Chick bioassay of Lysine

Ho, Uwaegbute; Lewis, D.

doi:10.1080/00071666608415631

Cited by 12 publications

References 11 publications

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Chick bioassay of available methionine and methionine plus cystine. Development of assay procedure

1975

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A series of experiments has been conducted with young chicks to establish a valid assay procedure' for assessing available sulphur amino acids (SAA) in protein concentrates. A basal diet was established in which soya bean meal provided 20% crude protein. This diet was shown to be limiting in only SAA. When adequately supplemented with methionine or methionine plus cystine it promoted excellent chick growth. The adaptation of the diet for the assay purposes is described. The linear portion of the regression curve of response on a logarithmic dose scale for methionine alone was found to lie between 0.015 and 0.25 %; and 0.009 and 0.09% for methionine in the presence of 0.4% cystine. These regions constituted the potential ranges for the bioassay of methionine and SAA.

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Chick bioassay of available methionine and methionine plus cystine. Development of assay procedure

1975

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The availabilities of lysine, methionine and tryptophan in Pruteen by chick growth assay

1980

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The concentrai:ions of available lysine, methionine and tryptophan in the single-cell protein, Pruteena (ICI), have been determined by growth assay with young chicks. Symmetrical, six-point, parallel-line assays were used with weight gain and gain: food ratio as responses. All diets were balanced as far as possible, especially with respect to essential amino acid composition. Concurrently with the determination of each amino acid in P'ruteen, its contents in typical fish and soya bean meals were determined. The assays were acceptable by the statistical criteria used. They yielded estimates of potencies that were realistic in relation to the total contents of lysine, methionine and tryptophan in Pruteen, fish and soya bean meals. The variances of potency estimates were typical of such assays. The values obtained for Pruteen considering both weight gain and gain:food ratio as responses, were about 40 g lysine, 18 g methionine anti 8.7 g tryptophan k g l air-dry matter. For lysine and methionine these represent high availabilities of total amino acids. The availability of tryptophan is less clear because of uncertainty about the total content of tryptophan in Pruteen.

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Bioavailability of lysine in rapeseed and soyabean meals determined by digestibility trial in cockerels and chick growth assay

Larbier

Chagneau

Lessire

1991

Animal Feed Science and Technology

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Chick bioassay of Lysine

Cited by 12 publications

References 11 publications

Chick bioassay of available methionine and methionine plus cystine. Development of assay procedure

Chick bioassay of available methionine and methionine plus cystine. Development of assay procedure

The availabilities of lysine, methionine and tryptophan in Pruteen by chick growth assay

Bioavailability of lysine in rapeseed and soyabean meals determined by digestibility trial in cockerels and chick growth assay

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