Chiasmata and chromosome breakages are related to crossing over in Drosophila ananassae males

Goñi, Beatriz; Matsuda, Muneo; Tobari, Yoshiko N.

doi:10.1139/g06-106

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Meiotic chromosome configurations show two large similar metacentric pairs and a tangled multivalent association formed by the X, Y, and 4th chromosomes as previously reported (Hinton and Downs 1975;). The number of chiasmata and of isosite chromosome breakages recorded for the chromosomes 2 and 3 were combined as previously done by Goñi et al (2006). Table 2 shows the gene arrangements found in the isofemale lines of the populations studied.…”

Section: Resultsmentioning

confidence: 99%

Crossing over does occur in males ofDrosophila ananassaefrom natural populations

Goñi

Matsuda

Yamamoto

et al. 2012

Genome

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Spontaneous crossing over in males of Drosophila ananassae has been well demonstrated using F(1) individuals from crosses between marker stocks and wild type strains. However, the question of its occurrence in males from natural populations remained open. Here we present the cytological evidence that crossing over does occur in males of D. ananassae from two Brazilian populations, sampled nearly 21 years apart, and in two recently sampled populations, one from Indonesia and one from Okinawa, Japan. Cytological analysis of meiosis in males collected from nature and in sons of females from the same population inseminated in nature revealed the presence of chiasmata, inversion chiasmata, and isosite chromosome breakages in the diplotene cells in all sampled populations. These data demonstrate that reciprocal and nonreciprocal exchanges and chromosome breakages, previously reported as related events of male crossing over, do occur at variable frequencies among males from natural populations.

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Section: Resultsmentioning

confidence: 99%

Crossing over does occur in males ofDrosophila ananassaefrom natural populations

Goñi

Matsuda

Yamamoto

et al. 2012

Genome

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“…Possibly related to this NO localization is the fact that, in the primary spermatocyte, the X, Y, and fourth chromosomes form a tangled multivalent (Hinton and Downs 1975;Matsuda et al 1983;Goni et al 2006). Another unique character of the species is that spontaneous crossing over occurs in males (Kikkawa 1937;Moriwaki 1937), albeit at much lower frequencies than in females.…”

Section: Ananassae Mapsmentioning

confidence: 99%

Polytene Chromosomal Maps of 11 Drosophila Species: The Order of Genomic Scaffolds Inferred From Genetic and Physical Maps

et al. 2008

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The sequencing of the 12 genomes of members of the genus Drosophila was taken as an opportunity to reevaluate the genetic and physical maps for 11 of the species, in part to aid in the mapping of assembled scaffolds. Here, we present an overview of the importance of cytogenetic maps to Drosophila biology and to the concepts of chromosomal evolution. Physical and genetic markers were used to anchor the genome assembly scaffolds to the polytene chromosomal maps for each species. In addition, a computational approach was used to anchor smaller scaffolds on the basis of the analysis of syntenic blocks. We present the chromosomal map data from each of the 11 sequenced non-Drosophila melanogaster species as a series of sections. Each section reviews the history of the polytene chromosome maps for each species, presents the new polytene chromosome maps, and anchors the genomic scaffolds to the cytological maps using genetic and physical markers. The mapping data agree with Muller's idea that the majority of Drosophila genes are syntenic. Despite the conservation of genes within homologous chromosome arms across species, the karyotypes of these species have changed through the fusion of chromosomal arms followed by subsequent rearrangement events. O NE of the primary strengths of the genus Drosophila as a model system has been the relative ease of generating detailed cytogenetic maps. Indeed, the first definitive mapping of genes to chromosomes Genetics 179: 1601-1655 ( July 2008) was performed in Drosophila melanogaster (Bridges 1916). The subsequent discovery of polytene chromosomes in the salivary glands in this same species (Painter 1934) and their codification into fine-structure genetic/ cytogenetic maps represents perhaps one of the first forays into ''genomics.'' Polytene maps (Bridges 1935;Lefevre 1976) provided an important genetic tool for mapping genes, for detecting genetic diversity within populations, and for inferring phylogenies among related species (Dobzhansky and Sturtevant 1938;Judd et al. 1972;Ashburner and Lemeunier 1976;Lemeunier and Ashburner 1976). Sturtevant and Tan (1937) laid the groundwork for comparative genomics when they established that genes within the chromosomal arms are conserved or syntenic among species. In an insightful melding of the gene mapping and evolutionary studies, H. J. Muller (1940) proposed that the genomes of Drosophila species were subdivided into a set of homologous elements represented by chromosome arms. What Muller (1940) noted, which was subsequently elaborated on by Sturtevant and Novitski (1941), was that the presumed homologs of identified mutant alleles within a chromosome arm of D. melanogaster were also confined to a single arm in other species within the genus where mapping data were available. Using D. melanogaster as a reference, Muller proposed that each of the five major chromosome arms plus the dot chromosome be given a letter designation (A-F) and that this nomenclature be used to identify equivalent linkage groups within the genus.The an...

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Male recombination in Brazilian populations ofDrosophila ananassae

Goñi

Matsuda

Tobari

2016

Genome

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With few exceptions, spontaneous crossing over does not normally occur in male Drosophila. Drosophila ananassae males show considerable amounts of crossing over. In wild males of D. ananassae from Asian (2008) and Brazilian populations (1986 and 2007) variable frequencies of meiotic crossing over, estimated from chiasmata counts, suggested the existence of factors controlling male crossing over in these populations. To corroborate for such prediction, we present data on spontaneous recombination in F1 males of D. ananassae heterozygous for chromosomes of the same Brazilian populations (1986) and marker chromosomes using three testers stocks. Mean recombination value was low, although high variability existed between individual frequencies. Recombination frequencies between lines in each tester stock were not significantly different, excepting when the 3ple-px and 3ple-cy testers were compared (p < 0.05). These two testers differ in respect to the regional distribution of crossovers. The occurrence of recombination in chromosomes 2 and 3 in F1 males tested with e(65) se; bri ru was not related, suggesting they are under independent genetic control. Our data are consistent with proposed genetic factors controlling male crossing over in the tester stocks and to the presence of enhancers and suppressors of male crossing over segregating in the Brazilian populations (1986).

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Chiasmata and chromosome breakages are related to crossing over in Drosophila ananassae males

Cited by 3 publications

References 18 publications

Crossing over does occur in males ofDrosophila ananassaefrom natural populations

Crossing over does occur in males ofDrosophila ananassaefrom natural populations

Polytene Chromosomal Maps of 11 Drosophila Species: The Order of Genomic Scaffolds Inferred From Genetic and Physical Maps

Male recombination in Brazilian populations ofDrosophila ananassae

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