Chemoreception in hydra: specific binding of glutathione to a membrane fraction

Grosvenor, William; Bellis, Susan L.; Kass-Simon, G.; Rhoads, Dennis E.

doi:10.1016/0304-4165(92)90068-6

Cited by 32 publications

(28 citation statements)

References 21 publications

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“…Binding studies show that glutamate binds to crude membrane fractions of Hydra (Bellis et al, 1991 (Grosvenor et al, 1992).…”

Section: Excitatory Transmitters and Receptors: Glutamatementioning

confidence: 99%

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Coordinated modulation of cellular signaling through ligand-gated ion channels in Hydra vulgaris (Cnidaria, Hydrozoa)

Pierobon

2012

Int. J. Dev. Biol.

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Cnidarians lack well developed organs, but they have evolved the molecular and cellular components needed to assemble a nervous system. The apparent 'simplicity' of the cnidarian nervous net does not occur at the cellular level, but rather in the organisation of conducting systems. Cnidarian neurons are in fact electrically excitable, show the typical extended morphology and are connected by chemical synapses or gap junctions. They have been regarded as peptidergic, given the wealth of neuropeptides generally distributed along neurites and in cell bodies, supporting the hypothesis of a modulatory role in neurotransmission. However, the presence of clear-cored, as well as dense-cored synaptic vesicles in cnidarian neurons suggests both fast and slow synaptic transmission mechanisms. In fact, biochemical and functional evidence indicates that classical neurotransmitters and their metabolic partners are present in cnidarian tissues, where they are involved in coordinating motility and behavior. We have identified and characterized in Hydra tissues receptors to the inhibitory and excitatory amino acid neurotransmitters, GABA, glycine and NMDA, that are similar to mammalian ionotropic receptors in terms of their biochemical and pharmacological properties. These receptors appear to regulate pacemaker activities and their physiological correlates; in the live animal, they also affect feeding behavior, namely the duration and termination of the response elicited by reduced glutathione, with opposite actions of GABA and glycine or NMDA, respectively. These results suggest that modulation of cellular signaling through ligand-gated-ion channels is an ancient characteristic in the animal kingdom, and that the pharmacological properties of these receptors have been highly conserved during evolution.

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“…Binding studies show that glutamate binds to crude membrane fractions of Hydra (Bellis et al, 1991 (Grosvenor et al, 1992).…”

Section: Excitatory Transmitters and Receptors: Glutamatementioning

confidence: 99%

“…Lenhoff (1974Lenhoff ( , 1977 described in detail the kinetics of the response to GSH, advancing the hypothesis that a specific chemoreceptor mediated this response. Later, two independent groups reported the occurrence and characterization of a GSH receptor population in Hydra tissues (Venturini, 1987;Grosvenor et al, 1992).…”

Section: The Hydra Feeding Responsementioning

confidence: 99%

Coordinated modulation of cellular signaling through ligand-gated ion channels in Hydra vulgaris (Cnidaria, Hydrozoa)

Pierobon

2012

Int. J. Dev. Biol.

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“…Almost 50 years ago, Loomis and Lenhoff suggested a role of GSH in signal transduction in Hydra (Loomis, 1955). A class of binding sites for GSH has been described (Bellis et al, 1994;Grosvenor et al, 1992), providing the basis for the behavioural response. However, up today, the GSH receptors have not been isolated.…”

Section: Gsh Functionalized Qds Target Specific Cell Types In Hydramentioning

confidence: 99%

An Ancient Model Organism to Test In Vivo Novel Functional Nanocrystals

Tortiglione¹

2011

Biomedical Engineering - From Theory to Applications

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“…However, even though many of these compounds would be expected to exert their effects through a G-protein-coupled pathway in higher eukaryotes, the presence of similar pathways in simpler metazoans cannot be taken for granted. For example in the Chlorohydra viridissima (class Hydrozoa), dopamine, serotonin, GSH and glutamate have all been identified [61][62][63] and shown to have saturable binding sites in a membrane fraction [64,65]. However, none of these putative neurotransmitters were able to affect adenylyl cyclase activity in the hydra even though the enzyme was stimulated by fluoride ions and guanine nucleotide analogues [66].…”

Section: Ancestral Metazoamentioning

confidence: 99%

The Evidence for G-Protein-Coupled Receptors and Heterotrimeric G Proteins in Protozoa and Ancestral Metazoa

New¹,

Wong²

1998

Neurosignals

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In higher eukaryotes G-protein-coupled signal transduction pathways are a common mechanism used to detect an extracellular message and transmit a signal, via a membrane-bound receptor and a heterotrimeric G protein, to second messenger producing enzymes and effector proteins. The techniques used to identify components of these pathways are increasingly being applied to protozoa and ancestral metazoa. Many of the organisms studied do seem to express functional homologues of those found in higher eukaryotes and increasingly genes encoding these proteins are being cloned. Sequence analysis of the isolated α-subunits of heterotrimeric G proteins shows that these proteins have extensive homology to their mammalian counterparts, and often show absolute sequence identity in functionally significant regions. The receptor clones isolated clearly establish that protozoa and early metazoa express proteins with seven transmembrane spanning domains. Comparisons with mammalian receptors indicate that these proteins are likely to be regulated by phosphorylation and dephosphorylation events, although the pathways which control these are yet to be identified. The postulated regulatory mechanisms and the number of homologous clones isolated from some protozoa suggest that a highly regulated system of transmembrane signalling appeared at a relatively early stage in evolution.

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Chemoreception in hydra: specific binding of glutathione to a membrane fraction

Cited by 32 publications

References 21 publications

Coordinated modulation of cellular signaling through ligand-gated ion channels in Hydra vulgaris (Cnidaria, Hydrozoa)

Coordinated modulation of cellular signaling through ligand-gated ion channels in Hydra vulgaris (Cnidaria, Hydrozoa)

An Ancient Model Organism to Test In Vivo Novel Functional Nanocrystals

The Evidence for G-Protein-Coupled Receptors and Heterotrimeric G Proteins in Protozoa and Ancestral Metazoa

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