Chemometric Discrimination of the Geographical Origin of Three Greek Cultivars of Olive Oils by Stable Isotope Ratio Analysis

Abstract

Α stable isotope ratio mass spectrometer was used for stable isotope ratio (i.e., δ13C, δ18O, and δ2H) measurements, achieving geographical discrimination using orthogonal projections to latent structures discriminant analysis. A total of 100 Greek monovarietal olive oil samples from three different olive cultivars (cv. Koroneiki, cv. Lianolia Kerkyras, and cv. Maurolia), derived from Central Greece and Peloponnese, were collected during the 2019–2020 harvest year aiming to investigate the effect of botanical and geographical origin on their discrimination through isotopic data. The selection of these samples was made from traditionally olive-growing areas in which no significant research has been done so far. Samples were discriminated mainly by olive cultivar and, partially, by geographical origin, which is congruent with other authors. Based on this model, correct recognition of 93.75% in the training samples and correct prediction of 100% in the test set were achieved. The overall correct classification of the model was 91%. The predictability based on the externally validated method of discrimination was good (Q2 (cum) = 0.681) and illustrated that δ18O and δ2H were the most important isotope markers for the discrimination of olive oil samples. The authenticity of olive oil based on the examined olive varieties can be determined using this technique.

Geographical Origin Assessment of Extra Virgin Olive Oil via NMR and MS Combined with Chemometrics as Analytical Approaches

“…Interestingly, the stable isotope contents (C, O, H) correlated to the specific geoclimatic conditions of the studied Portuguese geographical area. Very recently, Tarapoulouzi et al, 2021 [ 145 ], achieved geographical discrimination among Central Greece and Peloponnese EVOOs, using IRMS measurements. A statistical model able to discriminate olive oils based on geographical origin was obtained with a successful discrimination ability at around 91%.…”

Effect of Duration of Olive Storage on Chemical and Sensory Quality of Extra Virgin Olive Oils

“…Olive oil plays an important role in the diet in Mediterranean countries [1]. Extra virgin olive oil (EVOO) is the only vegetable oil that must be extracted only by mechanical means without any adjuvants [2].…”

Authentication and Chemometric Discrimination of Six Greek PDO Table Olive Varieties through Morphological Characteristics of Their Stones

“…Martínez et al approached the problem of varietal identification by feature extraction from the analysis of endocarp images, and then using partial least square-discriminant classifier [33]. This is the first time that the research group studied the authenticity of Greek varieties of table olives, although similar research has been conducted in different countries and varieties by other authors who combined imaging and chemometrics [19,20,[30][31][32]34,35]. Esteves da Silva demonstrated the great usefulness of chemometrics in the classification of olive varieties.…”

“…Several advanced analytical techniques have been used for the study of authentication of table olives, such as high-performance liquid chromatography (HPLC) [23,28], ultra-highperformance liquid chromatography-quadrupole time of flight tandem mass spectrometry (UHPLC-QTOF-MS) [25], gas chromatography-mass spectrometry (GC-MS) [26] and nuclear magnetic resonance spectroscopy (NMR) [21]. Chemometrics is an important science which has been extensively used in food science and authenticity studies to facilitate interpretation of huge load of data, and it provides an easy way to visualize the samples [19,20,[29][30][31][32][33][34][35][36].…”