Chemotaxis inCampylobacter Jejuni

Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of theCampylobacterchemoreceptor for multiple ligands (CcmL)

“…Tlp1, Tlp2, Tlp3, Tlp4, Tlp7 mc , Tlp7 m , Tlp10 and Tlp11 belong to group A, Tlp9 belongs to group B and Tlp5, Tlp6, Tlp7 c and Tlp8 belong to group C (Zautner et al, 2012). Group A Tlps have a similar structure to the methyl-accepting proteins (MCPs) found in Escherichia coli and to family A transducers of Halobacterium salinarum (Zhang et al, 1996).…”

“…C. jejuni is attracted to amino acids, organic acids and carbohydrates (Rahman et al, 2014;Hartley-Tassell et al, 2010;Hugdahl et al, 1988). The bacteria sense these chemicals via transducer-like proteins (Tlps) located in the bacterial membrane or cytoplasm (Zautner et al, 2012). Interaction between the Tlps and their respective ligands initiates a molecular signal transduction cascade that causes a change in the direction of rotation of flagellar motors.…”

“…11 putative Tlps have been identified in C. jejuni (Day et al, 2012;Zautner et al, 2012;Parkhill et al, 2000); these chemoreceptors have been classified into three groups according to their predicted domain organization. Tlp1, Tlp2, Tlp3, Tlp4, Tlp7 mc , Tlp7 m , Tlp10 and Tlp11 belong to group A, Tlp9 belongs to group B and Tlp5, Tlp6, Tlp7 c and Tlp8 belong to group C (Zautner et al, 2012).…”

“…Group A Tlps have a similar structure to the methyl-accepting proteins (MCPs) found in Escherichia coli and to family A transducers of Halobacterium salinarum (Zhang et al, 1996). They are predicted to contain an N-terminal transmembrane helix followed by a periplasmic ligand-binding domain, a second transmembrane helix and a C-terminal cytoplasmic signalling domain (Marchant et al, 2002;Zautner et al, 2012). While the signalling domain is highly conserved (and is even identical in the case of Tlp2, Tlp3 and Tlp4), the periplasmic domain exhibits high variability between different Tlps (Parkhill et al, 2000;Marchant et al, 2002).…”

Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of theCampylobacterchemoreceptor for aspartate A (CcaA)

“…In chemotaxis, swimming of motile bacteria may be controlled by various extracellular chemical gradients. These external stimuli are detected by the sensory domains of methylaccepting chemotactic proteins (MCPs) or transducer-like proteins (Tlps), which transfer information through their signalling domains, activating signalling cascades that control the direction of rotation of flagella (Zhulin, 2001;Fernando et al, 2007;Zautner et al, 2012).…”