Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. II. Characterization of a new species in the genus Partitivirus

Abstract

Short CommunicationCherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. II.

Species of Heterobasidion host a diverse pool of partitiviruses with global distribution and interspecies transmission

“…Three of the virus strains determined in this study grouped together with the ACD-associated mycovirus and the Cherry chlorotic rusty spot-associated mycovirus (Coutts et al 2004), as well as with several alphacryptoviruses of plants (Beet cryptic virus 1, Carrot cryptic virus, White clover cryptic virus 1 and Vicia cryptic virus; Fig 2). HetRV4-pa1 from the Finnish H. parviporum isolate RT3.49C (Table 2) resembled the previously described H. parviporum partitivirus Fr110B (Ihrmark 2001) with 98.6 % nucleotide similarity (Fig 2).…”

“…A nucleotide sequence alignment was generated using all the RdRp sequences determined during the present study, the HaV, Heterobasidion parviporum partitivirus Fr110B, as well as two representatives of closely related virus strains from hosts other than Heterobasidion: the Amasya Cherry Disease (ACD)-associated mycovirus (Coutts et al 2004) and the Beet cryptic virus 1 (Szego et al 2010). The nucleotide sequences were translated after the removal of 5 0 and 3 0 untranslated regions (UTR's) and aligned manually based on initial MAFFT and ClustalW alignments.…”

The effects of dsRNA mycoviruses on growth and murine virulence of Aspergillus fumigatus

“…CVRP-F (5 0 -ATGACATTCGGACATA CCAAC-3 0 ) and CVRP-R (5 0 -TTAGCCATACTGCAAATTACT-3 0 ) and CVCP-F (5 0 -ATGGCTATGGCAACATACAA-3 0 ) and CVCP-R (5 0 -ACTT ACTCCTCTCTGTTGAAC-3 0 ) respectively (Jamal et al, 2010); and the RdRP gene predicted from the sequence of AfuPV-1 dsRNA 1 (PVR-F (5 0 -ATGGAAGATTATACTCAAGATC-3 0 ) and PVR-R (5 0 -GC CATAGGCGTAGAAGATTGAT-3 0 ) (Bhatti et al, in press). RT-PCR amplification was performed as described previously and the products examined on agarose gels stained with ethidium bromide (Coutts et al, 2004).…”

A novel mycovirus from Aspergillus fumigatus contains four unique dsRNAs as its genome and is infectious as dsRNA

“…After electrophoretic separation on agarose gels, dsRNAs 1-4 were used, either collectively or individually, for reverse transcription, PCR amplification, cloning, and sequencing as described previously (44). The cDNA clones of dsRNAs 1-4 were initially obtained by random priming of methyl mercuric hydroxide-denatured dsRNA using the Froussard procedure (45) with further DNA manipulations being performed according to standard protocols (46).…”

“…The cDNA clones of dsRNAs 1-4 were initially obtained by random priming of methyl mercuric hydroxide-denatured dsRNA using the Froussard procedure (45) with further DNA manipulations being performed according to standard protocols (46). For the synthesis of additional cDNAs covering their complete sequence, purified dsRNAs 1-4 were denatured with methyl mercuric hydroxide and subjected to a single-primer, genome-walking RT-PCR protocol as described previously (44). An RLM-RACE PCR procedure was used to determine the 5′-and 3′-terminal sequences of the dsRNAs (47).…”