Chemotherapy for Advanced Pancreatic Cancer

Okines, Alicia; Ratnayake, Gihan; Chau, Ian; Cunningham, David

doi:10.1007/978-0-387-77498-5_37

Cited by 1 publication

(1 citation statement)

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“…Poor prognosis and few effective therapies make it the 8 th major form of cancer-related death, resulting in more than 227,000 deaths annually worldwide (Biankin et al 2012;Ghaneh et al 2010;Jiao and Li 2010). Concerted attempts over the past 15 years to improve the survival rate of patients diagnosed with pancreatic cancer have, in large parts been unsuccessful, and have lagged well behind advancements in the treatment of other cancers (Okines et al 2010;Vaccaro et al 2012).…”

Section: Introductionmentioning

confidence: 99%

In vitro anticancer properties of selected Eucalyptus species

Bhuyan

Sakoff

Bond

et al. 2017

In Vitro Cell.Dev.Biol.-Animal

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In spite of the recent advancements in oncology, the overall survival rate for pancreatic cancer has not improved over the last five decades. Eucalypts have been linked with cytotoxic and anticancer properties in various studies; however, there is very little scientific evidence that supports the direct role of eucalypts in the treatment of pancreatic cancer. This study assessed the anticancer properties of aqueous and ethanolic extracts of four Eucalyptus species using an MTT assay. The most promising extracts were further evaluated using a CCK-8 assay. Apoptotic studies were performed using a caspase 3/7 assay in MIA PaCa-2 cells. The aqueous extract of Eucalyptus microcorys leaf and the ethanolic extract of Eucalyptus microcorys fruit inhibited the growth of glioblastoma, neuroblastoma, lung and pancreatic cancer cells by more than 80% at 100 μg/mL. The E. microcorys and Eucalyptus saligna extracts showed lower GI values than the ethanolic Eucalyptus robusta extract in MIA PaCa-2 cells. Aqueous E. microcorys leaf and fruit extracts at 100 μg/mL exerted significantly higher cell growth inhibition in MIA PaCa-2 cells than other extracts (p < 0.05). Statistically similar IC values (p > 0.05) were observed in aqueous E. microcorys leaf (86.05 ± 4.75 μg/mL) and fruit (64.66 ± 15.97 μg/mL) and ethanolic E. microcorys leaf (79.30 ± 29.45 μg/mL) extracts in MIA PaCa-2 cells using the CCK-8 assay. Caspase 3/7-mediated apoptosis and morphological changes of cells were also witnessed in MIA PaCa-2 cells after 24 h of treatment with the extracts. This study highlighted the significance of E. microcorys as an important source of phytochemicals with efficacy against pancreatic cancer cells. Further studies are warranted to purify and structurally identify individual compounds and elucidate their mechanisms of action for the development of more potent and specific chemotherapeutic agents for pancreatic cancer.

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Section: Introductionmentioning

confidence: 99%