Chemotactic Activity of Intestinal Strangulation Obstruction Fluid From Germfree and Conventional Rats

Abstract

The chemotactic activity of rat strangulation fluid on rat polymorphonuclear leucocytes was investigated. Chemotactic activity was demonstrated in strangulation fluid from conventional as well as from germfree rats. The chemotactic substances resisted heat treatment at 56° C for 30 min. The chemotactic activity was destroyed following heat treatment at higher temperatures. Dialysis did not remove the chemotactic activity. Gel‐filtration chromatography on Sephadex G‐100 of strangulation fluid from germfree rats resulted in fractions with three to four peaks of chemotactic activity. The possible relationship to the complement system was discussed.