Chemotherapeutic Effects of Bioassay-Guided Extracts of the American Cockroach,<i>Periplaneta americana</i>

Wang, Xiaoyu; He, Zheng Chun; Song, Liyan; Spencer, Shawn D.; Yang, Lei; Peng, Fang; Liu, Guangming; Hu, Minghui; Li, Hai Bo; Wu, Xiumei; Zeng, Shaohua; Hilgenfeld, Rolf; Stöckigt, Joachim; Zhao, Yu; Qian, Jin Fu

doi:10.1177/1534735411413467

Cited by 25 publications

(25 citation statements)

References 63 publications

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“…The therapeutic role of PAE in wound healing has been demonstrated clinically [19–21]. However, due to the complex chemical composition, its potential molecular mechanisms are still unclear.…”

Section: Discussionmentioning

confidence: 99%

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal‐Regulated Kinase Signaling

Qin

Xie

Zhang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Periplaneta americana extracts (PAEs) exhibit wound healing properties. However, the underlying molecular mechanisms are not well understood. Here, we treated human skin fibroblasts (HSF) with PAE and the proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The wound healing and transwell migration assays were used to detect cell migration. Nuclear factor kappa B (NF-κB) and extracellular signal-regulated kinase (ERK) pathways were analyzed by Western blot (WB). Immunofluorescence staining was used to detect the key molecular localization in the cells. The results showed that PAE enhanced the proliferation and migration of HSF cells. The expression and activation of key proteins such as RelA and p-ERK were increased in NF-κB and ERK pathways followed by nuclear translocation. In vivo, both WB and immunohistochemical (IHC) staining showed that PAE enhanced p-IκBα and p-ERK activation and the nuclear translocation of RelA. Our study suggests that the protective function of PAE is mediated via enhanced NF-κB and ERK signaling.

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Section: Discussionmentioning

confidence: 99%

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal‐Regulated Kinase Signaling

Qin

Xie

Zhang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…In some cases, LC/HRMS and NMR dereplication allow identify the bioactive components at this stage, but in most cases, regrowth on a large scale (1-3 L) is needed to have enough quantity of the bioactive compounds, whose structure elucidation is eventually performed using LC-HRMS and nuclear magnetic resonance (NMR) [21]. In the case of NPs from plants or invertebrates, similar bioassay-guided fractionation, dereplication, elucidation and chromatographic purification steps are followed [22,23].…”

Section: Natural Products In Drug Discoverymentioning

confidence: 99%

Drug Discovery from Natural Products for Pancreatic Cancer

Ramos¹,

Genilloud²,

Reyes³

et al. 2018

Advances in Pancreatic Cancer

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Since ancient times, natural products (NPs) have been used as anti-infectives, anti-inflammatories, antioxidants, analgesics and antitumorals and many compounds derived from NPs are in clinical use. The use of plants in traditional medicine for multiple purposes is well known, and throughout recent history, metabolites of microbial origin have had an extraordinary impact on the welfare of humanity. There is an outstanding diversity of chemical structures that nature, and especially microorganisms, are able to produce, due to millenniums of evolution. Since only a small amount of the world's biodiversity has been evaluated for potential biological activity, many more useful natural lead compounds await discovery, the challenge being how to access this natural chemical diversity. However, the validation and selection of primary screening assays, both phenotypic and target-based, are vital to guaranteeing a selection of extracts or molecules with relevant pharmacological action. The screening of antitumor agents against pancreatic cancer (PC) involves the use of established cell lines, cancer stem cells and spheroids that mimic the patient's tumor. Improvements in the discovery of natural products along with the emergence of new technologies in cancer screening assays, promise the discovery of new and valuable drugs to tackle pancreatic cancer in the coming years.

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“…Complementary and alternative medicine (CAM) is increasingly accepted in United States both as treatment for illness and as self-care to promote health and well-being (3). The use of CAM has increased substantially in conventional and western medical practices over the past decade, notably in cancer treatment (4). CAM includes traditional Chinese medicine (TCM) and acupuncture.…”

Section: Introductionmentioning

confidence: 99%

Kangfuxin promotes apoptosis of gastric cancer cells through activating ER-stress and autophagy

Chen

Ma²,

Lin

et al. 2017

Molecular Medicine Reports

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Gastric cancer is a leading cause of cancer‑associated mortality worldwide. In studies on the mechanisms of antigastric cancer drugs, autophagy and endoplasmic reticulum (ER) stress have been demonstrated to serve an active role in gastric cancer. The organic extract of Periplaneta americana (also termed American Cockroach), which is named Kangfuxin (KFX) in China, has been used clinically as a traditional Chinese medicine against disorders, including stomach bleeding, gastric ulcers, tuberculosis, burns and trauma. However, the role of KFX and its mechanism in gastric cancer remains to be elucidated. The present study aimed to determine the effects of KFX in vitro against cultured the human carcinoma SGC‑7901 cell line, and to explore the potential mechanism of the anticancer effects of KFX in gastric cancer. SGC‑7901 cells were treated with different concentrations of KFX for varying amounts of time. As a result, KFX treatment decreased the ratio of apoptosis regulators Bcl‑2/Bax, activated ER stress and induced significant apoptosis in SGC‑7901 cells. Furthermore, KFX was able to restore the ER stress activation blocked by 4‑phenylbutyrate. In addition, KFX activated autophagy in SGC‑7901 cells. These results demonstrated that ER stress, autophagy and the apoptosis‑inducing effects of KFX in SGC‑7901 cells may achieve promising anticancer effects in numerous other types of cancer. In particular, ER stress may serve an essential role in KFX‑induced anticancer effects on gastric carcinoma and a secondary role in autophagy.

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Chemotherapeutic Effects of Bioassay-Guided Extracts of the American Cockroach,Periplaneta americana

Cited by 25 publications

References 63 publications

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal‐Regulated Kinase Signaling

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal‐Regulated Kinase Signaling

Drug Discovery from Natural Products for Pancreatic Cancer

Kangfuxin promotes apoptosis of gastric cancer cells through activating ER-stress and autophagy

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