Chicken anaemia virus antibody ELISA: Problems with non‐specific reactions

Michalski, Wojtek P.; O’Rourke, Denise; Bagust, T. J.

doi:10.1080/03079459608419139

Cited by 11 publications

(7 citation statements)

References 12 publications

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“…Results obtained from the experimental and control birds sacrificed on day 3 and day 5 were considered as non-specific false positives, based on our consistent negative findings on routine histopathologic and immunoperoxidase staining. On the basis of our own findings and some of the relevant literature [19,22], it has been suggested that in 1-day-old chicks inoculated with CIAV, seropositivity by ELISA might be obtained as early as day 7 or 8, while consistent positive results might be achieved starting at day 14.…”

Section: Discussionsupporting

confidence: 57%

Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease

Kuşçu

Gürel

2008

J Vet Sci

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One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow, were observed. Tissue sections of thymus and bone marrow were stained using the streptavidin-biotin peroxidase method and examined under light microscope for evaluation of antigenic intensities in tissues. Those findings were then compared with blood parameters and ELISA results obtained through collected sera during sacrifice procedures. We sought to determine: the localization of viral antigens in thymus and bone marrow tissues after inoculation, the correlation between antigen intensities and hematologic, serologic and histopathologic findings, definitive diagnostic criteria using histopathologic and immunoperoxidase methods, and the reliability of these methods in the diagnosis of CIAV infection. For this purpose, 83, one-day-old SPF chicks were used. The birds were divided into experimental (n = 52) and control (n = 26) groups. A virus dose of TCID50 of 100,000/ml was administered intramuscularly to every bird in the experimental group. Based on the results of this study, we have suggested that clinical examination, along with macroscopic and microscopic evaluation of the thymus and bone marrow, maybe undertaken starting from day 7 post-inoculation (PI). ELISA, might be of value, as it might give consistent results starting from day 14 PI. However, the most reliable results were obtained through examination of thymus and bone marrow sections from infected birds stained by immunoperoxidase technique, as early as day 4 PI.

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Section: Discussionsupporting

confidence: 57%

Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease

Kuşçu

Gürel

2008

J Vet Sci

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“…Using the polyclonal antibody amplifies the nonspecific reactions with the allantoid fluid proteins, results also found in other studies (6,7,14). In the present study, it was necessary to precipitate the chicken serum proteins, especially the IgM isotype, with 1% trichloroacetic acid to avoid nonspecific protein binding (15).…”

Section: Reproducibility Of Lpb-elisasupporting

confidence: 55%

A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus

Cardoso

Mouro-Sousa

Oliveira³

et al. 1999

Braz J Med Biol Res

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A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r 2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88%), specificity (100%) and agreement (95.31%).

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“…ELISA has a distinct advantage in this aspect (Lamichhane et al, 1992;Tannock et al, 2003;Todd et al, 1990Todd et al, , 1999; however, ELISA is also laborious and time-consuming both for setting-up and for completion. Commercial ELISA kits are available for the detection of antibodies to CAV, albeit not in Japan; however, instances of false-positive or false-negative results have been reported (Michalski et al, 1996;Tannock et al, 2003). All these tests require specialized equipment or facilities.…”

Section: Discussionmentioning

confidence: 98%

“…Moreover, the ELISA necessitates the purification or semi-purification of antigens from infected cells. Compared to the IFAT, higher rates of false-positive reactions were obtained with certain commercial ELISA kits (Michalski et al, 1996;Tannock et al, 2003). Therefore, the development of a reliable, simple, and rapid test for the detection of antibodies to CAV is of utmost importance.…”

Section: Introductionmentioning

confidence: 98%

Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus

Trinh

Ogawa

Bui

et al. 2015

Journal of Virological Methods

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Chicken anaemia virus antibody ELISA: Problems with non‐specific reactions

Cited by 11 publications

References 12 publications

Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease

Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease

A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus

Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus

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