Chemosensation and Evolution of Drosophila Host Plant Selection

Anholt, Robert R. H.

doi:10.1016/j.isci.2019.100799

Cited by 34 publications

(33 citation statements)

References 125 publications

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“…Given host adaptation in insects often accompanied by olfactory adaptation and involved OR gene selection and duplications [ 14 ], to select candidate ORs involved in SHBs’ host-finding behavior and/or pheromone communication, we investigated the ratio of non-synonymous/synonymous nucleotide substitutions ( ω = dN/dS) of OR orthologues among species, and duplicated ORs of the SHB were tested for potential positive selection events using PAML package version 4.9d [ 44 ]. This will provide information about whether these genes have undergone selection pressures, i.e., positive selection ( ω > 1) or neutral/negative selection ( ω ≤ 1) [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the chemosensory proteins (CSPs) and insect-type odorant-binding receptors (OBPs), two soluble binding protein families, are known to mediate the transport of hydrophobic ligands through the aqueous environment of the sensillar lymph and to enhance the sensitivity of the insect ORs [ 10 , 11 ]. Some evidence showed that OBPs and CSPs may participate in insect olfaction, for example, differential expression of OBPs to pheromone stimuli [ 12 ], and extremely versatile CSPs performing olfactory tasks but also others [ 13 ]; however, their precise role in modulating olfactory responses remains unclear [ 10 , 14 ]. These made them less attractive as targets for identifying key genes.…”

Section: Introductionmentioning

confidence: 99%

“…Recent discoveries pointed out that the OR gene family appears to be highly divergent across insect species [ 29 , 30 ]. Notably, the evolution of OR genes may be influenced by interactions between hosts and their parasites [ 14 ]. For example, most Drosophila species are attracted to decaying fruits, whereas Drosophila suzukii females parasitize ripening fruit, particularly, a variety of berries [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gene Expression and Functional Analyses of Odorant Receptors in Small Hive Beetles (Aethina tumida)

Liu

Beaurepaire

Rogers

et al. 2020

IJMS

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Olfaction is key to many insects. Odorant receptors (ORs) stand among the key chemosensory receptors mediating the detection of pheromones and kairomones. Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies and olfactory cues are especially important for host finding. However, how interactions with their hosts may have shaped the evolution of ORs in the SHB remains poorly understood. Here, for the first time, we analyzed the evolution of SHB ORs through phylogenetic and positive selection analyses. We then tested the expression of selected OR genes in antennae, heads, and abdomens in four groups of adult SHBs: colony odor-experienced/-naive males and females. The results show that SHBs experienced both OR gene losses and duplications, thereby providing a first understanding of the evolution of SHB ORs. Additionally, three candidate ORs potentially involved in host finding and/or chemical communication were identified. Significantly different downregulations of ORs between the abdomens of male and female SHBs exposed to colony odors may reflect that these expression patterns might also reflect other internal events, e.g., oviposition. Altogether, these results provide novel insights into the evolution of SHB ORs and provide a valuable resource for analyzing the function of key genes, e.g., for developing biological control. These results will also help in understanding the chemosensory system in SHBs and other beetles.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gene Expression and Functional Analyses of Odorant Receptors in Small Hive Beetles (Aethina tumida)

Liu

Beaurepaire

Rogers

et al. 2020

IJMS

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“…Odorant-binding proteins (OBPs), as major proteins existing in the nasal mucosa of vertebrates and the antennae of insects for the binding of odorant molecules, contribute to the recognition process of surrounding odorants in the olfactory sensation system, which usually participated in the construction of olfactory biosensors. 10 − 12 Involving as ligand selector and transporter, the OBPs could bind the odorant molecules through the specific amino acid sites in the structural cavity and transport the ligands to the odorant receptors in the cell membrane. 11 , 13 Triggering the pathway of G protein-coupled signals, the odorant receptors activate the olfactory neurons and cause the related olfactory behavioral response.…”

Section: Introductionmentioning

confidence: 99%

Detection of Bitter Taste Molecules Based on Odorant-Binding Protein-Modified Screen-Printed Electrodes

et al. 2020

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Bitter taste substances commonly represent a signal of toxicity. Fast and reliable detection of bitter molecules improves the safety of foods and beverages. Here, we report a biosensor using an easily accessible and cost-effective odorant-binding protein (OBP) of Drosophila melanogaster as a biosensitive material for the detection of bitter molecules. Based on the theoretical evaluation of the protein–ligand interaction, binding energies between the OBP and bitter molecules were calculated via molecular docking for the prediction and verification of binding affinities. Through one-step reduction, gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) were deposited on the screen-printed electrodes for improving the electrochemical properties of electrodes. After the electrodes were immobilized with OBPs via layer-by-layer self-assembly, typical bitter molecules, such as denatonium, quinine, and berberine, were investigated through electrochemical impedance spectroscopy. The bitter molecules showed significant binding properties to the OBP with linear response concentrations ranging from 10 –9 to 10 –6 mg/mL. Therefore, the OBP-based biosensor offered powerful analytic techniques for the detection of bitter molecules and showed promising applications in the field of bitter taste evaluation.

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“…Major lifestyle transitions in insects, such as the conquest of terrestrial habitats, the flight or host plant interactions, are often followed by dramatic shifts in the sensory systems (Vieira and Rozas 2011;Missbach et al 2015;D. Wang et al 2018;Almudi et al 2020;Anholt 2020).…”

Section: Introductionmentioning

confidence: 99%

Smelling in the dark: phylogenomic insights on the chemosensory system of a subterranean beetle

Balart‐García

Cieslak

Escuer

et al. 2020

Preprint

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The chemosensory system has experienced relevant changes in subterranean animals, facilitating the orientation into darkness via the perception of specific chemical signals critical to survive in this particular environment. However, the genomic basis of chemoreception in cave-dwelling fauna is largely unexplored. We generated de novo transcriptomes for antennae and body samples of the troglobitic beetle Speonomus longicornis (whose characters suggest an extreme adaptation to the deep subterranean) in order to interrogate the evolutionary origin and diversification of the chemosensory gene repertoire across coleopterans through a phylogenomic approach. Our results suggested a diminished diversity of odorant and gustatory gene repertoires compared to polyphagous epigean beetles. Moreover, S. longicornis showed a large diversity of odorant-binding proteins, suggesting an important role of these proteins in capturing airborne chemical cues. We identified a gene duplication in the ionotropic co-receptor IR25a, a highly conserved single-copy gene in protostomes involved in thermal and humidity sensing. In addition, no homologous genes to sugar receptors or the ionotropic receptor IR41a were detected. Our findings suggest that the chemosensory gene repertoire of this cave beetle may have been reshaped by the low complexity of chemical signals of this particular environment, and that gene duplication and loss may have played an important role in the evolution of genes involved in chemoreception. Altogether, our results shed light on the genomic basis of chemoreception in a cave-dwelling invertebrate and pave the road towards understanding the genomic underpinnings of adaptation to the subterranean lifestyle at a deeper level.

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Chemosensation and Evolution of Drosophila Host Plant Selection

Cited by 34 publications

References 125 publications

Gene Expression and Functional Analyses of Odorant Receptors in Small Hive Beetles (Aethina tumida)

Gene Expression and Functional Analyses of Odorant Receptors in Small Hive Beetles (Aethina tumida)

Detection of Bitter Taste Molecules Based on Odorant-Binding Protein-Modified Screen-Printed Electrodes

Smelling in the dark: phylogenomic insights on the chemosensory system of a subterranean beetle

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