Chemotherapy of apple shoots in vitro as method of viruses eradication

Ромаданова, Н.В.; Толеген, А.Б.; Köken, Tülay; Nurmanov, M.; Кушнаренко, С. В.

doi:10.26577/ijbch.2021.v14.i1.04

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…Only recently, they have sought to propagate disease-resistant rootstocks. The main diseases spread in apple gardens in Kazakhstan are fire blight [5,43,45], apple scab [46,47], powdery mildew [48,49], and diseases of viral origin [50][51][52].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Fire Blight Resistance of Eleven Apple Rootstocks Grown in Kazakhstani Fields

Kairova,

Pozharskiy,

Daulet

et al. 2023

Applied Sciences

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Clonal rootstocks of apple trees are one of the main components of intensive horticulture, and they play an important role in regulating tree growth, yield, fruit quality, and resistance to biotic and abiotic stresses. In the two-year monitoring survey reported here, eleven rootstocks mainly used for cultivar grafting in Kazakhstan were assessed for fire blight resistance, taking genetic background into consideration. The loci FB_F7 on LG7 and FB_MR5 and RLP1 on LG3 were analyzed on the basis of SCAR and SNP markers. The rootstocks ‘Geneva 41’, ‘62-396’, and ‘Geneva16’, bearing resistant alleles for five markers (AH0JFXM, AH21B92, AH4AAGA, AH5I8MI, and AH6R6SQ), four markers (AH0JFXM, AH21B92, AH4AAGA, and AH5I8MI), and one (AH4AAGA) SNP marker, respectively, were not infected during the monitoring period despite weather conditions in 2022 being favorable for the development and spread of Erwinia amylovora. No connection was identified between the presence of a resistant allele for the AE10-375 marker and fire blight resistance responses. This is the first study to evaluate the fire blight resistance of foreign and local rootstocks grown in the Kazakhstan southern region.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Fire Blight Resistance of Eleven Apple Rootstocks Grown in Kazakhstani Fields

Kairova,

Pozharskiy,

Daulet

et al. 2023

Applied Sciences

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“…Micropropagation is one of the advanced methods for vegetative propagation of plants [ 1 , 2 , 3 ]. In vitro tissue culture supplements conventional propagation methods in the production of healthy, disease-free plants and allows the propagation of a large number of progeny plants with desirable traits throughout the year in a short time and reduced space [ 3 , 4 , 5 , 6 , 7 , 8 ]. In addition, micropropagation plays a key role in the conservation of plant genetic resources [ 1 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Plant Preservative MixtureTM on Endophytic Bacteria Eradication from In Vitro-Grown Apple Shoots

Ромаданова

Толеген

Кушнаренко

et al. 2022

Plants

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Endophytic contaminants are a common problem for the in vitro propagation of woody plants and have significant economic repercussions for the conservation of plant genetic resources and commercial micropropagation. In this study, first, the microbial contamination that appeared around the base of in vitro-grown apple shoots was identified as Bacillus megaterium. Then, plant preservative mixture (PPMTM) was used as a bactericidal agent in plant tissue culture. Its efficacy for eradicating endophytic B. megaterium in in vitro cultures of apple was tested. In vitro-contaminated shoots were grown in tissue culture medium supplemented with 0.2% v/v PPMTM for 12 weeks and then transferred to medium without any PPMTM and cultured for 24 weeks. This study showed that PPMTM is an effective agent for controlling the growth of B. megaterium. Our results highlight the species-specific response of apple shoots to PPMTM. PPMTM was effective in controlling endogenous microbial contaminations from apple varieties ‘Golden Delicious’, ‘Landsberger Renette’, ‘Suislepper’, and ‘Aport krovavo-krasnyi’; meanwhile, in ‘KG 7’ and ‘Gold Rush’, all the plants grown in the absence of PPMTM were still bacterially contaminated, even though they were pre-treated for 12 weeks in PPMTM-supplemented medium. These results therefore suggest the essentiality of further testing of extended incubation of PPMTM in these cultivars that had outbreaks of bacterial contamination.

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“…Кроме того, в ИББР проводится работа по оздоровлению in vitro коллекции от вирусов методами химио-, крио-и термотерапии. На данный момент создана безвирусная in vitro коллекция яблони и картофеля, работа продолжается (Romadanova et al, 2016a;Kushnarenko et al, 2017;Romadanova et al, 2021b). Коллекция in vitro используется для научных целей, для создания криогенного банка и для получения саженцев.…”

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Conservation of plant biodiversity by biotechnology methods

Ромаданова

Кушнаренко

2023

Trudy po prikladnoy botanike, genetike i selektsii

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Several methods of plant preservation are currently used in the Republic of Kazakhstan: 1) field gene banks in natural growing areas and pomological collections; 2) preservation of seeds at +4°С, –18°С, and –196°С; 3) сold storage of in vitro shoots at +4°С and +10 ± 2°С; 4) cryopreservation of plant tissues and organs at –196°C; 5) preservation of plant DNA at –80°C.Ex situ field collections of plants in Kazakhstan are maintained in botanical gardens of the Republic, arboretums, pomological gardens, nurseries, peasant and farm enterprises, and personal plots. The largest collection of fruit and berry plants is located in the Pomological Garden and includes about 4 thousand varieties, more than 40 thousand hybrids and wild forms of various crops.The Republic of Kazakhstan has about 42,000 accessions in the seed collection. The cereal crop collection is mainly concentrated in the Kazakh Research Institute of Agriculture and Plant Growing – 1/3 of the national genetic resources. The main seed collection of vegetable crops and potatoes (14,700 accessions) is held by the Kazakh Research Institute of Potato and Vegetable Growing. Collections are also available at the Institute of Botany and Phytointroduction and in the Institute of Plant Biology and Biotechnology (IPBB), with the storage mode of + 4°C and –18°C. IPBB also uses cryopreservation technology at –196°C for seed storage.The main in vitro collection of Kazakhstan is kept at the IPBB at + 4°С and +10 ± 2°С; it includes more than 140 accessions of various fruit, berry, nut, vegetable, ornamental, and woody crops, grapes, potatoes, etc. A cryogenic collection of seeds, shoot tips, dormant buds, and embryonic axes in liquid nitrogen (–196°C) is found only in IPBB. The collection includes more than 700 accessions.

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Chemotherapy of apple shoots in vitro as method of viruses eradication

Cited by 5 publications

References 4 publications

Evaluation of Fire Blight Resistance of Eleven Apple Rootstocks Grown in Kazakhstani Fields

Evaluation of Fire Blight Resistance of Eleven Apple Rootstocks Grown in Kazakhstani Fields

Effect of Plant Preservative MixtureTM on Endophytic Bacteria Eradication from In Vitro-Grown Apple Shoots

Conservation of plant biodiversity by biotechnology methods

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