Chemotactic drift speed for bacterial motility pattern with two alternating turning events

Pankratova, Evgeniya V.; Kalyakulina, Alena; Krivonosov, Mikhail; Denisov, S. I.; Taute, Katja M.; Zaburdaev, Vasily

doi:10.1371/journal.pone.0190434

Cited by 7 publications

(6 citation statements)

References 31 publications

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“…Rl mycelium extracts and P. thunbergii root extracts can affect the chemotaxis of B. pumilus HR10, which might include its motility, attachment, growth and possibly swarming motility phases. The chemotactic properties are determined by the strength of bacterial flagellar motility ( Lapidus et al, 1988 ; Pion et al, 2013 ; Pankratova et al, 2018 ). The colonization tendency and effect of root microorganisms on the root surface are related to their swimming on soft agar surfaces and swarming inside soft agars ( Matthysse and Mcmahan, 2001 ; Esfehani et al, 2009 ; Hou et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Colonization by the Mycorrhizal Helper Bacillus pumilus HR10 Is Enhanced During the Establishment of Ectomycorrhizal Symbiosis Between Hymenochaete sp. Rl and Pinus thunbergii

et al. 2022

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There are complex interactions between mycorrhizal helper bacteria (MHBs) and ectomycorrhizal (ECM) fungi, with MHBs promoting mycorrhizal synthesis and ECM fungi regulating plant rhizobacterial colonization, diversity, and function. In this study, to investigate whether the ECM fungus Hymenochaete sp. Rl affects the survival and colonization of the MHB strain Bacillus pumilus HR10 in the rhizosphere, the biomass of B. pumilus HR10 was measured in the rhizosphere and mycorrhizosphere. In addition, extracts of Hymenochaete sp. Rl and Pinus thunbergii were evaluated for their effect on B. pumilus HR10 colonization (growth, sporulation, biofilm formation, extracellular polysaccharide and extracellular protein contents, flagellar motility, and expression of colonization-related genes). The results showed that inoculation of Hymenochaete sp. Rl significantly increased the biomass of B. pumilus HR10 in the rhizosphere; however, while extracts of Hymenochaete sp. Rl and P. thunbergii did not affect the biomass or spore formation of HR10, they did affect its biofilm formation, extracellular polysaccharide and extracellular protein production, and flagellar motility. Furthermore, the addition of symbiont extracts affected the expression of chemotaxis-related genes in HR10. When the extracts were added separately, the expression of srf genes in HR10 increased; when the extracts were added simultaneously, the expression of the flagellin gene fliG in HR10 increased, but there was no significant effect on the expression of srf genes, consistent with the results on biofilm production. Thus, Hymenochaete sp. Rl and P. thunbergii roots had a positive effect on colonization by B. pumilus HR10 at the rhizosphere level through their secretions.

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Section: Discussionmentioning

confidence: 99%

Colonization by the Mycorrhizal Helper Bacillus pumilus HR10 Is Enhanced During the Establishment of Ectomycorrhizal Symbiosis Between Hymenochaete sp. Rl and Pinus thunbergii

et al. 2022

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“…2f). The chemotactic drift velocity is expected to depend not only on swimming speed and turning frequency, but also on turning angles 38,39 , raising the question whether swimming speed differences alone account for the differences in chemotactic performance observed between the P and PL phenotypes. We thus determine chemotactic drift as a function of individual swimming speed for both populations (Fig.…”

Section: Lateral Flagella Decrease the Drift Velocity In Buffer By Lo...mentioning

confidence: 99%

Physiological adaptation in flagellar architecture improvesVibrio alginolyticuschemotaxis in complex environments

