Chemotactic behavior of myoblasts

Venκatasubramanian, Κ.; Solursh, Michael

doi:10.1016/0012-1606(84)90098-8

Cited by 53 publications

(25 citation statements)

References 26 publications

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“…The molecular nature of the signal provided by ECs remains to be elucidated. A crucial role for the non-myogenic cell-produced extracellular matrix for myoblasts migration has also been documented (Chiquet et al, 1981;Sanderson et al, 1986), together with a putative role of limb vessels in myoblast guidance (Venkatasubramanian and Solursh, 1984). Given the complexity of the process, a multistep control is likely and our results indicate that the EC compartment has to be integrated in this complex picture.…”

Section: Molecular Signals Involved In Limb Myoblast Emigrationsupporting

confidence: 65%

Limb bud colonization by somite-derived angioblasts is a crucial step for myoblast emigration

2012

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SUMMARYWe have combined the use of mouse genetic strains and the mouse-into-chicken chimera system to determine precisely the sequence of forelimb colonization by presomitic mesoderm (PSM)-derived myoblasts and angioblasts, and the possible role of this latter cell type in myoblast guidance. By creating a new Flk1/Pax3 double reporter mouse line, we have established the precise timetable for angioblast and myoblast delamination/migration from the somite to the limb bud. This timetable was conserved when mouse PSM was grafted into a chicken host, which further validates the experimental model. The use of Pax3 GFP/GFP knockout mice showed that establishment of vascular endothelial and smooth muscle cells (SMCs) is not compromised by the absence of Pax3. Of note, Pax3 GFP/GFP knockout mouse PSM-derived cells can contribute to aortic, but not to limb, SMCs that are derived from the somatopleure. Finally, using the Flk1 lacZ/lacZ knockout mouse, we show that, in the absence of angioblast and vascular network formation, myoblasts are prevented from migrating into the limb. Taken together, our study establishes for the first time the time schedule for endothelial and skeletal muscle cell colonization in the mouse limb bud and establishes the absolute requirement of endothelial cells for myoblast delamination and migration to the limb. It also reveals that cells delaminating from the somites display marked differentiation traits, suggesting that if a common progenitor exists, its lifespan is extremely short and restricted to the somite.

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Section: Molecular Signals Involved In Limb Myoblast Emigrationsupporting

confidence: 65%

Limb bud colonization by somite-derived angioblasts is a crucial step for myoblast emigration

2012

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“…We observed that myogenic cell migration was dependent on the presence of fibronectin, because migration was negligible when the filters were either left untreated or coated with gelatin. It has been suggested that fibronectin is required in these in vitro assays to stimulate the initial cell attachment to the polycarbonate filter (Venkatasubramanian and Solursh, 1984); however, it is also thought to be important for myogenic cell migration in vivo, because the injection of fibronectin antibodies into the limb inhibits migration of these cells (Brand-Saberi et al, 1993). The limb myogenic cells responded to both FGF-2 and FGF-4; however, compared with FGF-2, FGF-4 was less effective at stimulating myogenic cell migration.…”

Section: Discussionmentioning

confidence: 99%

“…Within the limb bud, the myogenic cells retain their invasive property and actively migrate in a proximodistal direction to establish the skeletal muscle pattern of the limb (Brand-Saberi et al, 1989, 1996aEde, 1989a,b, 1990). It has been revealed that this migration is mediated by cell-cell contacts (Lee and Ede, 1989a;Brand-Saberi et al, 1996a), cell-matrix interactions Brand-Saberi et al, 1993), and cell growth-factor interactions (Venkatasubramanian and Solursh, 1984;Bladt et al, 1995;Brand-Saberi et al, 1996b). All of these three types of interactions are crucial to the migration process in the limb.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factors 2 and 4 stimulate migration of mouse embryonic limb myogenic cells

et al. 1997

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“…Venkatasubramanian and Solursh (1984) reported that quail limb mesoderm cells respond chemotactically to plateletderived growth factor (PDGF), but the cells were tested after their migration from the somites and were not a pure population. More recently, Daston et al (1996) have shown that the transcription factor Pax-3 is necessary for emigration of myogenic cells from the somites in mouse embryos.…”

Section: Introductionmentioning

confidence: 99%