1997
DOI: 10.1073/pnas.94.26.14495
|View full text |Cite
|
Sign up to set email alerts
|

Chemotaxis in a lymphocyte cell line transfected with C-C chemokine receptor 2B: Evidence that directed migration is mediated by βγ dimers released by activation of Gαi-coupled receptors

Abstract: Chemotaxis is mediated by activation of seven-transmembrane domain, G protein-coupled receptors, but the signal transduction pathways leading to chemotaxis are poorly understood. To identify G proteins that signal the directed migration of cells, we stably transfected a lymphocyte cell line (300-19) with G protein-coupled receptors that couple exclusively to G ␣q (the m3 muscarinic receptor), G ␣i (the -opioid receptor), and G ␣s (the ␤-adrenergic receptor), as well as the human thrombin receptor (PAR-1) and t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

8
114
0
1

Year Published

2000
2000
2015
2015

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 152 publications
(123 citation statements)
references
References 43 publications
8
114
0
1
Order By: Relevance
“…This is supported by the work of Arai et al (31), which showed that when receptors that do not normally mediate chemotaxis in the host are transfected into hemopoietic cell lines they mediate chemotaxis. Calcium mobilization alone was not sufficient to induce directed migration.…”
Section: Discussionmentioning
confidence: 74%
See 1 more Smart Citation
“…This is supported by the work of Arai et al (31), which showed that when receptors that do not normally mediate chemotaxis in the host are transfected into hemopoietic cell lines they mediate chemotaxis. Calcium mobilization alone was not sufficient to induce directed migration.…”
Section: Discussionmentioning
confidence: 74%
“…Equivalent amounts of pertussis toxin (EC 50 ϭ ϳ1-2 pg/ml) inhibited the chemotaxis of the CCR2A low and CCR2B low transfectants, whereas 2.5-to 5-fold more pertussis toxin was required to inhibit chemotaxis of the CCR2B high and CCR2B int transfectants. Arai et al (31) showed that MCP-1-stimulated chemotaxis as well as Ca 2ϩ mobilization in the mouse pre-B cell line, 300.19, are mediated through G i ␣ proteins almost exclusively, whereas the MCP-1-stimulated Ca 2ϩ flux is mediated through pertussis toxin-sensitive and -insensitive G proteins in the Jurkat, HEK, and COS-7 CCR2B transfectants (30,31). Our data would suggest that MCP-1-stimulated chemotaxis of both CCR2A and CCR2B Jurkat transfectants is almost exclusively mediated through G i ␣, whereas MCP-1-stimulated Ca 2ϩ mobilization in the Jurkat CCR2B transfectants is mediated by pertussis toxin-sensitive and -insensitive G proteins.…”
Section: Discussionmentioning
confidence: 99%
“…In contrast, coupling to the chemotaxis machinery in eosinophils is more efficient. It is unclear whether increases in intracellular calcium precede and are causally required for eosinophil chemotaxis [28,29]. However, if this is the case, then the greater efficiency of coupling to chemotaxis suggests amplification of events temporally positioned between increases in intracellular calcium and chemotaxis.…”
Section: Discussionmentioning
confidence: 99%
“…Transwell chemotaxis was assessed as described in ref. 21. Cellular actin and particulate PH-Akt-GFP were measured as described by refs.…”
Section: Methodsmentioning
confidence: 99%