Chemometrics-assisted simultaneous determination of atenolol and chlorthalidone in synthetic binary mixtures and pharmaceutical dosage forms

Abstract

Resolution of binary mixtures of atenolol (ATE) and chlorthalidone (CTD) with minimum sample pre-treatment and without analyte separation has been successfully achieved, using a new and rapid method based on partial least squares (PLS1) analysis of UV spectral data. The simultaneous determination of both analytes was possible by PLS1 processing of sample absorbances between 255 and 300 nm for ATE and evaluation of absorbances in the 253-268 nm region for CTD. The mean recoveries for synthetic samples were 100.3 +/- 1.0% and 100.7 +/- 0.7% for ATE and CTD, respectively. Application of the proposed method to two commercial tablet preparations in the content uniformity test showed them to contain 103.5 +/- 0.8% and 104.9 +/- 1.8% ATE respectively, as well as 103.4 +/- 1.2% and 104.5 +/- 2.2% CTD. Use of this method also allowed the elaboration of dissolution profiles of the drugs in two commercial combined formulation products, through the simultaneous determination of both drugs during the dissolution test. At the dissolution time of 45 min specified by USP XXIV, both pharmaceutical formulations complied with the test.

Simultaneous Determination of Acetaminophen and Synthetic Color(s) by Derivative Spectroscopy in Syrup Formulations and Validation by HPLC: Exposure Risk of Colors to Children

“…Literature survey reveals various analytical techniques, with chromatographic methods playing a significant role in pharmaceutical analysis (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). However, these methods require sophisticated and expensive equipment, provision for use and disposal of solvents, labor-intensive sample preparation procedures, and personal skills.…”

“…anurag1706@gmail.com; mditrc@rediffmail.com) the zero-crossing techniques (21,(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40). Though absorption spectrophotometry have been reported for the estimation of individual drugs or in case of mixtures where absorption maxima are distinctly apart (41)(42)(43)(44)(45)(46)(47)(48)(49), however, no method is available for the simultaneous estimation of the drug and color additive.…”

Simultaneous determination of atenolol, chlorthalidone and amiloride in pharmaceutical preparations by capillary zone electrophoresis with ultraviolet detection

“…Hypertension therapy can be carried out using a single drug and, in those cases which are not suited to single drug therapy, the addition of a second drug is recommended. These therapies demand strict quality control measures, necessitating techniques for the determination of each of the active ingredients without interference of the others (Ferraro et al, 2003).…”

“…In some of these works, partial least squares analysis of the UV spectral data for the determination of AT and CD in synthetic binary mixtures and pharmaceutical formulations (Ferraro et al, 2003;Mohamed and Salem 2005) or for the simultaneous determination of AT, AM and CD in pharmaceutical formulations (El-Gindy et al, 2005) or for determination of AM, AT, hydrochlorothiazide and timolol maleate in synthetic mixtures and pharmaceutical formulations (Ferraro et al, 2004) was conducted.…”

Simultaneous determination of atenolol and amiloride in pharmaceutical preparations by capillary zone electrophoresis with capacitively coupled contactless conductivity detection

“…In some of these works, partial least squares analysis of the UV spectral data for the determination of AT and CD in synthetic binary mixtures and pharmaceutical formulations (Ferraro et al, 2003;Mohamed and Salem 2005) or for the simultaneous determination of AT, AM and CD in pharmaceutical formulations (El-Gindy et al, 2005) or for determination of AM, AT, hydrochlorothiazide and timolol maleate in synthetic mixtures and pharmaceutical formulations (Ferraro et al, 2004) were conducted.…”