Chemotypes of<i>Vibrio parahaemolyticus</i>lipopolysaccharides

Miyano, Keiichi; Ishibashi, Masanori; Kunita, Nobuharu; Takeda, Yoshifumi; Miwatani, Toshio

doi:10.1111/j.1574-6968.1980.tb05023.x

Cited by 11 publications

(4 citation statements)

References 25 publications

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“…Three unidentified amino sugars, Yl, Y2 and Y3 were isolated from V. parahaemolyticus RIMD 2210482 (O12:K19), V. parahaemolyticus RIMD 2210010 (08 : K21) and V. parahaemolyticus RIMD 2210003 (OI:K1), respectively [1]. All of them (Y1-Y3) gave positive reactions for 6-deoxyhexose.…”

Section: Resultsmentioning

confidence: 99%

“…From analyses Of amino sugars, neutral sugars and 2-keto-3-deoxyoctonate by gas-liquid chromatography the lipopolysaccharides of Vibrio parahaemolyticus were classified into 8 chemotypes [1]. During the analyses, we obtained 3 unidentified amino substances and 4 unidentified neutral substances.…”

Section: Preparation Of Lipopolysaccharidesmentioning

confidence: 99%

“…The numbers and serotypes of these strains were as follows: V. parahaemolyticus RIMD 2210003 (OI:K1), V. parahaemolyticus RIMD 2210010 (O8:K21) and V. parahaemolyticus RIMD 2210482 (O12:K19). Lipopolysaccharides were prepared as described previously [1]. Bacteria were cultured on nutrient agar containing 3% NaC1 at 37°C for 20 h. Then the cells were collected and washed with 3% NaC1 solution, and lipopolysaccharide was extracted as described by Torii et al [2].…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Gas-liquid chromatography of amino sugars was carried out as described previously [1]. The amino sugars were N-acetylated with sodium bicarbonate and acetic anhydride and then converted to alditol acetates as described by Sawardeker et al [7].…”

Section: Gas-hquid Chromatography Of Amino Sugarsmentioning

confidence: 99%

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Identification of amino sugars of Vibrio parahaemolyticus lipopolysaccharides

Miyano¹

1982

FEMS Microbiology Letters

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Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Lipopolysaccharidesmentioning

confidence: 99%

Section: Bacterial Strainsmentioning

confidence: 99%

Section: Gas-hquid Chromatography Of Amino Sugarsmentioning

confidence: 99%

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Identification of amino sugars of Vibrio parahaemolyticus lipopolysaccharides

Miyano¹

1982

FEMS Microbiology Letters

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Sugar Composition of Lipopolysaccharides of Family Vibrionaceae—Absence of 2-Keto-3-Deoxyoctonate (KDO) with the Exception of Vibrio Parahaemolyticus 06 and Plesiomonas Shigelloides—

Hisatsune

Kondo

Iguchi

et al. 1983

Advances in Research on Cholera and Related Diarrheas

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A comparative study was carried out on the sugar composition of lipo-polysaccharides (LPS) isolated from representative strains of members of the family Vibrionaceae including all of the constituting genera, i.e., Vibrio, Aeromonas, Photobacterium, Plesiomonas, and Lucibacterium. More than 100 strains were examined. It was found that, with the exception of Vibrio parahaemolyticus 06, 2-keto-3-deoxyoctonate (KDO), known generally as a component sugar in the core region of usual gram-negative bacterial LPS, is virtually absent from LPS of the Vibrio-naceae strains so far examined. Furthermore, mannose was also lacking in LPS of Vibrionaceae strains with the exception of only one strain, A. anaerogenes (ATCC 15467). Instead, some KDO-like substances were found in LPS from Vibrio ("Beneckea H) nereida (ATCC 25917) and Plesiomonas shigelloides including the type strain (ATCC 14029), the same as those found in LPS from V. parahaemolyticus 07 and 012, and three strains of V. alginolyticus. These substances were strongly positive in the periodate-thiobarbituric acid test, yielding a color with maximum absorption at 549 nm. The spectra were identical to that of KDO, whereas substances differed from KDO at least in behavior in high-voltage paper electro-phoresis and thin-layer chromatography. A particularly interesting feature from the chemotaxonomical point of view was found in the sugar composition of LPS isolated from V. cholerae. Fructose was present exclusively in LPS of V. cholerae (both 01 and non-Ol groups and classical and eltor biotypes) with the exception of one strain of Photobacterium phosphoreum (NCMB 844). In addition, a pair of rarely occurring amino sugars, perosamine and quinovosamine, was found in LPS from 0 I group V. cholerae regardless of either the biotype (classical or eltor) or the serotype (Inaba or Ogawa), whereas this pair was not present in non-Ol group V. cholerae (the so-called NAG vibrios). This feature was confirmed with LPS from more than 30 additional strains of 0 I group V. cholerae isolated from patients. The virtual absence of KDO in LPS of the family Vibrionaceae was demonstrated for the first time in this study. These results are compatible with the in-649

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