Chicken anemia virus VP1 negatively regulates type I interferon via targeting interferon regulatory factor 7 of the DNA-sensing pathway

Molecular characterization of a novel mitochondrial NOD-like receptor X1 in chicken that negatively regulates IFN-β expression via STING

“…The antibodies used were mouse anti-Myc, mouse anti-GFP, mouse anti-β-actin, mouse anti-GAPDH, goat anti-mouse, and goat anti-rabbit HRP-IgG (Enogene Biotech, Nanjing, China). Rabbit anti-NLRX1, anti-STING, and anti-TBK1 polyclonal antibodies were prepared according to a methodology similar to that described in a previous study ( Liu et al, 2023 ). Approval for the animal studies was obtained from the animal ethics committee of Anhui Agricultural University (Approval No.…”

“…The method was performed according to previous studies ( Liu et al, 2023 ). Briefly, 5 × 10 4 chicken HD11 cells per well in a 24-well plate were transiently transfected with 100 ng firefly luciferase reporter plasmid IFN-β-luc, 50 ng TK-Renilla luciferase reporter as an internal control, with 500 ng indicated plasmids for chNLRX1 or control vector together with STING expression using lipofectamine transfection reagent (Hanbio Biotechnology, Shanghai, China).…”

IRF7: role and regulation in immunity and autoimmunity

“…In addition to NFATC3, PEDV membrane protein, ORF45, VP23 and LMP1 promote the dimerization and nuclear translocation of IRF7, and other viral proteins, such as Us3, nonstructural protein(NSP) 2 of H1N1 influenza A virus, avian reovirus sA protein and the structural protein VP1 of chicken anemia virus, decrease the dimerization and nuclear translocation of IRF7 (95)(96)(97).…”