Chemoprevention of a flavonoid fraction from Rhus verniciflua Stokes on aflatoxin B₁‐induced hepatic damage in mice

Abstract

Since aflatoxin B(1) (AFB(1))-mediated hepatic damage is related to the production of AFB(1)-8,9-epoxide and reactive oxygen species, bioactive compounds having antioxidant potentials are suggested to be capable of reducing AFB(1)-induced toxicity. We previously purified a mixture of flavonoids that we named RCMF (Rhus verniciflua Stokes chloroform-methanol fraction), from a traditional Korean food additive and herbal medicine. RCMF exhibited various biological effects, including antioxidant and antitumor activities. In this study, we examined whether RCMF protects against AFB(1)-induced liver injury using in vitro and in vivo systems. Pretreatment of HepG2 cells with RCMF significantly reduced AFB(1)-stimulated production of ROS and malondialdehyde (MDA) to the control levels. RCMF also prevented the reduction in HepG2 cell viability caused by AFB(1). Oral administration of RCMF to mice significantly suppressed an AFB(1)-induced increase in serum levels of alanine aminotransferase, alkaline phosphatase and lactate dehydrogenase. It also prevented MDA formation and blocked decreases in glutathione levels and superoxide dismutase activities in the livers of AFB(1)-treated mice. In addition, RCMF supplementation prevented an AFB(1) -induced decrease in serum titers of IgA and IgG1. Collectively, these results suggest that RCMF attenuates AFB(1)-mediated damage to the liver, and that this effect is at least partially related to the restoration of antioxidant defense systems and an increase in AFB(1)-GSH conjugate formation.

Effect of Dietary Curcumin on Heptorenal Alfla-Toxicosis in Mice

“…Feeding curcumin to afla-intoxicated mice resulted in significant improvement in levels of TAC and SOD. Our results were in accordance to those of Choi et al, (2011) and Abdel-Wahhab et al (2016). It was clear from the results that AFs have adverse effects on mice health via their ability to induce oxidative stress that furthermore led to the promotion of lipid peroxidation.…”

In vitro and in vivo assessment of banana peel powder as an aflatoxins biosorbent

“…Agricultural wastes such as peel of various fruits and vegetables is applied in food and other industries as an adsorption agents for various contaminants (Mohamed et al, 1994 (Kulkarni, 2005;Chanda et al, 2010;Kamba & Hassan, 2010;Mohd et al, 2012 andJanjua et al, 2013). Furthermore, several plant wastes have been shown to have ameliorative effects against induced toxicity of AFB1 in animals (Liu et al, 1995;Gyamfi & Aniya, 1998;Oluwafemi & Taiwo, 2004;Farombi et al, 2005 andChoi et al, 2011).…”

Measures against aflatoxin B1: a brief review about the use of flavonoids and clays

“…The biological activities of l avonoids cover a very broad spectrum, including anticancer, antioxidant, antiviral and antibacterial effects (SIESS et al, 2000;NONES et al, 2011;ALMEIDA et al, 2015), and several l avonoids are promising protectors when added to a CHOI et al, 2011;LANGESWARAN et al, 2012;NONES et al, 2013).…”