Chianina beef tenderness investigated through integrated Omics

D’Alessandro, Angelo; Marrocco, Claudio; Rinalducci, Sara; Mirasole, Cristiana; Failla, S.; Zolla, Lello

doi:10.1016/j.jprot.2012.03.052

Cited by 84 publications

(48 citation statements)

References 112 publications

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“…Furthermore, our results were also consistent with those obtained in a similar study, in which Podolian cattle had higher abundance of TnT and MYL1, tougher beef and lower MFI and IMF content than crossbreed Romagnola×Podolian and Friesian cattle [17]. Likewise, MYL1 was more abundant in Chianina cattle classified as tough beef [13]. …”

Section: Resultssupporting

confidence: 91%

“…Likewise to what was observed in our study, higher level of ACTA1 phosphorylation was found in tough beef [13]. It has been suggested that phosphorylation of ACTA1 could prevent the onset of apoptosis and would be positively correlated with the meat toughness [11].…”

Section: Resultssupporting

confidence: 90%

“…However, the relation between abundance of energy metabolism enzymes and meat tenderness has been a controversial topic [10, 15, 32]. This discrepancy could be related to differences in enzyme activity, that may be altered by post-translational modifications such as phosphorylation [7, 12, 13]. …”

Section: Resultsmentioning

confidence: 99%

“…Phosphorylation stands out among the main post-translational modifications, and phosphoproteomics is a useful technique to study phosphorylated proteins. In muscle tissue, phosphorylation could modulate the interaction among myofilaments and the activity of metabolic enzymes [12, 13, 14]. …”

Section: Introductionmentioning

confidence: 99%

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Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

et al. 2017

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Proteins are the major constituents of muscle and are key molecules regulating the metabolic changes during conversion of muscle to meat. Brazil is one of the largest exporters of beef and most Brazilian cattle are composed by zebu (Nellore) genotype. Bos indicus beef is generally leaner and tougher than Bos taurus such as Angus. The aim of this study was to compare the muscle proteomic and phosphoproteomic profile of Angus and Nellore. Seven animals of each breed previously subjected the same growth management were confined for 84 days. Proteins were extracted from Longissimus lumborum samples collected immediately after slaughter and separated by two-dimensional electrophoresis. Pro-Q Diamond stain was used in phosphoproteomics. Proteins identification was performed using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Tropomyosin alpha-1 chain, troponin-T, myosin light chain-1 fragment, cytoplasmic malate dehydrogenase, alpha-enolase and 78 kDa glucose-regulated protein were more abundant in Nellore, while myosin light chain 3, prohibitin, mitochondrial stress-70 protein and heat shock 70 kDa protein 6 were more abundant in Angus (P<0.05). Nellore had higher phosphorylation of myosin regulatory light chain-2, alpha actin-1, triosephosphate isomerase and 14-3-3 protein epsilon. However, Angus had greater phosphorylation of phosphoglucomutase-1 and troponin-T (P<0.05). Therefore, proteins involved in contraction and muscle organization, myofilaments expressed in fast or slow-twitch fibers and heat shock proteins localized in mitochondria or sarcoplasmic reticulum and involved in cell flux of calcium and apoptosis might be associated with differences in beef quality between Angus and Nellore. Furthermore, prohibitin appears to be a potential biomarker of intramuscular fat in cattle. Additionally, differences in phosphorylation of myofilaments and glycolytic enzymes could be involved with differences in muscle contraction force, susceptibility to calpain, apoptosis and postmortem glycolysis, which might also be related to differences in beef quality among Angus and Nellore.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

et al. 2017

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“…This has been demonstrated in two studies of beef from Maremmana (42) and Chianina cattle (43) , providing useful data on simultaneous changes in proteins and metabolites but also disparities in metabolism between the two breeds. It was suggested that the postmortem metabolic rates in Maremmana and Chianina cattle was breed-specific, which directly exerted impacts on the postmortem time that was accessible to tender meat and demonstrated a practical link between industrial needs and integrated omics tools.…”

Section: New Perspectives In the Search For Markersmentioning

confidence: 87%

Molecular Understanding of Meat Quality Through Application of Proteomics

Therkildsen

et al. 2014

Food Reviews International

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Proteomics, as a significant and potent post-genomic tool in the field of meat science, allows researchers to decipher underlying molecular mechanisms behind different meat quality traits. In recent years, growing attention has been paid to biomarkers accounting for meat quality attributes by means of potent proteomic technologies with the objective to gain deeper insight. This review will focus on the biomarkers of protein changes associated with the most important meat quality traits, including tenderness, color and water holding capacity. Molecular understanding of meat quality through application of proteomics will be conducive to generate a large amount of scientific knowledge that helps improve our understanding of meat quality, control and tailor better meat quality.

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Effect of inhibition of μ‐calpain on the myofibril structure and myofibrillar protein degradation in postmortem ovine muscle

Gao

et al. 2016

J Sci Food Agric

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Chianina beef tenderness investigated through integrated Omics

Cited by 84 publications

References 112 publications

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

Molecular Understanding of Meat Quality Through Application of Proteomics

Effect of inhibition of μ‐calpain on the myofibril structure and myofibrillar protein degradation in postmortem ovine muscle

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