Chemotaxis towards, adhesion to, and growth in carp gut mucus of two <i>Aeromonas hydrophila</i> strains with different pathogenicity for common carp, <i>Cyprinus carpio</i> L.

Marel, Marian van der; Schroers, Verena; Neuhaus, Henner; Steinhagen, Dieter

doi:10.1111/j.1365-2761.2008.00902.x

Cited by 57 publications

(40 citation statements)

References 47 publications

(67 reference statements)

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“…After adhesion of bacteria to mucus, further steps are necessary to cause an infection. A. hydrophila 38 grew better than A. hydrophila 60 in mucus-supplemented media (Van der Marel et al 2008). Also in the present study, the cytotoxicity of A. hydrophila 38 was higher than those of the 2 other A. hydrophila strains.…”

Section: Discussionsupporting

confidence: 51%

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Influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion

Schroers

Marel²,

Steinhagen³

2008

Dis. Aquat. Org.

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The first step of the pathogenesis of many infectious diseases is the colonisation of the mucosal surface by the pathogen. Bacterial colonisation of the mucosal surface is promoted by adherence to high molecular weight mucus glycoproteins. We examined the effect of carp intestinal mucus glycoproteins on the adhesion of different bacteria. The bacteria used were 3 strains of Aeromonas hydrophila, and A. salmonicida, Edwardsiella tarda and Yersinia ruckeri. All bacteria adhered to mucus, but at varying intensities. All tested bacteria adhered best to molecules of 670 to 2000 kDa in size, less to molecules larger than 2000 kDa and weakest to molecules of 30 to 670 kDa. In general, bacteria that showed a stronger adhesion to intestinal mucus were cytotoxic to cells in vitro, and bacteria that showed a weaker adhesion to intestinal mucus did not lead to alterations of monolayers of EPC-cells. Furthermore, the involvement of glycan side chains of the glycoproteins for bacterial adhesion was analysed for one A. hydrophila strain. After cleavage of terminal sugar residues by treatment of mucus glycoproteins with different glycosidases, binding of bacteria was modulated. When mannose was cleaved off, adhesion significantly increased. Blocking of glycan receptors by incubation of bacteria with different oligosaccharides had no clear effect on bacterial binding to mucus glycoproteins. Our results suggest that bacteria interact with carbohydrate side chains of mucus glycoproteins, and that the carbohydrates of the core region are involved in bacterial binding.

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Section: Discussionsupporting

confidence: 51%

“…Binding of mucus to the plate was confirmed by a lectin binding assay using ConA as described previously (Neuhaus et al 2007b). Bacterial adhesion to mucus was assessed as described previously (Van der Marel et al 2008). In brief, fluorescently labelled bacteria (25 µl of 10 9 CFU ml -1 ) were added in triplicate to plates coated with mucus.…”

Section: Animalsmentioning

confidence: 99%

Influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion

Schroers

Marel²,

Steinhagen³

2008

Dis. Aquat. Org.

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“…Furthermore, mucins bind a range of bacteria and constitute an important part of the mucosal defense against infection (20). However, it has also been suggested that binding to mucins could provide the pathogen with an adhesion site and thereby instead promote infection (22), although no conclusive evidence for this exists. The mucous niche is very unstable, and pathogen binding to mucins may instead serve as a decoy for the more intimate adherence that can occur between the pathogen and, e.g., glycolipids of the cell membrane.…”

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confidence: 98%

Aeromonas salmonicida Binds Differentially to Mucins Isolated from Skin and Intestinal Regions of Atlantic Salmon in an N -Acetylneuraminic Acid-Dependent Manner

et al. 2014

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b Aeromonas salmonicida subsp. salmonicida infection, also known as furunculosis disease, is associated with high morbidity and mortality in salmonid aquaculture. The first line of defense the pathogen encounters is the mucus layer, which is predominantly comprised of secreted mucins. Here we isolated and characterized mucins from the skin and intestinal tract of healthy Atlantic salmon and studied how A. salmonicida bound to them. The mucins from the skin, pyloric ceca, and proximal and distal intestine mainly consisted of mucins soluble in chaotropic agents. The mucin density and mucin glycan chain length from the skin were lower than were seen with mucin from the intestinal tract. A. salmonicida bound to the mucins isolated from the intestinal tract to a greater extent than to the skin mucins. The mucins from the intestinal regions had higher levels of sialylation than the skin mucins. Desialylating intestinal mucins decreased A. salmonicida binding, whereas desialylation of skin mucins resulted in complete loss of binding. In line with this, A. salmonicida also bound better to mammalian mucins with high levels of sialylation, and N-acetylneuraminic acid appeared to be the sialic acid whose presence was imperative for binding. Thus, sialylated structures are important for A. salmonicida binding, suggesting a pivotal role for sialylation in mucosal defense. The marked differences in sialylation as well as A. salmonicida binding between the skin and intestinal tract suggest interorgan differences in the host-pathogen interaction and in the mucin defense against A. salmonicida.

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“…However, the fish often develop hemorrhagic septicemia caused by the Gram-negative bacterium, Aeromonas hydrophila, which is a part of the environment flora and becomes infectious during stress (van der Marel et al 2008). Therefore, expression of Hsp genes during A. hydrophila served a dual response to infection and stress.…”

Section: Introductionmentioning

confidence: 99%

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Das

Mohapatra

Sahoo

2015

Cell Stress and Chaperones

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Chemotaxis towards, adhesion to, and growth in carp gut mucus of two Aeromonas hydrophila strains with different pathogenicity for common carp, Cyprinus carpio L.

Cited by 57 publications

References 47 publications

Influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion

Influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion

Aeromonas salmonicida Binds Differentially to Mucins Isolated from Skin and Intestinal Regions of Atlantic Salmon in an N -Acetylneuraminic Acid-Dependent Manner

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

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