Chicken Infectious Anemia in Mexico: Virus Identification and Serology Survey

Ledesma, Nestor; At, Fehervari; Mt, Casaubon; Lúcio, Érica Chaves; Rátz, F.

doi:10.2307/1592858

Cited by 10 publications

(3 citation statements)

References 21 publications

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“…On the other hands, clinical signs of the disease, especially in chicks above 3 weeks were not seen, but lesions suggestive of CAV such as both lymphoid depletion and atrophy of thymus were recorded. These findings were compatible with that previously mentioned by Ledesma et al (2001) who noticed that the chicks infected with virulence strain or high doses of virus after the decay of maternal antibodies suffered from lymphoid lesions without anemia. Moreover, the previous studies noticed the absence of clinical signs after about 3 weeks of age and that the immunocompetent chickens are resistant to disease, but they can acquire asymptomatic infections (Owoade et al, 2004;Schat, 2003).…”

Section: Discussionsupporting

confidence: 93%

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Hussein¹,

Arafa²,

Anwar³

et al. 2016

Adv. Anim. Vet. Sci.

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Section: Discussionsupporting

confidence: 93%

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Hussein¹,

Arafa²,

Anwar³

et al. 2016

Adv. Anim. Vet. Sci.

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“…Chicken anaemia virus (CAV) is an economically important avian pathogen with a worldwide distribution (AboElkhair et al, 2014;De Herdt et al, 2001;Farkas et al, 1998, Ganar et al, 2017Ledesma et al, 2001;McNulty 1991;Olszewska-Tomczyk et al, 2016;Rehman et al, 2011;Stanislawek and Howell, 1994;Zhou et al, 1996). The virus causes aplastic anaemia and generalised lymphoid atrophy with a concomitant immune suppression characterised by secondary bacterial, viral infections or vaccination failures (Adair, 2000;Bülow et al, 1983;Engstrom and Luthman 1984;Rosenberger and Cloud 1989;Schat, 2003).…”

Section: Introductionmentioning

confidence: 99%

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

et al. 2018

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Four DNA fragments (fragments A, B, C and D) covering the whole genome of chicken anemia virus (CAV) were amplified enzymatically by polymerase chain reaction (PCR) using four pairs of oligonucleotide primers. The DNA fragments were amplified from each of nine CAV isolates including eight Malaysian isolates and one European isolate (Cux-1). For all nine CAV isolates, fragment A (1518 bp) was digested with one restriction enzyme, Eco130I (StyI); fragment B (926 bp) with three enzymes, Eco130I (StyI), HpaII and MboI separately; fragment C (675 bp) with also three enzymes, BsuRI (HaeIII), HinfI, and HpaII separately; and the fragment D (552 bp) with one enzyme, EcoRI. Enzyme digested products of different fragments were separated by agarose gel or polyacrylamide gel electrophoresis. Each of the eightenzymatic reactions differentiated at least two isolates except the HpaII digestion of fragment C where no isolate was distinguished. The overall restriction endonuclease (RE) analysis separated four isolates (BL-1, BL-2, BL-4 and BL-5) in one group and the rest five isolates (SMSC-1, SMSC-2, 3-1, BL-3 and Cux-1) were differentiated from each other and also from the group of four isolates, based on the number of restriction site differences and the fragments generated by different enzymatic digestions. The study revealed that RE analysis could be used to identify and differentiate CAV isolates based on the number of restriction site differences. The study showed that more isolates, even the isolates from the same poultry farm, could be differentiated with proper genomic diversity after RE analysis of more genome fragments compared to that of single genome fragment.

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“…CAV was recorded in different countries allover the world (England, Mexico, Belgium, Brazil, Taiwan, Argentina, China, Israel, Nigeria, Greece, Malaysia, New Zealand, Iran, Turkey andIndia (Pope, 1991, Yong et al, 1993;Buscaglia et al, 1994;Zhou et al, 1997;Ledesma et al, 2001;Davidson et al, 2004;Oluwayelu et al, 2005;Bougiouklis et al, 2007;Hailemariam et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity and immunosuppressive potential of an Egyptian field isolate (2015) of the chicken anemia virus (CAV) in chickens

Nassif

Latif

El-Sayed

et al. 2017

Journal of Veterinary Medical Research

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Chicken anemia virus (CAV) is the causative agent of chicken infectious anemia (CIA). Studying CAV isolates in Egypt and their genetic diversity and its potential role in vaccination failure recently noticed in broiler flocks, is lacking in Egypt. So, the present study aimed to characterize CAV isolate-collected from a commercial broiler flock suffered from severe anemia and high mortality based on sequence and phylogenetic analysis of partial VP1 gene as well as to study pathogenicity and immunosuppressive potential in one day-old SPF chicks. The CAV isolate proved to be positive by PCR. The PCR product was sequenced and submitted to the gene bank under the accession number KX171350 and the CAV strain was designated as CLEVB-Zag2. Phylogenetic analysis at the nucleotide and amino acids level classify CLEVB-Zag2 CAV under group III (genotype III) of CAVs. On the other hand, the CLEVB-Zag2 CAV was found to be highly pathogenic for the experimentally inoculated SPF-chicks showing depression, severe anemia in almost all chicks in two infected groups beginning at the 7th day post infection (PI) and reached the peak of severity at the 14th day (hematocrit value, hemoglobin conc. and RBCs counts) are significantly reduced in chicks of the infected group. Blue wings were observed in few chicks in each infected group at the 14th day PI. Macroscopic lesions consisting of subcutaneous and muscular hemorrhages are observed with pale bone marrow, significant atrophy of thymus, spleen and bursa, hepatomegally with mottled liver and paleness of the carcasses were detected 7 days PI Those findings were evident and increased in severity until the day 14 PI. Concerning the CLEVB-Zag2 CAV, it was found to be highly immunosuppressive in the infected SPFchicks vaccinated with a commercial potent inactivated H5N1 vaccine as manifested by a significant reduction (protection 50%). The variance in the titer of the shieded challenge H5N1 virus was 1.45 log10 and the mean HI titer at the 4th week post vaccination was 1.5 log2 compared with the non-infected vaccinated group where these values were 90%, 2.35 log10 and 5.3 log2; respectively. In conclusion, the present study revealed that the CLEVB-Zag2 CAV isolate is highly pathogenic and immunosuppressive.

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Chicken Infectious Anemia in Mexico: Virus Identification and Serology Survey

Cited by 10 publications

References 21 publications

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

Pathogenicity and immunosuppressive potential of an Egyptian field isolate (2015) of the chicken anemia virus (CAV) in chickens

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