Chemotyping of terrestrial Nostoc-like isolates from alkali grassland areas by non-targeted peptide analysis

Riba, Milán; Kiss‐Szikszai, Attila; Gonda, Sándor; Parizsa, Péter; Deák, Balázs; Török, Péter; Valkó, Orsolya; Felföldi, Tamás; Vasas, Gábor

doi:10.1016/j.algal.2020.101798

Cited by 10 publications

(15 citation statements)

References 108 publications

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“…Therefore, the analysis confirms the end of bloom episode and the quality of the water already allowed its use without any risk associated with algae. The present results agree with recent studies in which analysis of cyanotoxins and CPs in surface waters reveals more than MC [31,32,35,41,42,51,65,67,68]. However, these cyanopeptides have received little attention and there are few toxicity and health risk studies about APs and OCA Y.…”

Section: Case Study Descriptionsupporting

confidence: 91%

“…In the samples received in September from the Susqueda and El Pasteral reservoirs, the presence of APs at the trace level in the intracellular fraction was earlier detected. Additionally, in many other previous studies, these peptides have already been found in cyanobacterial blooms and it is typical to find them associated with MCs [31,35,41,42,51,65,67,68]. These peptides are bioactive and are commonly produced by species such as Anabaena and Planktothrix [11,15,16].…”

Section: Suspect Screening By Lc-hrmsmentioning

confidence: 72%

“…As expected, due to the high content of algae presented, the superficial scums sample is the one with the highest concentration of bioactive metabolites at the intracellular level. Although recently these peptides are detected frequently worldwide [31,35,41,42,51,65,67,68], to our knowledge, no previous studies have detected these bioactive peptides at such high levels. In general, the main CPs detected for the samples analyzed were: AP F > AP B > OCA Y.…”

Section: Suspect Screening By Lc-hrmsmentioning

confidence: 82%

“…Tandem mass spectrometry (MS/MS) has been widely adopted for the routine quantitative analyses of target cyanotoxins in water samples using the very sensitive multiple reaction monitoring (MRM) mode of triple quadrupole (QqQ) mass spectrometers [23,[25][26][27][28][29][30][31]. For structural characterization and quantitation purposes, 3D ion trap (IT) [32][33][34][35][36][37], linear ion trap (LIT) [38], time-of-flight (TOF) [39,40], Q-TOF [41][42][43][44][45] and Orbitrap mass spectrometers [26,[46][47][48][49][50][51][52] were employed. High mass accuracy of TOF and Orbitrap instruments is beneficial for LC-MS and LC-MS/MS analyses of cyanotoxins [24].…”

Section: Introductionmentioning

confidence: 99%

“…To our knowledge, such high concentrations of cyanobacterial peptides (CPs) have never been reported before. Finally, it is important to highlight that effective consumer protection requires efficient detection of the whole spectrum of cyanobacterial toxin congeners, especially, considering that different bioactive metabolites are often detected simultaneously [31,32,35,38,41,42,51]. The HRMS approach has been demonstrated to be particularly suitable for the detection and structural confirmation of other CPs, more than MCs, which are not commonly monitored.…”

Section: Introductionmentioning

confidence: 99%

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High Levels of Anabaenopeptins Detected in a Cyanobacteria Bloom from N.E. Spanish Sau-Susqueda-El Pasteral Reservoirs System by LC–HRMS

Flores

Caixach

2020

Toxins

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The appearance of a bloom of cyanobacteria in the Sau-Susqueda-El Pasteral system (River Ter, NE Spain) in the autumn of 2015 has been the most recent episode of extensive bloom detected in Catalonia. This system is devoted mainly to urban supply, regulation of the river, irrigation and production of hydroelectric energy. In fact, it is one of the main supply systems for the metropolitan area of cities such as Barcelona and Girona. An assessment and management plan was implemented in order to minimize the risk associated to cyanobacteria. The reservoir was confined and periodic sampling was carried out. Low and high toxicity was detected by cell bioassays with human cell lines. Additionally, analysis studies were performed by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography–high resolution mass spectrometry (LC–HRMS). A microcystin target analysis and suspect screening of microcystins, nodularins, cylindrosperpmopsin and related cyanobacterial peptides by LC–HRMS were applied. The results for the analysis of microcystins were negative (<0.3 μg/L) in all the surface samples. Only traces of microcystin-LR, -RR and -dmRR were detected by LC–HRMS in a few ng/L from both fractions, aqueous and sestonic. In contrast, different anabaenopeptins and oscillamide Y at unusually high concentrations (µg-mg/L) were observed. To our knowledge, no previous studies have detected these bioactive peptides at such high levels. The reliable identification of these cyanobacterial peptides was achieved by HRMS. Although recently these peptides are detected frequently worldwide, these bioactive compounds have received little attention. Therefore, more studies on these substances are recommended, especially on their toxicity, health risk and presence in water resources.

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