Chemopreventive effects of Panax notoginseng and its major constituents on SW480 human colorectal cancer cells

Abstract

Abstract. In this study, we evaluated the effects of Panax notoginseng root extract (NGRE) and its major constituents on SW480 human colorectal cancer cells. We used high performance liquid chromatography to determine the contents of major saponins in NGRE. The anti-proliferative effects were evaluated by the cell counting method, and concentrationrelated anti-proliferative effects were observed. At 1.0 mg/ml, NGRE inhibited cell growth by 85.8% (P<0.01), probably linked to the higher concentration of ginsenosides Rb1 and Rg1. The pharmacologic activities of notoginsenoside R1 and ginsenosides Rg1 and Rb1 on the cells were antiproliferative. We tested the effects of NGRE on DNA synthesis by measuring [3 H]-thymidine incorporation. NGRE induced cell apoptosis at 0.5 and 1 mg/ml. Two-day treatment with 300 μM of notoginsenoside R1, ginsenosides Rg1 and Rb1 increased cell apoptosis significantly. Cell cycle and cyclin A assay showed that NGRE arrested cells in the synthesis phase and increased the expression of cyclin A remarkably. NGRE also enhanced the actions of two chemotherapeutic agents, 5-fluorouracil and irinotecan. Cell growth decreased more with the combined treatment of NGRE and 5-fluorouracil (or irinotecan) than with the chemotherapy agent applied alone, suggesting that notoginseng can reduce the dose of 5-fluorouracil (or irinotecan) needed to achieve desired effects. Further in vivo and human trials are warranted to test whether notoginseng is a valuable chemo-adjuvant with clinical validity.

Role of Bioactive Constituents of Panax notoginseng in the Modulation of Tumorigenesis: A Potential Review for the Treatment of Cancer

“…It has also been noted that PNS have markedly cytotoxic effects on the proliferative activity of human colon cancer cell line LoVo in a time- and dose-dependent manner arresting the cell cycle at the S phase ( He et al, 2012 ). PNS have also been accountable for the inhibition of the colorectal cell line (SW480) during the S phase while significantly enhancing the expression of cyclin A and promoting apoptosis ( Wang et al, 2007 ).…”

“…Notoginsenoside R1 (NGR1), a major constituent of P. notoginseng , is regarded to curb neurotoxicity, cardiovascular diseases, osteoporosis, and cerebrovascular diseases effectively ( Jia et al, 2014 ; Yan et al, 2014 ; Wang et al, 2015 ; Yu et al, 2016 ). Earlier studies conducted have revealed the beneficiary role of NGR1 in the prevention and treatment of colon cancer and leukemia ( Xu et al, 1991 ; Wang et al, 2007 ; Wang C.-Z. et al, 2009 ).…”

Phytoadaptogens in the Tumours Biotherapy and Geriatrics (Part 2)

“…В связи с этим ФА можно рекомендовать для профилактики нефротоксичности при химиотерапии [49]. Экстракт женьшеня, а также гинзенозиды Rb1 и Rg1 усиливали цитотоксическое действие 5-фторурацила на модели культуры клеток колоректального рака человека (SW480) с повышенной индукцией апоптоза [50]. Введение схизандрина из лимонника в культуру клеток карциномы печени или рака молочной железы (MCF-7) вместе с доксорубицином повышало уровень апоптоза опухолевых Обзоры литературы клеток за счет активации каспазы 9 и подавления потенциала митохондриальных мембран.…”