Chicken feather hydrolysate as alternative peptone source for microbial cultivation

Akpor, Oghenerobor B.; Odesola, Damilola E.; Thomas, Remilekun E.; Oluba, Olarewaju M.

doi:10.12688/f1000research.17134.1

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…Feather biomass consists of 90% of protein [ 1 ], which is a valuable biological product, and feather hydrolysates can be a source of peptones [ 2 , 3 ]. The feather is rich in essential amino acids leucine, valine, arginine, isoleucine, phenylalanine, and threonine, with smaller proportions of lysine, methionine, histidine, and tryptophan [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Bacillus sp. A5.3 Strain with Keratinolytic Activity

Aktayeva

Baltin

Kiribayeva

et al. 2022

Biology

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Environmental safety and economic factors necessitate a search for new ways of processing poultry farm feathers, which are 90% β-keratin and can be used as a cheap source of amino acids and peptones. In this study, feather-decomposing bacteria were isolated from a site of accumulation of rotten feathers and identified as Bacillus. Among them, the Bacillus sp. A5.3 isolate showed the best keratinolytic properties. Scanning electron microscopy indicated that Bacillus sp. A5.3 cells closely adhere to the feather surface while degrading the feather. It was found that Bacillus sp. A5.3 secretes thermostable alkaline proteolytic and keratinolytic enzymes. Zymographic analysis of the enzymatic extract toward bovine serum albumin, casein, gelatin, and β-keratin revealed the presence of proteases and keratinases with molecular weights 20–250 kDa. The proteolytic and keratinolytic enzymes predominantly belong to the serine protease family. Proteome analysis of the secreted proteins by nano-HPLC coupled with Q-TOF mass spectrometry identified 154 proteins, 13 of which are proteases and peptidases. Thus, strain Bacillus sp. A5.3 holds great promise for use in feather-processing technologies and as a source of proteases and keratinases.

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Section: Introductionmentioning

confidence: 99%

Isolation of Bacillus sp. A5.3 Strain with Keratinolytic Activity

Aktayeva

Baltin

Kiribayeva

et al. 2022

Biology

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Using inexpensive substrate to achieve high-level lipase A secretion by Bacillus subtilis through signal peptide and promoter screening

Cha

et al. 2020

Process Biochemistry

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Fabrication and characterization of keratin starch biocomposite film from chicken feather waste and ginger starch

Oluba

Chibugo

Akpor

et al. 2021

Sci Rep

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The disposal of chicken feather through burning or burying is not environmentally compliant due to the accompanying release of greenhouse gas and underground water contamination. Thus, the transformation of this bio-waste into a bio-composite film is considered not only a sustainable strategy for disposal of this solid wastes but also an attractive alternative to developing an efficient nanostructured biomaterial from renewable bio resource. In the present study keratin extracted from chicken feather waste in combination with ginger starch were fabricated into a bio-composite film. The fabricated bio-composite films were characterized, using different analytical techniques. The physicochemical characteristics of ginger starch showed a moisture content of 33.8%, pH of 6.21, amylose and amylopectin contents of 39.1% and 60.9%, respectively. The hydration capacity of the starch was 132.2% while its gelatinization temperature was 65.7 °C. Physical attributes of the bio-composite film, such as surface smoothness and tensile strength increased significantly (p < 0.05) with increasing keratin content, while its transparency and solubility showed significant (p < 0.05) decrease with increasing keratin level. The various blends of the bio-composite films decayed by over 50% of the original mass after 12 days of complete burial in soil. Based on the results obtained in this study, the addition of keratin to starch bio-composite showed remarkable improvement in mechanical properties, such as tensile strength and surface smoothness. The bio-composite film exhibited appropriate stability in water, although future study should be carried out to evaluate its thermal stability. Nonetheless, the fabricated keratin-starch bio-composite showed desirable characteristics that could be optimized for industrial applications.

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Chicken feather hydrolysate as alternative peptone source for microbial cultivation

Cited by 4 publications

References 19 publications

Isolation of Bacillus sp. A5.3 Strain with Keratinolytic Activity

Isolation of Bacillus sp. A5.3 Strain with Keratinolytic Activity

Using inexpensive substrate to achieve high-level lipase A secretion by Bacillus subtilis through signal peptide and promoter screening

Fabrication and characterization of keratin starch biocomposite film from chicken feather waste and ginger starch

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