-2.0 or 1.8 g/100 g fatty acid methyl esters (FAME), P < 0.001) and a 3-fold increase in VA milk fat content (1.2-3.8 or 4.1 g/100 g FAME, P < 0.001), however the content of trans-10 18:1 was 5-6-fold higher, compared with unsupplemented winter ration. Plant oil supplementation enhanced the total content of CLA, VA, ALA by 5.0 and 3.9 g/100 g FAME (P < 0.001) for linseed and sunflower oil supplementation, and decreased the total content of 12:0, 14:0, and 16:0 by 9.3 and 5.8 g/100 g FAME (P < 0.001) compared to winter diet, respectively.

show abstract

Section: Methodsmentioning

confidence: 99%

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Soják¹,

Blaško²,

Kubinec³

et al. 2015

CZECH J ANIM SCI

View full text Add to dashboard Cite

show abstract

“…Most of the standards (Table 1) used in the method optimization were purchased from Sigma-Aldrich Chemie (Sigma-Aldrich, New South Wales, Australia), except the triarachidonin (5,8,11,14-all cis), 10t, 12c-octadecanoyl triglyceride (CLA 10t, 12c), 9c, 11t-octadecanoyl triglyceride (CLA 9c, 11t), trieicosapentaenoin (5,8,11,14,17 all cis) triglyceride and tridocosahexanoin (4,7,10,13,16,19 all cis) triglyceride standards, which were purchased from Larodan Fine Chemicals (Malmö, Sweden). Standard FAME 37 mixture and cis/trans mixture were obtained from Restek (Shimadzu -Auckland, New Zealand).…”

Section: Methodsmentioning

confidence: 99%

“…Tel. : +64 7 8385532; fax: +64 7 838 5625. technology that meets most of these requirements, however, procedures for GC analysis of milk fatty acid profiles remain time consuming [6][7][8][9][10][11], specially for large number of samples such as phenotypic investigations [5].…”

Section: Introductionmentioning

confidence: 99%

Direct analysis of fatty acid profile from milk by thermochemolysis–gas chromatography–mass spectrometry

Reis

Leath

Stelwagen

2011

Journal of Chromatography A

View full text Add to dashboard Cite

“…Since a peak overlapping was found for C 19:0 [internal standard (IS); nonadecanoate methyl ester acid; Sigma-Aldrich, St. Louis, MO, USA], the area of interfering peak was measured running each sample twice, with and without IS. In our study, the co-elution of the c9,t11+t7,c9+t8,c10 triplet (Blasko et al, 2009) was not checked owing to commercial unavailability of the t7,c9 and t8,c10 isomer standards. Nevertheless, these isomers occur in low amounts in ruminant fat (Yurawecz et al, 1998;Fritsche et al, 2000;Serra et al, 2009).…”

Section: Chemical Analysismentioning

confidence: 97%

Contents of Conjugated Linoleic Acid Isomerscis9,trans11andtrans10,cis12in Ruminant and Non-Ruminant Meats Available in the Italian Market

Cicognini

Rossi

Sigolo

et al. 2014

Italian Journal of Animal Science

View full text Add to dashboard Cite

Chemometric deconvolution of gas chromatographic unresolved conjugated linoleic acid isomers triplet in milk samples

Cited by 19 publications

References 7 publications

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Direct analysis of fatty acid profile from milk by thermochemolysis–gas chromatography–mass spectrometry

Contents of Conjugated Linoleic Acid Isomerscis9,trans11andtrans10,cis12in Ruminant and Non-Ruminant Meats Available in the Italian Market

Contact Info

Product

Resources

About