Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative

Abstract

Abstract. the preventive effects of dietary exposure to a wasabi derivative 6-methylsulfinylhexyl isothiocyanate (6-MsItc) during the initiation and post-initiation phases on the development of 1,2-dimethylhydrazine (DMH)-induced colonic aberrant crypt foci (AcF), and β-catenin-accumulated crypts (BcAc) were investigated in male F344 rats. to induce AcF and BcAc, rats were given four weekly subcutaneous injections of DMH (40 mg/kg body weight). the rats also received diets containing 200 or 400 ppm 6-MsItc during the initiation or post-initiation phases. the experiment was terminated 12 weeks after the start. DMH exposure produced a substantial number of AcF (323.8±69.7/colon) and BcAc (3.80±1.05/cm 2 ) at the end of the study. Dietary administration of 6-MsItc at a dose of 400 ppm during the initiation phase caused a significant reduction in the total number of ACF (52% reduction, P<0.0001), larger ACF (4 or more crypt ACF) (58% reduction, P<0.001) and BCAC (76% reduction, P<0.00001). The dietary exposure to 6-MSITC significantly reduced the size (crypt multiplicity) of BcAc during both initiation and post-initiation treatment when compared to group 1 treated with DMH alone. Immunohistochemically, 6-MsItc administration lowered the proliferating cell nuclear antigen labeling index in AcF and BcAc. In addition, protein levels of hepatic cytochrome P-450 isozymes at 24 h after 6-MsItc exposure were significantly suppressed (P<0.01). The results indicated that 6-MsItc exerted chemopreventive effects in the present short-term colon carcinogenesis bioassay, through alterations in cell proliferation activity and drug metabolizing enzyme levels.

Wasabi 6‐(methylsulfinyl)hexyl isothiocyanate induces apoptosis in human colorectal cancer cells through p53‐independent mitochondrial dysfunction pathway

“…Of which, 6‐(methylsulfinyl)hexyl isothiocyanate (6‐MSITC) is a major active compound in Wasabi . Oral administration of 6‐MSITC revealed the suppressive effects on rat colon carcinogenesis induced by 1,2‐dimethylhydrazine . The results suggest that 6‐MSITC is a potential chemopreventive compound for colon carcinogenesis.…”

Wasabi Compound 6-(Methylsulfinyl) Hexyl Isothiocyanate Induces Cell Death with Coexisting Mitotic Arrest and Autophagy in Human Chronic Myelogenous Leukemia K562 Cells

“…A naturally-occurring compound, 6-(methylsulfinyl) hexyl isothiocyanate (6-MITC), was isolated from Wasabia japonica (wasabi), a pungent spice widely used in Japanese food. Several biological activities of 6-MITC have been reported, such as anti-inflammatory [17,18], neuroprotective [19,20], anti-cancer [21][22][23], and chemopreventive effects [24,25]. Our previous publication indicated that 6-MITC and its derivatives may have an inhibitory effect against pancreatic cancer cell growth (including cancer stem cells phenotype) [26].…”

Bioactive phytocompounds for oral cancer prevention and treatment: A comprehensive and critical evaluation

“…6-MITC (Figure 5), a glucosinolate isolated from Wasabia japonica (wasabi), has been linked to chemopreventive and antimetastatic effects. [194][195][196][197] In the context of oral cancer, Lee and coresearchers 103…”