Grognot

Nam

Elson

et al. 2023

Preprint

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Bacteria navigate natural habitats with a wide range of mechanical properties, from the ocean to the digestive tract and soil, by rotating helical flagella like propellers. Species differ in the number, position, and shape of their flagella, but the adaptive value of these flagellar architectures is unclear. Many species traverse multiple types of environments, such as pathogens inside and outside a host. We investigate the hypothesis that flagellar architectures mediate environment-specific benefits in the marine pathogen Vibrio alginolyticus which exhibits physiological adaptation to the mechanical environment. In addition to its single polar flagellum, the bacterium produces lateral flagella in environments that differ mechanically from water. These are known to facilitate surface motility and attachment. We use high-throughput 3D bacterial tracking to quantify chemotactic performance of both flagellar architectures in three archetypes of mechanical environments relevant to the bacterium's native habitats: water, polymer solutions, and hydrogels. We reveal that lateral flagella impede chemotaxis in water by lowering the swimming speed but improve chemotaxis in both types of complex environments. Statistical trajectory analysis reveals two distinct underlying behavioral mechanisms: In viscous solutions of the polymer PVP K90, lateral flagella increase the swimming speed. In agar hydrogels, despite lowering the swimming speed, lateral flagella improve overall chemotactic performance by preventing trapping in pores. Our findings show that lateral flagella are multi-purpose tools with a wide range of applications beyond surfaces. They implicate flagellar architecture as a mediator of environment-specific benefits and point to a rich space of bacterial navigation behaviors in complex environments.

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“…The former assumption is inspired by the chemotactic response of several natural microswimmers (Friedrich 2008; Pankratova et al. 2018; Salek et al. 2019).…”

Section: Response Of An Unsteady Chiral Swimmer To An External Chemic...mentioning

confidence: 99%

“…For example, the ratio of perturbed to unperturbed slip coefficients is δ j, k A(1) 10 /δ j, k A(0) 10 ∼ 0.1. The former assumption is inspired by the chemotactic response of several natural microswimmers (Friedrich 2008;Pankratova et al 2018;Salek et al 2019). However, the rotation rate can be highly perturbed by the chemical gradient, i.e.…”

Section: Radial Chemical Gradientmentioning

confidence: 99%

Unsteady chiral swimmer and its response to a chemical gradient

Maity

Burada

2022

J. Fluid Mech.

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Unsteadiness occurs in the motion of swimmers while they start from rest or escape from a predator, or attack prey. In this paper, we study the behaviour of an unsteady chiral swimmer, with a prescribed surface slip velocity, in the low-Reynolds-number regime, and its response to an external chemical gradient. In the first part, by solving the unsteady Stokes equation, we calculate the migration velocity ( $\boldsymbol {U}$ ), rotation rate ( $\boldsymbol {\varOmega }$ ) and flow field of the unsteady swimmer in a closed form. We compare these results with some previously known results in appropriate limits. In the second part, we investigate the response of the unsteady chiral swimmer to an external chemical gradient, which can influence the swimmer's surface slip velocity. Consequently, the swimmer either steers towards the source of the chemical gradient or moves away from it, depending on the strengths of $\boldsymbol {U}$ and $\boldsymbol {\varOmega }$ , and the corresponding angle ( $\chi$ ) between them. Interestingly, the swimmer swims in a closed orbit in the vicinity of the chemical target, depending on the strengths of $\boldsymbol {\varOmega }$ and $\chi$ . We present a complete state diagram representing the successful, unsuccessful and orbital states for various strengths of $\boldsymbol {\varOmega }$ and $\chi$ . This study is useful to understand the unsteady propulsion of ciliated microorganisms and their response to external gradients.

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Chemotactic drift speed for bacterial motility pattern with two alternating turning events

Cited by 7 publications

References 31 publications

Colonization by the Mycorrhizal Helper Bacillus pumilus HR10 Is Enhanced During the Establishment of Ectomycorrhizal Symbiosis Between Hymenochaete sp. Rl and Pinus thunbergii

Colonization by the Mycorrhizal Helper Bacillus pumilus HR10 Is Enhanced During the Establishment of Ectomycorrhizal Symbiosis Between Hymenochaete sp. Rl and Pinus thunbergii

Physiological adaptation in flagellar architecture improvesVibrio alginolyticuschemotaxis in complex environments

Unsteady chiral swimmer and its response to a chemical gradient

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